Image 1. Jean Peccoud; photo courtesy of Ivan Morozov, Virginia Tech.

<p>Image 1. Jean Peccoud; photo courtesy of Ivan Morozov, Virginia Tech.</p

Historical distribution of synthetic biology articles published by PLOS ONE.

<p>This figure reports the number of articles in the collection published between 2006 and 2011. It shows a rapid growth of synthetic biology that reflects the growth of the journal and the increased familiarity of synthetic biologists with PLOS ONE.</p

Relationships between article-level metrics.

<p>For articles published between 2006 and 2009, there is a positive correlation between the number of times an article is cited in the scientific literature and the number of times it is viewed (A). For articles published between 2010 and 2012, there is a positive relationship between the number of views and the number of citations in the Mendeley social network (B). Metrics, such as number of views and citations in social media, give readers and authors an estimate of the scientific impact of individual articles well before they receive citations in scientific literature.</p

Positions of the primer binding sites in the internal transcribed spacer 2 (ITS2) of <i>C</i><i>. pruni</i> groups A and B.

<p>Primers are shown in black for set 1, gray for set 2 and white for set 3.</p

Origin of <i>C</i><i>. pruni</i> individuals used in this work.

<p>The 12 samples previously genotyped and assigned to groups A or B in Sauvion et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072454#B15" target="_blank">15</a>] are shown in black. The 14 samples added to cover the known distribution area of <i>C</i><i>. pruni</i> are shown in gray. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072454#tab1" target="_blank">Table 1</a> for details on each location.</p

Gene Shuffled Vesicular Stomatitis Virus Variants with Associated Statistics.

Gene Shuffled Vesicular Stomatitis Virus Variants with Associated Statistics.</p

Simplified Model of Vesicular Stomatitis Virus.

Visual representation of the simplified model used in this paper. Every molecule depicted has a degradation reaction associated with it that is not represented here.</p

N gene position most strongly influences the percentage of infections that are aborted.

The average percent of infections aborted as a function of the gene position of genes (A) N, (B) P, (C) M, (D) G, and (E) L for all 120 variants. The gene position is directly related to transcription rate of each gene. The error bars are the standard error of the mean and the orange dots represent the individual variants.</p

Simulation and Loading time of ODE Models Increases as the Number of Reactions in the Model Increase.

These two graphs show how the time to load each model (B) and simulate the model (A) increase drastically as the number of reactions in the model increases. (PDF)</p

Amplicons from PCRs using the ITS2 primer sets designed for the characterization of <i>C</i><i>. pruni</i> individuals.

<p>Panel A: typical bands obtained from single individuals belonging to groups A or B, and of pools of two individuals of each group (A+B) with each set of primers. The rightmost and leftmost lanes used a 100 bp DNA ladder. Numbers indicate the expected sizes of the amplicons. Panels B and C: PCRs using generic ITS2 primers FCM/B1D and the primer set 3 (Cp135F / CpB315R / CpA425R), respectively, on single individuals from the 24 psyllid species described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072454#tab2" target="_blank">Table 2</a>.</p