Sector-specific development and policy vulnerability in the Philippines

Ministry of Education, Singapore under its Academic Research Funding Tier

MOESM1 of Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

Additional file 1: Table S1. Plasmodium falciparum genes showing the 10 lowest female to male mRNA ratio (male specific) and the 10 highest female to male mRNA ratio (female specific), from Lasonder et al. [16]. Table S2. Primer sequences for amplification of target constructs. Table S3. Limit of Quantification and quality parameters of RTqPCR assays. Table S4. Parameters of the formula to extrapolate gametocyte sex ratio from RTqPCR data. Figure S1. Melting curves of RTqPCR assays. Figure S2. Standard Curves of RTqPCR assays. Figure S3. Expression of target genes in male and female P. falciparum gametocytes. Figure S4. Sensitivity of pfs25 Taqman and pfs25 SYBR Green assays according to RNA quality/quantity