3 research outputs found
Total Synthesis and Functional Evaluation of IORs, Sulfonolipidâbased Inhibitors of Cell Differentiation in Salpingoeca rosetta
The choanoflagellate Salpingoeca rosetta is an important model system to study the evolution of multicellularity. In this study we developed a new, modular, and scalable synthesis of sulfonolipid IORâ1A (six steps, 27â% overall yield), which acts as bacterial inhibitor of rosette formation in S.â
rosetta . The synthesis features a decarboxylative crossâcoupling reaction of a sulfonic acidâcontaining tartaric acid derivative with alkyl zinc reagents. Synthesis of 15 modified IORâ1A derivatives, including fluorescent and photoaffinityâbased probes, allowed quantification of IORâ1A, localization studies within S.â
rosetta cells, and evaluation of structureâactivity relations. In a proof of concept study, an inhibitory bifunctional probe was employed in proteomic profiling studies, which allowed to deduce binding partners in bacteria and S.â
rosetta . These results showcase the power of synthetic chemistry to decipher the biochemical basis of cell differentiation processes within S.â
rosetta
Complex extracellular biology drives surface competition during colony expansion in <i>Bacillus subtilis</i>
Recent highlights of biosynthetic studies on marine natural products
Marine bacteria are excellent yet often underexplored sources of structurally unique bioactive natural products. In this review we cover the diversity of marine bacterial biomolecules and highlight recent studies on structurally novel natural products. We include different compound classes and discuss the latest progress related to their biosynthetic pathway analysis and engineering: examples range from fatty acids over terpenes to PKS, NRPS and hybrid PKS-NRPS biomolecules. This journal is </p