Body weight and glucose challenge.

<p>(A) Average weekly body weight across 15 weeks of experiment. (B) Glucose challenge (1 g/kg dextrose) at week 15. (C) Area under the curve for the glucose challenge. N = 20 (chow), 25 (HFCS-chow); ANOVA (panels A/B, repeated measures: group * sex * measurement timepoint; panel C: group * sex), * < .05, ** < .01, *** < .001, error bars S.E.M.</p

Recording sites for fast scan cyclic voltammetry recording.

<p>Blue circles are recording sites for Chow and red circles are recording sites of HFCS+Chow. Chow, n = 11; HFCS-Chow = 8.</p

Glucose challenge.

<p>(a) Weights after a 21 hour fast and prior to glucose challenge. (b) blood glucose levels at fasting baseline (timepoint 0) and following i.p. injection of dextrose (2 g/kg). N = 6 (no sucrose) and 8 (unlimited).</p

Concurrent choice.

<p>Average lever presses during (a) choice and (b) no choice conditions (n = 8). (c) Total food consumed (earned sucrose+freely available chow) during choice sessions (n = 8). (d) Percentage of total intake comprised of earned sucrose pellets during choice sessions (n = 8). Box plots: as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003221#pone-0003221-g001" target="_blank">figure 1</a>; * p<.05; **p<.005; *** p<.0001.</p

Experimental timeline, progressive ratio and activity.

<p>(a) Timeline indicating the order of experimental manipulations and tests. Two cohorts of mice were used and administered the experimental manipulation and testing procedures as indicated above and below the timeline. Breakpoint in progressive ratio (PR7) for the sucrose exposed and non-sucrose exposed mice tested under (b) food restriction and (c) sated conditions (n = 8). (d) Total distance traveled in the open field (1 hr) (n = 7). (e) Total number of wheel turns in 1 week (n = 7, 8). Box plots: Middle lines represent the median values, the top and bottom of the boxes represent the 25th and 75th percentiles, the whiskers represent the 10th and 90th percentiles and the dots represent mean values ; * p = .05.</p

High sugar/high fat dietary challenge.

<p>(a) Percent weight gain 3-weeks prior to (left) and during (right) the 3-week HS/HF exposure period in adulthood. (b) Body weight at the beginning and subsequent three weeks of HS/HF dietary options. (c) Metabolic efficiency as gram body weight increase per kcal consumed across the HS/HF dietary challenge. (d) Weekly consumption of standard chow (solid lines) and HS/HF options (dashed lines). N = 5–7; ±SEM, * p<.05.</p

Sucrose preference.

<p>(a) Average preference expressed as sucrose consumed (g) divided by total sucrose and water consumption (g) (n = 7). Dashed line indicates no preference. (b) Average preference for the bottle previously paired with sucrose during extinction (n = 7). (c) Average sucrose and (d) water consumption (n = 7) ±SEM; * p<.05, ** p = .005.</p