<b>Estimated genetic correlations extracted from the fitted multi-environment model for individual traits.</b>

<p><b>Estimated genetic correlations extracted from the fitted multi-environment model for individual traits.</b></p

Location of Cl⁻ concentration QTL (<i>barc56</i>/<i>gwm186</i>) on chromosome 5A detected in field trials (Balaklava, Georgetown and Roseworthy) with varying salinity levels.

<p>The outlier statistics represent LOD scores (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098845#pone-0098845-t004" target="_blank">Table 4</a>).</p

<b>Candidate genes underlying the physical interval of the Cl⁻ QTL on chromosome 5A.</b>

a<p>according to MSU Rice Genome Annotation Project release 7, Ensembl Plants release 22 or NCBI;</p>b<p>Genome Zipper v5.</p

<b>QTL associated with Ca²⁺ and Mg²⁺ concentrations in hydroponics and in a field trial (Balaklava) under varying degrees of salinity stress.</b>

<p>Only those intervals with <i>P</i> values ≤0.01 and LOD>2.0 are presented. Balaklava location was classified as moderate salinity. Please see Genc et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098845#pone.0098845-Genc2" target="_blank">[24]</a> for soil salinity classification.</p><p>*Positive and negative values indicate that Berkut and Krichauff alleles increased the phenotypic values, respectively. QTL names with letter C indicate several co-locating markers at those loci.</p

<b>Estimated genetic correlations between shoot DW (hydroponics), grain yield (field) Na⁺, K⁺, and Cl⁻ (field and hydroponics) extracted from the fitted multi-trait model at each environment.</b>

<p><b>Estimated genetic correlations between shoot DW (hydroponics), grain yield (field) Na⁺, K⁺, and Cl⁻ (field and hydroponics) extracted from the fitted multi-trait model at each environment.</b></p

<b>Parental means, population mean and range for Na⁺, Cl⁻, K⁺, Ca²⁺ and Mg²⁺ concentrations (mmol kg¹ DW) in penultimate leaves (field trials) and whole shoots (hydroponics), seedling biomass [shoot DW (g plant¹)] and grain yield (t ha¹) in Berkut/Krichauff DH population tested for ST in hydroponics and field trials (Roseworthy, Balaklava and Georgetown).</b>

<p>The means represent predicted values from MET (Na⁺, Cl⁻, K⁺) and single environment (Ca²⁺ and Mg²⁺) analysis of each trait. Broad-sense heritability is also given for individual traits at each environment.</p

Additional file 7: Table S4. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Breeding origin, genotype at HvBot1, and B tolerance phenotype of 80 current or recent Australian barley varieties grown to maturity in a glasshouse with an elevated supply of B. Breeding lines Parent 19 and Ethiopia 756 were also included in the screen, as well as Sahara 3771 and Clipper control genotypes. All varieties had a Clipper (B-intolerant) allele at chromosomes 6H and 3H, while at 2H only Sloop Vic_A, Sloop Vic_B and Ethiopia 756 possessed the tolerant (Sahara) allele. Varieties are listed in order of increasing severity of leaf symptoms expression. Symptoms were assessed visually three times during the growth of plants to full maturity and an average score determined (0 = no necrosis; 6 = severe necrosis). The penultimate leaves from five tillers were sampled at mid-grain fill for determination of leaf B concentration (PDF 464 kb

Additional file 8: Table S5. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Barley genotypes included in each (a) HvBot1 allele class and (b) HvNIP2;1 haplotype class, for gene expression analyses data presented in Figs. 1b and 3b, respectively (PDF 301 kb

Additional file 5: Table S3. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Primers, PCR markers and probes used in this study (PDF 350 kb

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19 août 18801880/08/19 (A14,N5217)