Determination of tumor-infiltrating lymphocyte density.

<p>Examples of tumor center chips with CD3 (A), CD8 (B) and FoxP3 (C) staining. Left panels are examples of high TIL densities and right panels are examples of low TIL densities. The stroma is circled in red, the epithelium in blue, and black areas are excluded. The marked areas that are taken into account to calculate the lymphocyte infiltrate density are circled in yellow. All data were acquired with Chip’s N Cheap TMA analysis program.</p

Percentages of tumor-infiltrating lymphocytes in the different areas studied.

<p>The bar charts represent the percentages of surfaces marked by CD3, CD8 and FoxP3 immunostaining in the stromal (stro) and epithelial (ep) compartments, in the tumor center (TC) and the invasion front (IF) tissues. Standard errors are given by the bars. CD3+ TIL densities were respectively, 2.2±2.5% in tumor center epithelium compartment, 6.4±5.1% in tumor center stromal compartment, 2.3±2.5% in invasion front epithelium compartment, 7.2±6.1% in invasion front stromal compartment. CD8+ TIL densities were respectively, 1.52±2.0% in tumor center epithelium compartment, 2.9±2.7% in tumor center stromal compartment, 1.8±2.4% in invasion front epithelium compartment, 3.3±3.0% in invasion front stromal compartment. FoxP3+ TIL densities were respectively, 0.13±0.12% in tumor center epithelium compartment, 0.59±0.53% in tumor center stromal compartment, 0.14±0.14% in invasion front epithelium compartment, 0.63±0.58% in invasion front stromal compartment.</p

Correlation between FoxP3+ tumor-infiltrating lymphocyte (TIL) density in stromal compartment of tumor center and clinicopathological variables.

<p>TILs: tumor-infiltrating lymphocytes, VELIPI: vascular emboli or lymphatic invasion or perineural invasion.</p

Table <b>1.</b> Patient and tumors characteristics.

<p>n: number of patients, VELIPI: vascular emboli or lymphatic invasion or perineural invasion.</p