3 research outputs found
Bioactive properties and phytochemical assessment of Bacupari-anão (Garcinia brasiliensis Mart.) leaves native to Rondônia, Brazil
Leaf fractions of Garcinia brasiliensis were evaluated concerning their antioxidant, antimicrobial, anti inflammatory and cytotoxic properties, and the most active fraction was then fully characterized regarding its phenolic composition using HPLC-DAD-ESI/MSn. The ethyl acetate fraction from partitioning of the methanolic leaf extract revealed a strong antioxidant activity that was comparable to Trolox, the positive control. This fraction was also able to show a significant antimicrobial activity against Gram-positive and Gram-negative bacteria and the fungus Candida albicans. However, the dichloromethane fraction was found to present the highest anti-inflammatory (83 ± 9 µg mL−1) and cytotoxic activities, thus presenting slight toxicity using a non-tumor cell line. Regarding the phenolic profile, the ethyl acetate fraction presented twelve flavonoids, with morelloflavone-7’’-O-glucoside (52.1 ± 0.4 mg g−1) and gardinia biflavonoid 2a glucoside (27.5 ± 0.2 mg g−1) being the major compounds identified. These results indicate that leaves of G. brasiliensis might be a potential source of natural biomolecules for pharmaceutical and medicinal applications.The authors are grateful to the Foundation for Science and
Technology (FCT, Portugal) and FEDER under Programme
PT2020 for financial support to CIMO (UID/AGR/00690/2013)
and L. Barros’s contract; to the FEDER-Interreg España-
Portugal programme for financial support through the project
0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
Valorising leaves of Garciniabrasiliensi Mart as sources of bioactive compounds
Discovery of new drugs from natural products is becoming widespread and an example of a promising
plant is the bacupari-anão or bacupari mirim that belongs to the species: Garcinia brasiliensi Mart, family
Myrtaceae. This tree is native to the forests of the Amazonian and also to the Atlantic Forest. Since
ancient time, the leaves of this tree have been reported to have numerous functionalities, correlated with
the presence of bioactive compounds [1]. Thus, this study aimed to evaluate the bioactive properties of
G. brasiliensi leaves regarding to its possible antioxidant activity and cytotoxic properties in human tumor
cells. Three different extracts of the leaves were tested: hexane, dichloromethane and ethyl acetate.
Furthermore, the most active extract was characterized in terms of its phenolic compounds
content.Chemical profile of the extracts was obtained using an HPLC system coupled to a diode array
detector (DAD) and mass spectrometry (MS) with an electrospray ionization interface (ESI). The
antioxidant activity was evaluated by four in vitro assays:DPPH (2,2-diphenyl-1-picrylhydrazyl) radical
scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation
by the thiobarbituric acid reactive substances (TBARS) assay.The cytotoxicity was tested in MCF-7
(breast adenocarcinoma), NCI-H460 (non-small cell lung carcinoma), HeLa (cervical carcinoma) and
HepG2 (hepatocellular carcinoma) cell lines and in a non-tumor primary culture (porcine liver primary
culture PLP2). Among all the tested samples, ethyl acetate extract presented the highest DPPH
scavenging activity (EC50 value = 31,2 ± 0,2μg/mL), reducing power (68,8± 0,2 μg/mL), β-carotene
bleaching inhibition capacity (15,9 ± 0,3μg/mL) and TBARS (4,6 ± 0,2 μg/mL). It was also able to inhibit
all the tested human tumor cells and none of the samples revealed toxicity for the non-tumor cell line
PLP2 (GI50<400 μg/mL). The ethyl acetate extract was the most active extract and analysis of HPLC-DADMS
data revealed a total of twelve phenolic compounds, comprising five bioflavonoids, four flavones, two
flavonols and a flavan-3-ol. The most abundant phenolic compound was the bioflavonoid
morelloflavone-7-O-glucoside. This study highlights the importance of the recovery and valorisation of G.
brasiliensi leaves, in order to obtain valuable products, which can be explored in the development of
functional foods.The authors are grateful to FCT, Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), A. Fernandes grant (SFRH/BPD/114753/2016), and L. Barros and R.Calhelha contracts; to Interreg España-Portugal for financial support through the project 0377_Iberphenol_6_E; and to the Federal Institute of Education, Science and Technology of Rondônia and the– CAPES.info:eu-repo/semantics/publishedVersio
Garcínía brasiliensis leaves: Bioactive properties and phytochemical assessment
The Amazon rainforest is the home to several medicinal plant species popularly used
for the treatment of a wide range of diseases. Despite the rich biodiversity of this
region, there is still a lack of comprehensive studies regarding the uses, bioactivities,
chemical composition and pharmacological properties of many of these medicinal
plants [l]. In the present work, Garcinia brasiliensis Mart., a green plant native to
the Amazon region, cultivated for its edible fruit, popularly referred to as "bacuparianão"
or "guapomo" was studied. Some of its well reported bioactive properties
include antioxidant, antidiabetic, anti-inflammatory, antispasmodic and antiprotozoal
activities but very little studies have been conducted describing the phytochemical
profile and establishing compounds-bioactivity relationships of the extracts obtained
from its vegetative organs [2]. In view of these pharmaceutical potentials, leaves of
G. brasilienses were collected at São Cristóvão site, Paraná, Brazil. They were then
submitted to a cold methanol extraction, and further liquid-liquid partitioned to
obtain hexane, dichloromethane and ethyl acetate fractions. The antioxidant, antiinflammatory, antimicrobial and cytotoxic activities of each fraction were tested, and
the most bioactive fraction was submitted to phenolic characterization using HPLCDAD-
ESI/MSn. The results showed that the ethyl acetate fraction showed the highest
antioxidant activity which was comparable to Trolox, the positive control. This fraction
also showed a broad spectrum antimicrobial activity against Gram-positive and Gramnegative
bactéria, and Cândida albicans. The phenolic profile of the ethyl acetate
fraction presented twelve flavonoids, being morelloflavone-7"-0-glucoside (52. 1±0.4
mg/g) and gardinia biflavonoid 2a glucoside (27. 5±0.2mg/g) the major compounds
identified. These results indicate that leaves of G. brasiliensis might be a potential
source of natural biomolecules for pharmaceutical and medicinal applications.The authors are grateful to the Foundation for Science and
Technology (FCT, Portugal) and FEDER under Programme PT2020 for financiai support to
CIMO (UID/AGR/00690/2013) and L. Barros contract; to FEDER-Interreg Espana-Portugal
programme for financiai support through the project 0377_Iberphenol_6_E.]info:eu-repo/semantics/publishedVersio