Identification of CDK2 substrates in human cell lysates

Engineered kinases and thiophosphate enrichment were used to identify many candidate CDK2 substrates in human cell lysates

Under-Sodium Viewing: A Review of Ultrasonic Imaging Technology for Liquid Metal Fast Reactors

This current report is a summary of information obtained in the "Information Capture" task of the U.S. DOE-funded "Under Sodium Viewing (USV) Project." The goal of the multi-year USV project is to design, build, and demonstrate a state-of-the-art prototype ultrasonic viewing system tailored for periodic reactor core in-service monitoring and maintenance inspections. The study seeks to optimize system parameters, improve performance, and re-establish this key technology area which will be required to support any new U.S. liquid-metal cooled fast reactors

Glucosamine and Glucosamine-6-phosphate Derivatives: Catalytic Cofactor Analogues for the <i>glmS</i> Ribozyme

Two analogues of glucosamine-6-phosphate (GlcN6P, <b>1</b>) and five of glucosamine (GlcN, <b>2</b>) were prepared for evaluation as catalytic cofactors of the <i>glmS</i> ribozyme, a bacterial gene-regulatory RNA that controls cell wall biosynthesis. Glucosamine and allosamine with 3-azido substitutions were prepared by S_N2 reactions of the respective 1,2,4,6-protected sugars; final acidic hydrolysis afforded the fully deprotected compounds as their TFA salts. A 6-phospho-2-aminoglucolactam (<b>31</b>) was prepared from glucosamine in a 13-step synthesis, which included a late-stage POCl₃-phosphorylation. A simple and widely applicable 2-step procedure with the triethylsilyl (TES) protecting group was developed to selectively expose the 6-OH group in N-protected glucosamine analogues, which provided another route to chemical phosphorylation. Mitsunobu chemistry afforded 6-cyano (<b>35</b>) and 6-azido (<b>36</b>) analogues of GlcN-(Cbz), and the selectivity for the 6-position was confirmed by NMR (COSY, HMBC, HMQC) experiments. Compound <b>36</b> was converted to the fully deprotected 6-azido-GlcN (<b>37</b>) and 2,6-diaminoglucose (<b>38</b>) analogues. A 2-hydroxylamino glucose (<b>42</b>) analogue was prepared via an oxaziridine (<b>41</b>). Enzymatic phosphorylation of <b>42</b> and chemical phosphorylation of its 6-OH precursor (<b>43</b>) were possible, but <b>42</b> and the 6-phospho product (<b>44</b>) were unstable under neutral or basic conditions. Chemical phosphorylation of the previously described 2-guanidinyl-glucose (<b>46</b>) afforded its 6-phospho analogue (<b>49</b>) after final deprotection

Under-Sodium Viewing: A Review of Ultrasonic Imaging Technology for Liquid Metal Fast Reactors

This current report is a summary of information obtained in the "Information Capture" task of the U.S. DOE-funded "Under Sodium Viewing (USV) Project." The goal of the multi-year USV project is to design, build, and demonstrate a state-of-the-art prototype ultrasonic viewing system tailored for periodic reactor core in-service monitoring and maintenance inspections. The study seeks to optimize system parameters, improve performance, and re-establish this key technology area which will be required to support any new U.S. liquid-metal cooled fast reactors