Secondary structure vs. temperature.

<p>Average number of paired nucleotides predicted by RNAfold as a function of optimal growth temperature for tRNA (A), rRNA (B), and mRNA (C). Results from random sequences are shown in open symbols.</p

Direct comparison of ASA fluctuation for different types of RNA.

<p>The average of standard deviation of solvent accessible surface area (ASA) by RNAsnap as a function of optimal growth temperature for random sequences (A) and natural sequences (B).</p

Direct comparison of ASA for different types of RNA.

<p>The average solvent accessible surface area (ASA) by RNAsnap as a function of optimal growth temperature for random sequences (A) and natural sequences (B). The relation between ASA and temperature after removing the reference ASA from random sequences is shown in (C).</p

Primary structure vs. Temperature.

<p>Average GC content as a function of optimal growth temperature for tRNA (A), rRNA (B), and mRNA (C).</p

Predicted (Red) versus actual (Black) solvent accessible surface area (ASA) of an RNA along with its structure colored coded for accessibility.

<p>Results of a corresponding random sequence (randomly shuffled from the original sequence) were shown in Blue. (A) Lysine-tRNA from <i>E</i>. <i>coli</i> (PDB: 5IB8, chain: 1K), (B) 5S RNA from <i>Thermus thermophilus</i> (PDB: 5IB8, chain: 16) and (C) <i>E coli’s</i> mRNA in the same complex (PDB: 5IB8, chain: 4L) Experimental structures were color-coded according to the relative ASA: 5+ for Red, 4 for Orange, 3 for Yellow, 2 for Green and 1 for Blue.</p

ASA fluctuation vs. Temperature.

<p>The standard deviation of solvent accessible surface area (ASA) by RNAsnap as a function of optimal growth temperature for tRNA (A), rRNA (B), and mRNA (C). Results from random sequences are shown in open symbols.</p

Tertiary structure vs. temperature.

<p>The average solvent accessible surface area (ASA) by RNAsnap as a function of optimal growth temperature for tRNA (A), rRNA (B), and mRNA (C). Results from random sequences are shown in open symbols. Colored points are results directly calculated from known tRNA and rRNA structures as labeled.</p