16 research outputs found
Maternal and Cord Blood LC-HRMS Metabolomics Reveal Alterations in Energy and Polyamine Metabolism, and Oxidative Stress in Very-low Birth Weight Infants
To
assess the global effect of preterm birth on fetal metabolism
and maternal–fetal nutrient transfer, we used a mass spectrometric-based
chemical phenotyping approach on cord blood obtained at the time of
birth. We sampled umbilical venous, umbilical arterial, and maternal
blood from mothers delivering very-low birth weight (VLBW, with a
median gestational age and weight of 29 weeks, and 1210 g, respectively)
premature or full-term (FT) neonates. In VLBW group, we observed a
significant elevation in the levels and maternal-fetal gradients of
butyryl-, isovaleryl-, hexanoyl- and octanoyl-carnitines, suggesting
enhanced short- and medium chain fatty acid β-oxidation in human
preterm feto-placental unit. The significant decrease in glutamine-glutamate
in preterm arterial cord blood beside lower levels of amino acid precursors
of Krebs cycle suggest increased glutamine utilization in the fast
growing tissues of preterm fetus with a deregulation in placental
glutamate-glutamine shuttling. Enhanced glutathione utilization is
likely to account for the decrease in precursor amino acids (serine,
betaine, glutamate and methionine) in arterial cord blood. An increase
in both the circulating levels and maternal–fetal gradients
of several polyamines in their acetylated form (diacetylspermine and
acetylputrescine) suggests an enhanced polyamine metabolic cycling
in extreme prematurity. Our metabolomics study allowed the identification
of alterations in fetal energy, antioxidant defense, and polyamines
and purines flux as a signature of premature birth
Maternal and Cord Blood LC-HRMS Metabolomics Reveal Alterations in Energy and Polyamine Metabolism, and Oxidative Stress in Very-low Birth Weight Infants
To
assess the global effect of preterm birth on fetal metabolism
and maternal–fetal nutrient transfer, we used a mass spectrometric-based
chemical phenotyping approach on cord blood obtained at the time of
birth. We sampled umbilical venous, umbilical arterial, and maternal
blood from mothers delivering very-low birth weight (VLBW, with a
median gestational age and weight of 29 weeks, and 1210 g, respectively)
premature or full-term (FT) neonates. In VLBW group, we observed a
significant elevation in the levels and maternal-fetal gradients of
butyryl-, isovaleryl-, hexanoyl- and octanoyl-carnitines, suggesting
enhanced short- and medium chain fatty acid β-oxidation in human
preterm feto-placental unit. The significant decrease in glutamine-glutamate
in preterm arterial cord blood beside lower levels of amino acid precursors
of Krebs cycle suggest increased glutamine utilization in the fast
growing tissues of preterm fetus with a deregulation in placental
glutamate-glutamine shuttling. Enhanced glutathione utilization is
likely to account for the decrease in precursor amino acids (serine,
betaine, glutamate and methionine) in arterial cord blood. An increase
in both the circulating levels and maternal–fetal gradients
of several polyamines in their acetylated form (diacetylspermine and
acetylputrescine) suggests an enhanced polyamine metabolic cycling
in extreme prematurity. Our metabolomics study allowed the identification
of alterations in fetal energy, antioxidant defense, and polyamines
and purines flux as a signature of premature birth
Maternal and Cord Blood LC-HRMS Metabolomics Reveal Alterations in Energy and Polyamine Metabolism, and Oxidative Stress in Very-low Birth Weight Infants
To
assess the global effect of preterm birth on fetal metabolism
and maternal–fetal nutrient transfer, we used a mass spectrometric-based
chemical phenotyping approach on cord blood obtained at the time of
birth. We sampled umbilical venous, umbilical arterial, and maternal
blood from mothers delivering very-low birth weight (VLBW, with a
median gestational age and weight of 29 weeks, and 1210 g, respectively)
premature or full-term (FT) neonates. In VLBW group, we observed a
significant elevation in the levels and maternal-fetal gradients of
butyryl-, isovaleryl-, hexanoyl- and octanoyl-carnitines, suggesting
enhanced short- and medium chain fatty acid β-oxidation in human
preterm feto-placental unit. The significant decrease in glutamine-glutamate
in preterm arterial cord blood beside lower levels of amino acid precursors
of Krebs cycle suggest increased glutamine utilization in the fast
growing tissues of preterm fetus with a deregulation in placental
glutamate-glutamine shuttling. Enhanced glutathione utilization is
likely to account for the decrease in precursor amino acids (serine,
betaine, glutamate and methionine) in arterial cord blood. An increase
in both the circulating levels and maternal–fetal gradients
of several polyamines in their acetylated form (diacetylspermine and
acetylputrescine) suggests an enhanced polyamine metabolic cycling
in extreme prematurity. Our metabolomics study allowed the identification
of alterations in fetal energy, antioxidant defense, and polyamines
and purines flux as a signature of premature birth
Maternal and Cord Blood LC-HRMS Metabolomics Reveal Alterations in Energy and Polyamine Metabolism, and Oxidative Stress in Very-low Birth Weight Infants
To
assess the global effect of preterm birth on fetal metabolism
and maternal–fetal nutrient transfer, we used a mass spectrometric-based
chemical phenotyping approach on cord blood obtained at the time of
birth. We sampled umbilical venous, umbilical arterial, and maternal
blood from mothers delivering very-low birth weight (VLBW, with a
median gestational age and weight of 29 weeks, and 1210 g, respectively)
premature or full-term (FT) neonates. In VLBW group, we observed a
significant elevation in the levels and maternal-fetal gradients of
butyryl-, isovaleryl-, hexanoyl- and octanoyl-carnitines, suggesting
enhanced short- and medium chain fatty acid β-oxidation in human
preterm feto-placental unit. The significant decrease in glutamine-glutamate
in preterm arterial cord blood beside lower levels of amino acid precursors
of Krebs cycle suggest increased glutamine utilization in the fast
growing tissues of preterm fetus with a deregulation in placental
glutamate-glutamine shuttling. Enhanced glutathione utilization is
likely to account for the decrease in precursor amino acids (serine,
betaine, glutamate and methionine) in arterial cord blood. An increase
in both the circulating levels and maternal–fetal gradients
of several polyamines in their acetylated form (diacetylspermine and
acetylputrescine) suggests an enhanced polyamine metabolic cycling
in extreme prematurity. Our metabolomics study allowed the identification
of alterations in fetal energy, antioxidant defense, and polyamines
and purines flux as a signature of premature birth
Maternal and Cord Blood LC-HRMS Metabolomics Reveal Alterations in Energy and Polyamine Metabolism, and Oxidative Stress in Very-low Birth Weight Infants
To
assess the global effect of preterm birth on fetal metabolism
and maternal–fetal nutrient transfer, we used a mass spectrometric-based
chemical phenotyping approach on cord blood obtained at the time of
birth. We sampled umbilical venous, umbilical arterial, and maternal
blood from mothers delivering very-low birth weight (VLBW, with a
median gestational age and weight of 29 weeks, and 1210 g, respectively)
premature or full-term (FT) neonates. In VLBW group, we observed a
significant elevation in the levels and maternal-fetal gradients of
butyryl-, isovaleryl-, hexanoyl- and octanoyl-carnitines, suggesting
enhanced short- and medium chain fatty acid β-oxidation in human
preterm feto-placental unit. The significant decrease in glutamine-glutamate
in preterm arterial cord blood beside lower levels of amino acid precursors
of Krebs cycle suggest increased glutamine utilization in the fast
growing tissues of preterm fetus with a deregulation in placental
glutamate-glutamine shuttling. Enhanced glutathione utilization is
likely to account for the decrease in precursor amino acids (serine,
betaine, glutamate and methionine) in arterial cord blood. An increase
in both the circulating levels and maternal–fetal gradients
of several polyamines in their acetylated form (diacetylspermine and
acetylputrescine) suggests an enhanced polyamine metabolic cycling
in extreme prematurity. Our metabolomics study allowed the identification
of alterations in fetal energy, antioxidant defense, and polyamines
and purines flux as a signature of premature birth
Maternal and Cord Blood LC-HRMS Metabolomics Reveal Alterations in Energy and Polyamine Metabolism, and Oxidative Stress in Very-low Birth Weight Infants
To
assess the global effect of preterm birth on fetal metabolism
and maternal–fetal nutrient transfer, we used a mass spectrometric-based
chemical phenotyping approach on cord blood obtained at the time of
birth. We sampled umbilical venous, umbilical arterial, and maternal
blood from mothers delivering very-low birth weight (VLBW, with a
median gestational age and weight of 29 weeks, and 1210 g, respectively)
premature or full-term (FT) neonates. In VLBW group, we observed a
significant elevation in the levels and maternal-fetal gradients of
butyryl-, isovaleryl-, hexanoyl- and octanoyl-carnitines, suggesting
enhanced short- and medium chain fatty acid β-oxidation in human
preterm feto-placental unit. The significant decrease in glutamine-glutamate
in preterm arterial cord blood beside lower levels of amino acid precursors
of Krebs cycle suggest increased glutamine utilization in the fast
growing tissues of preterm fetus with a deregulation in placental
glutamate-glutamine shuttling. Enhanced glutathione utilization is
likely to account for the decrease in precursor amino acids (serine,
betaine, glutamate and methionine) in arterial cord blood. An increase
in both the circulating levels and maternal–fetal gradients
of several polyamines in their acetylated form (diacetylspermine and
acetylputrescine) suggests an enhanced polyamine metabolic cycling
in extreme prematurity. Our metabolomics study allowed the identification
of alterations in fetal energy, antioxidant defense, and polyamines
and purines flux as a signature of premature birth
Effect of BPA exposure on germ cell differentiation in first trimester human fetal testis xenografts.
<p>Human fetal testes (9.1–11.3 GW) were xenografted into castrate Nude (host) mice. Host mice received vehicle (Control) or 10μM BPA in the drinking water for five weeks. (A) Histological sections of testes after immunostaining for AP-2γ (gonocytes). Positive (red arrows) and negative (black arrows) germ cells can be identified. Scale bar: 60 μm. (B) Quantification of AP-2γ-positive cells displayed as mean ± SEM (n = 9) on the left panel and as individual values with a line drawn between the control and the corresponding BPA-treated testis from the same fetus on the right panel. (C) Histological sections of testes after immunostaining for MAGE-A4 (prespermatogonia). Positive (red arrows) and negative (black arrows) germ cells can be identified. Scale bar: 60 μm. (D) Quantification of MAGE-A4-positive cells displayed as mean ± SEM (n = 8) on the left part and as individual values with a line drawn between the control and the corresponding BPA-treated testis from the same fetus on the right part. Data analyzed using the Wilcoxon paired-test. *p<0.05, **p<0.01.</p
Effect of BPA exposure on germ cell apoptosis and proliferation in first trimester human fetal testes cultured using the FeTA system.
<p>Human fetal testes (6–12 GW, mean 8.7 ± 0.6 GW) were cultured using the ex vivo <u>h</u>uman <u>Fe</u>tal <u>T</u>estis <u>A</u>ssay system (hFeTA). After 24 hours in control medium, explants were cultured with 100 ng/mL of LH for the 3 subsequent days in the presence of ethanol vehicle (control explants) or BPA at concentrations ranging from 0.01 to 10 μM. Control and BPA-treated explants were paired samples from the same testis. (A) Histological sections after labeling with anti-cleaved caspase-3 antibody (brown) and anti-AMH antibody (green). Positive (red arrows) and negative (black arrows) germ cells can be identified. Scale bar: 10 μm. (B) Quantification of cleaved caspase-3 positive cells (mean ± SEM; n = 4–8). (C) Histological sections after labeling with anti-Ki-67 antibody (brown) and anti-AMH antibody (green). Positive (red arrows) and negative (black arrows) germ cells can be identified. Scale bar: 50 μm. (D) Quantification of Ki67 positive gonocytes (mean ± SEM; n = 4–8).</p
Effect of BPA exposure on plasma BPA concentration in xenografted mice.
<p>Plasma levels of BPA were quantified by gas chromatography coupled to tandem mass spectrometry (GC-MS/MS) from castrated Nude male mice xenografted with first trimester human fetal testis (9.1–11.3 GW, mean 10.2 ± 0.2 GW; n = 6–7) and exposed to vehicle (Control) or BPA (10 μM in the drinking water) for five weeks. For each fetus, all the pieces from one testis were grafted in a control mouse and all the pieces from the contralateral testis were grafted in a BPA-treated mouse. Statistical analysis was performed using the Mann-Whitney test. *p<0.05, **p<0.01.</p
Effect of BPA exposure on germ cell density in first trimester human fetal testis xenografts.
<p>Human fetal testes (9.1–11.3 GW) were xenografted into castrate Nude (host) mice. Host mice received vehicle (Control) or 10μM BPA in the drinking water for five weeks. (A) Histological sections after haematoxylin-eosin-saffron staining. Germ cells (black arrow) can be easily identified. Scale bar: 20 μm. (B) Quantification of germ cell density displayed as mean ± SEM (n = 9) on the left panel and as individual values with a line drawn between the control and the corresponding BPA-treated testis from the same fetus on the right panel. Data analyzed using the Wilcoxon paired-test. *p<0.05 compared with control condition.</p