384 Array Metabolic Pathways.

<p>384 Array Metabolic Pathways.</p

Number of genes and corresponding R² for various ranges of RNA concentrations.

<p>Number of genes and corresponding R² for various ranges of RNA concentrations.</p

Individuals sampled over time.

<p>Heat map for one individual (00) sampled 4 times over a total of 6 weeks. Numbers above the heat map are time points (0, 2, 4 & 6 weeks) and the “P” is for p-value (−log₁₀). P-values are from the ANOVA that tested for differences among separate blood isolations within an individual (4 isolations and 8 replicates per isolation). For gene expression, red is greater and green is lower expression than the mean expression for each gene. Table provides number of significant genes and percent (rounded up) out of the total of 304. Color bar gives fold difference for log₂ gene expression (e.g., 2 = 4×) and negative log₁₀ p-value (e.g., 2 = p-value of 0.01).</p

Gene expression for Single Blood isolate.

<p>Heat map for single blood isolate that was divided into four. RNA was purified, labeled and hybridized separately for each sample. Red is greater and green is less than the average gene specific fluorescence. First column (P) is the p-value from a one-way ANOVA. Only 6 genes (2.3%) out of 252 are significant at a critical p-value of 0.01. P-values (−log₁₀) shown in the heat map are from an ANOVA for significant differences among samples using the 8 replicates for each separate RNA isolation. Color bar gives fold difference for log₂ gene expression (e.g., 2 = 4×) and negative log₁₀ p-value (e.g., 2 = p-value of 0.01).</p

Concentrations of Cy3 and Cy5 dye labeled RNA used for hybridization.

<p>Concentrations of Cy3 and Cy5 dye labeled RNA used for hybridization.</p