1 research outputs found
Assessment of Peeling of Astragalus Roots Using <sup>1</sup>H NMR- and UPLC-MS-Based Metabolite Profiling
A metabolomic
analysis was performed to examine the postharvest
processing of Astragalus membranaceus roots with a focus on the peeling procedure using <sup>1</sup>H
NMR and UPLC-MS analyses. Principal component analysis (PCA) score
plots from the <sup>1</sup>H NMR and UPLC-MS data showed clear separation
between peeled and unpeeled <i>Astragalus</i> roots. Peeled
roots exhibited significant losses of several primary metabolites,
including acetate, alanine, arginine, caprate, fumarate, glutamate,
histidine, <i>N</i>-acetylaspartate, malate, proline, sucrose,
trigonelline, and valine. In contrast, the peeled roots contained
higher levels of asparagine, aspartate, and xylose, which are xylem-related
compounds, and formate, which is produced in response to wound stress
incurred during postharvest processing. In addition, the levels of
isoflavonoids and astragalosides were significantly reduced in peeled <i>Astragalus</i> root. These results demonstrate that metabolite
profiling based on a combination of <sup>1</sup>H NMR and UPLC-MS
analyses can be used to evaluate peeling procedures used in the postharvest
processing of herbal medicines