1 research outputs found
Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-galactose Glycan Biomarkers
The disaccharide motif fucose-α(1-2)-galactose
(Fucα(1-2)Gal)
is involved in many important physiological processes, such as learning
and memory, inflammation, asthma, and tumorigenesis. However, the
size and structural complexity of Fucα(1-2)Gal-containing glycans
have posed a significant challenge to their detection. We report a
new chemoenzymatic strategy for the rapid, sensitive detection of
Fucα(1-2)Gal glycans. We demonstrate that the approach is highly
selective for the Fucα(1-2)Gal motif, detects a variety of complex
glycans and glycoproteins, and can be used to profile the relative
abundance of the motif on live cells, discriminating malignant from
normal cells. This approach represents a new potential strategy for
biomarker detection and expands the technologies available for understanding
the roles of this important class of carbohydrates in physiology and
disease