The weight of phonetic substance in the structure of sound inventories

In the research field initiated by Lindblom & Liljencrants in 1972, we illustrate the possibility of giving substance to phonology, predicting the structure of phonological systems with nonphonological principles, be they listener-oriented (perceptual contrast and stability) or speaker-oriented (articulatory contrast and economy). We proposed for vowel systems the Dispersion-Focalisation Theory (Schwartz et al., 1997b). With the DFT, we can predict vowel systems using two competing perceptual constraints weighted with two parameters, respectively λ and α. The first one aims at increasing auditory distances between vowel spectra (dispersion), the second one aims at increasing the perceptual salience of each spectrum through formant proximities (focalisation). We also introduced new variants based on research in physics - namely, phase space (λ,α) and polymorphism of a given phase, or superstructures in phonological organisations (Vallée et al., 1999) which allow us to generate 85.6% of 342 UPSID systems from 3- to 7-vowel qualities. No similar theory for consonants seems to exist yet. Therefore we present in detail a typology of consonants, and then suggest ways to explain plosive vs. fricative and voiceless vs. voiced consonants predominances by i) comparing them with language acquisition data at the babbling stage and looking at the capacity to acquire relatively different linguistic systems in relation with the main degrees of freedom of the articulators; ii) showing that the places “preferred” for each manner are at least partly conditioned by the morphological constraints that facilitate or complicate, make possible or impossible the needed articulatory gestures, e.g. the complexity of the articulatory control for voicing and the aerodynamics of fricatives. A rather strict coordination between the glottis and the oral constriction is needed to produce acceptable voiced fricatives (Mawass et al., 2000). We determine that the region where the combinations of Ag (glottal area) and Ac (constriction area) values results in a balance between the voice and noise components is indeed very narrow. We thus demonstrate that some of the main tendencies in the phonological vowel and consonant structures of the world’s languages can be explained partly by sensorimotor constraints, and argue that actually phonology can take part in a theory of Perception-for-Action-Control

EFFECT OF A STRETCHING PROGRAM ON OVERHEAD ATHLETES WITH A STIFF AND PAINFUL SHOULDER

Overhead athletes are more at risk of suffering shoulder injuries or/and developing shoulder pain. Stretching programs have been shown to successfully reduce pain for this population. However no study has provided a global overview of the effects of stretching. The present study evaluated the evolution of the pain, the strength of the shoulder muscles, potential instabilities or lesion of the shoulder as well as the 3D scapular kinematics of 10 overhead athletes having a painful and stiff dominant shoulder. Our results show a significant decrease of the pain and of the positive impingement syndrome tests. The kinematics of the scapula reveals a more upward rotation of the scapula after the stretching, which may reduce the risk of sub-acromial conflicts. The strength of the internal and external rotators of the shoulder is not modified by the program

Kinetics of pore formation by the Bacillus thuringiensis toxin Cry1Ac

AbstractAfter binding to specific receptors, Cry toxins form pores in the midgut apical membrane of susceptible insects. The receptors could form part of the pore structure or simply catalyze pore formation and consequently be recycled. To discriminate between these possibilities, the kinetics of pore formation in brush border membrane vesicles isolated from Manduca sexta was studied with an osmotic swelling assay. Pore formation, as deduced from changes in membrane permeability induced by Cry1Ac during a 60-min incubation period, was strongly dose-dependent, but rapidly reached a maximum as toxin concentration was increased. Following exposure of the vesicles to the toxin, the osmotic swelling rate reached a maximum shortly after a delay period. Under these conditions, at relatively high toxin concentrations, the maximal osmotic swelling rate increased linearly with toxin concentration. When vesicles were incubated for a short time with the toxin and then rapidly cooled to prevent the formation of new pores before and during the osmotic swelling experiment, a plateau in the rate of pore formation was observed as toxin concentration was increased. Taken together, these results suggest that the receptors do not act as simple catalysts of pore formation, but remain associated with the pores once they are formed

The role of membrane-bound metal ions in toxicity of a human cancer cell-active pore-forming toxin Cry41Aa from Bacillus thuringiensis

Bacillus thuringiensis crystal (Cry) proteins, used for decades as insecticidal toxins, are well known to be toxic to certain insects, but not to mammals. A novel group of Cry toxins called parasporins possess a strong cytocidal activity against some human cancer cells. Cry41Aa, or parasporin3, closely resembles commercially used insecticidal toxins and yet is toxic to the human hepatic cancer cell line HepG2, disrupting membranes of susceptible cells, similar to its insecticidal counterparts. In this study, we explore the protective effect that the common divalent metal chelator EGTA exerts on Cry41Aa's activity on HepG2 cells. Our results indicate that rather than interfering with a signalling pathway as a result of chelating cations in the medium, the chelator prevented the toxin's interaction with the membrane, and thus the subsequent steps of membrane damage and p38 phosphorylation, by removing cations bound to plasma membrane components. BAPTA and DTPA also inhibited Cry41Aa toxicity but at higher concentrations. We also show for the first time that Cry41Aa induces pore formation in planar lipid bilayers. This activity is not altered by EGTA, consistent with a biological context of chelation. Salt supplementation assays identified Ca2+, Mn2+ and Zn2+ as being able to reinstate Cry41Aa activity. Our data suggest the existence of one or more metal cation-dependent receptors in the Cry41Aa mechanism of action

Protein-tyrosine phosphatase SHP2 is positively linked to proteinase-activated receptor 2-mediated mitogenic pathway.

Proteinase-activated receptor-2 (PAR2), a new member of family of the G protein-coupled receptors, is activated by proteolytic cleavage of its extracellular amino terminus, a mechanism similar to that used by the thrombin receptor. It has been suggested that PAR2 has a potential role in the late phases of the acute inflammatory response and in tissue repair and/or skin-related disorders. Here we demonstrate that the agonist peptide (SLIGRL) stimulated c-fos-mediated mitogenic activation and tyrosine phosphorylation of cellular proteins. One of the tyrosine-phosphorylated proteins was identified as an Src homology-2 domain-containing protein-tyrosine phosphatase, SHP2. The stimulatory effect of the agonist peptide on early gene transcription was markedly blocked by pertussis toxin treatment whereas the induced tyrosine phosphorylation of SHP2 was completely abolished by the drug. More importantly, while expression of wild-type SHP2 enhanced the agonist-stimulatory mitogenic activity, overexpression of a catalytically inactive mutant of SHP2 strongly suppressed the stimulatory effect of the agonist peptide on both early gene transcription and DNA synthesis. These results suggest that SHP2 acts as a positive regulator linked to the PAR2-mediated mitogenic pathway coupled to a pertussis toxin-sensitive heterotrimeric G protein. Demonstration of SHP2 as a positive mediator in a G protein-coupled, receptor-mediated signaling adds to our understanding of the function of both SHP2 and PAR2 in the signaling pathway

Predictability of the Evolution of the Soil Structure using Water Flow Modeling for a Constructed Technosol

International audienceThis paper focuses on the rela on between the structure of a constructed Technosol and its hydraulic characteris cs during its early pedogenesis. The method is based on a 3-yr comparison of, on one hand, experimental measurements from an in situ gravita on lysimeter and, on the other hand, a modeling approach with HYDRUS-1D. The change of water fl ow pa erns with me was described. It was consistent with previous results for constructed Technosol aggrega on. Apart from seasonal varia ons, the specifi city of the hydraulic func oning of the constructed Technosol was shown to be due to the nature of its technogenic parent materials. The in situ evolu on of the hydrodynamics has been established and partly linked to external factors (climate, vegeta on). The direct modeling and the op miza on of the parameters over fi rst a 3-yr period and then three 9-mo periods accurately represented global water fl ow trends at the pedon scale. However it failed to simulate precisely the main events, such as massive leachate ou low. An evolu on with me of some of the hydraulic proper es was shown, expressing the structuring of the soil. The existence of two dis nct me-scales (slow and steady/fast and cyclic) of the evolu on of hydraulic parameters was then formulated as a new hypothesis. Abbrevia ons: Hz, horizon; TDR, me domain refl ectometry

Experimental Study of the Human Ability to Deliberately Excite a Flexible Floor

peer reviewedHuman-structure interactions are not completely understood yet. This paper presents an experimental study focusing on the interaction between a human and a flexible support, with a major aim to better understand how the behavior of a human subject, bouncing on a flexible structure, adapts to the support motion. Exploratory experiments are undertaken with a 7m-span timber footbridge tested in the Human Motion Analysis Laboratory of the University of Liege. The movements of the coupled system composed of the subject and the footbridge are recorded by Motion Capture technology; the subject\textquoteright{}s 3-D body motion is defined by three important angles: ankle, knee and hip. The interaction between the subject and the footbridge is also quantified by means of force platform measurements. The experiments indicate an influence of the support amplitude and natural frequency on the phase shift between the oscillatory floor motion and the three angles characterizing the motion

Relevance of adding a triangular dynamic cushion on a traditional chair: A 3D-analysis of seated schoolchildren

Background: Low back pain is highly prevalent in the general population and is even reported as early as at primary school. A poor sitting position has been suggested as an etiologic factor. This study analysed, in primary schoolchildren, the influence of a triangular dynamic cushion that aims to help children maintain their physiological lumbar lordosis and to induce movement to reduce the static effect of the sitting position. Methods: Thirty 8-year-old children took part in this study. A 3D analysis combined with electromyography was used to evaluate the biomechanics and the related muscle activation in two sitting positions (with and without a triangular cushion on a horizontal stool) during a 15-minute working task. In addition, the force of the feet on the ground was assessed with a force plate. Findings: The cushion improved the trunk–thighs angle, lumbar lordosis, anterior pelvis tilt, and feet support on the ground (p < 0.0001). In addition, sitting on the cushion appeared to be more dynamic (p < 0.05) and induced a decrease of the lumbar paravertebral muscle activity (p < 0.01). Interpretation: Sitting on a dynamic triangular cushion tends to favour the “ideal” siting position usually described in the literature and to decrease the level of paravertebral muscle recruitment. Seeing that sitting position is a risk factor to develop low back pain, the cushion could be a solution to prevent it

Uso da micropedologia na descrição de processos pedogenéticos em Tecnossolos

International audienceTechnosols are characterized by the presence of mineral and organic parent materials of technogenic origin (e.g. agricultural or urban wastes, industrial by-products, building materials, transported natural materials). In view of the continual increase of such man-made soils, there is a true need of understanding their functioning and evolution. Micropedology, i.e. morphological and analytical characterization of pedofeatures on soil sections, appears as a relevant approach to take into account the diversity and the specificity of Technosols in the knowledge of their pedogenetic processes. Micropedology was investigated at microscopic and submicroscopic scale on four Technosols. Therefore, it determined specific features of anthropogenic constituents allowing in situ monitoring until the early stages of Technosol pedogenesis. Organic matter dynamics, soil porosity evolution, impact of faunal activity or hydric conditions on Technosol structure were investigated. Moreover, as Technosol components and deposition modes are diverse, one can expect numerous interfaces. In that way, micropedology appeared particularly well adapted to study these local interfaces as sites of favoured pedogenesis. Supplemented with overall physico-chemical soil analyses, characterization of Technosol pedogenic features using micropedology improves the understanding of their functioning and evolution. In addition, according to the environmental context, such data also give useful information for the Technosol management.RESUMEN Los Tecnosoles se caracterizan por la presencia de materiales orgánicos y minerales de origen tecnogenético (p.ej. residuos agrícolas o urbanos, subproductos industriales, materiales de construcción, materiales naturales transportados, etc.). Dado el aumento continuo de estos tipos de suelos antrópicos, es necesario comprender su funcionamiento y evolución. La micropedología, definida como la caracterización morfológica y analítica de microestructuras de láminas de suelo, es una herramienta estándar para el estudio de los mismos. La micropedología ofrece un enfoque relevante para el conocimiento de los procesos edafogenéticos de los Tecnosoles, ya que permite considerar la diversidad y la especificidad de los mismos. Cuatro tipos de Tecnosoles fueron investigados mediante técnicas de micropedología a escala fotónica y de ultraestructura. Así, fue posible determinar las características de los constituyentes antropogénicos y realizar un seguimiento in situ hasta las fases tempranas de la edafogénesis de estos Tecnosoles. Se investigaron procesos como la dinámica de la materia orgánica, la evolución de la porosidad y el impacto de la actividad de la fauna o de las condiciones hídricas en la estructura de los Tecnosoles. Además, ya que los Tecnosoles son muy diversos tanto en sus componentes como en el modo en que estos componentes se organizan, es esperable que existan numerosas interfaces entre horizontes de suelo. De este modo, la micropedología se adapta muy bien al estudio de estas interfaces locales donde se dan procesos edafogenéticos. La caracterización micropedológica de las microestructuras de los Tecnosoles, complementada con análisis físico-químicos generales, incrementa de modo sustancial la comprensió