136 research outputs found
ACC Synthase Genes Related to Cold-dependent Ripening in Pear Fruit
The differential regulation of ACC synthase genes has been studied in pear
cultivars that either require a long chilling treatment before they are capable of
ripening (‘Passe-Crassane’, PC) or not (‘Old-Home’, OH) and in OH x PC hybrids
having no (A16) or intermediate (A50) cold requirement. Among the seven Pc-ACS
cDNAs isolated, four of them (Pc-ACS1a/b and Pc-ACS2a/b) showed differential
expression in relation with cold requirement. Pc-ACS1a transcripts accumulated
specifically during chilling and ripening of cold-dependent cultivars while Pc-ACS1b
transcripts were detected only during ripening of cold-independent genotypes. Pc-
ACS2a mRNA was expressed specifically in cold-dependent genotypes and
negatively regulated by ethylene while Pc-ACS2b transcripts accumulated only in
cold-independent genotypes and positively regulated by ethylene. Pc-ACS3, 4 and 5
transcripts accumulation was similar in all genotypes, independently of coldrequirements
Mechanism of Fruit Ripening - Chapter 16
The fruit ripening process has been viewed over the last decades as being
successively of physiological, biochemical, and molecular nature. Fruit ripening
is accompanied by a number of biochemical events, including changes in color,
sugar, acidity, texture, and aroma volatiles that are crucial for the sensory quality
(Fig. 16.1). At the late stages of ripening, some senescence-related physiological
changes occur that lead to membrane deterioration and cell death. In that regard,
fruit ripening can thus be considered as the first step of a programmed cell death
process. All biochemical and physiological changes that take place during fruit
ripening are driven by the coordinated expression of fruit ripening-related genes.
These genes encode enzymes that participate directly in biochemical and physiological
changes. They also encode regulatory proteins that participate in the signaling
pathways, and in the transcriptional machinery that regulate gene expression
and set in motion the ripening developmental progra
Contribution of genomics to postharvest biology
Purpose of review: This review aims at presenting the actual and potential contribution of genomics to the understanding of the fruit ripening process and to the genetic improvement of fruit quality and storability. Findings: The advent of high throughput technologies for the sequencing of mRNAs and genomic DNA has sped up the study of gene expression and the decoding of the genome of fruit species. Genomic resources are now available that facilitate the definition of molecular markers for marker-assisted breeding, the functional identification of genes involved in fruit quality traits, the understanding of the network of events underlying the fruit ripening process and of the impact of external factors such as postharvest treatments. Directions for future research: Up to now, the development of genomic tools for studying the fruit ripening process have been carried out mostly using tomato as a model fruit. There is a need for applying genomic methods to the understanding of fruit ripening in other species, particularly non-climacteric fruit. Efforts should also be directed towards the elucidation of the function of the genes in planta and of the regulation of their expression. So far, among the several hundreds of genes whose expression is altered during ripening, very few have well characterized functions. The number of genes for which a picture of the regulatory events is available is extremely limited
A reliable system for the transformation of cantaloupe charentais melon (Cucumis melo L. var. cantalupensis) leading to a majority of diploid regenerants
An efficient system of transformation leading to a majority of transformed diploid plants from
leaf explants of Cucumis melo L. var. Cantalupensis (cv. Védrantais) was developed. Several
regeneration protocols using cotyledon or leaf explants were analysed with particular emphasis on
the regeneration efficiency and the ploidy level of the regenerated melon plants. The use of leaf
explants excised from 10 day-old seedlings, cultured in Murashige and Skoog's medium
supplemented with 1 mM 6-benzylaminopurine (BAP) and 1 mM 6-(g,g-dimethylallylamino)-purine
(2iP), resulted in a high regeneration frequency (73%). In these conditions, more than 84% of the
regenerated plants were found to be diploid. Addition of an Agrobacterium-mediated transformation
step did not significantly change the percentage (81.8%) of diploid plants regenerated. This
protocol was successfully used to produce diploid transgenic melon plants expressing the antisense
ACC oxidase gene, encoding ACC oxidase which catalyses the last step of ethylene biosynthesis.
Ethylene production and ACC oxidase activity of the leaf explants from transgenic plants was
reduced by more than 80% as compared to the control untransformed tissues. This transformation/
regeneration method could be routinely used for the introduction of other genes of interest in melon
Regulation of tomato fruit ripening
Fruit ripening is a sophisticatedly orchestrated developmental process, unique to plants, that
results in major physiological and metabolic changes, ultimately leading to fruit decay and seed
dispersal. Because of their strong impact on fruit nutritional and sensory qualities, the ripeningassociated
changes have been a matter of sustained investigation aiming at unravelling the
molecular and genetic basis of fruit ripening. Tomato rapidly emerged as the model of choice for
fleshy fruit research and a wealth of genetic resources and genomics tools have been developed,
providing new entries into the regulatory mechanisms involved in the triggering and coordination
of the ripening process. Some of the key components participating in the control of tomato fruit
ripening have been uncovered, but our knowledge of the network of signalling pathways engaged in
this complex developmental process remains fragmentary. This review highlights the main
advances and emphasizes issues still to be addressed using the rapidly developing ‘omics’
approaches
Potential for Ethanol Vapours to Limit Table Grape Berry Shatter and to Limit Ethylene Evolution from Clusters
We have shown previously that ethanol vapours (given by 2 ml per kg of grapes) can prevent Botrytis development and stem browning, two of the major problems in postharvest quality of table grapes. In the present paper, we will give emphasis to preliminary results about (i) the role of ethanol vapours in the inhibition of berry shatter and (ii) the control of ethylene evolution from grapes bunches by ethanol vapours and the link to the control of Botryti
ER5, a tomato cDNA encoding an ethylene-responsive LEA-like protein: characterization and expression in response to drought, ABA and wounding
We report the isolation by differential display of a novel tomato ethylene-responsive cDNA, designated ER5.
RT-PCR analysis of ER5 expression revealed an early (15 min) and transient induction by ethylene in tomato fruit,
leaves and roots. ER5 mRNA accumulated during 2 h of ethylene treatment and thereafter underwent a dramatic
decline leading to undetectable expression after 5 h of treatment. The full-length cDNA clone of 748 bp was
obtained and DNA sequence analysis showed strong homologies to members of the atypical hydrophobic group of
the LEA protein family. The predicted amino acid sequence shows 67%, 64%, 64%, and 61%sequence identity with
the tomato Lemmi9, soybean D95-4, cotton Lea14-A, and resurrection plant pcC27-45 gene products, respectively.
As with the other members of this group, ER5 encodes a predominantly hydrophobic protein. Prolonged drought
stress stimulates ER5 expression in leaves and roots, while ABA induction of this ethylene-responsive clone is
confined to the leaves. The use of 1-MCP, an inhibitor of ethylene action, indicates that the drought induction of
ER5 is ethylene-mediated in tomato roots. Finally, wounding stimulates ER5 mRNA accumulation in leaves and
roots. Among the Lea gene family this novel clone is the first to display an ethylene-regulated expression
Molecular Control of Fruit Ripening and Sensory Quality of Charentais Melon
Traditional Charentais melons have a typical climacteric behavior with
ethylene playing a major role in the regulation of the ripening process. Genetic studies
using climacteric and non-climacteric types of Cucumis melo demonstrated that the
climacteric character is dominant and conferred by 2 duplicated loci only which are of
great importance for the regulation of storability and sensory quality. Commercial
varieties of Charentais melon with long shelf-life have been generated, some of them
by crossing with a non-ripening Charentais genotype (Vauclusien). The introduction
of the long shelf-life character resulted in undesirable loss of aroma volatiles
production. The inhibition of ethylene synthesis by knocking-down ACC oxidase gene
expression has been achieved in Charentais melon. It results is a strong inhibition of
the synthesis of aroma volatiles while the accumulation of sugars is not affected or is
even improved and the softening of the flesh is strongly affected but not abolished. It
was also demonstrated that ethylene-inhibited fruit exhibited better resistance to
chilling injury. Due to the importance of aroma volatiles in sensory quality and to the
strong negative correlation between aroma production and ethylene synthesis, we have
developed a research program aimed at isolating genes involved in the synthesis of
volatile esters, compounds that are essential for the flavor of Cantaloupe melons. We
report here on the recent advances in the field with special emphasis on the characterization
of two families of genes encoding aldehyde reductases and alcohol acyl
transferases
Characterization of Genes Involved in the Formation of Aroma Volatiles in "Charentais" Melon Fruit
Volatiles esters impart distinct characteristics to the fruit quality.
"Charentais" cantaloupe melon (Cucumis melo "cantalupensis") is characterized by
abundant sweetness and aromatic flavour. Plant alcohol acyl transferase (AAT)
genes have been identified and shown to be involved in aromas production. Recently,two cDNAs (Cm-AAT1 and Cm-AAT2) putatively involved in the formation of aroma volatile esters have been isolated from melon fruit. Cm-AAT1 protein exhibit alcohol acyl transferase activity while no such activity could be detected for Cm-AAT2. Two new cDNAs (Cm-AAT3 and Cm-AAT4) have been isolated from melon fruit that
showed 69% and 36% similarity, respectively, with Cm-AAT1. The percentage
similarity over the whole amino acid sequence between them is 34%. Cm-AAT3 and
Cm-AAT4 show the highest similarity to the tobacco Nt-HSR201 protein and a rose
alcohol acyltransferase Rh-AAT1, respectively. All Cm-AATs genes, share three
conserved regions common to the BAHD acyltransferase gene superfamily.
Heterologous expression in yeast revealed that some of the encoded proteins have a
wide range of specificity while others are specific to a narrow range of substrates
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