Pathologie des infections par les morbillivirus chez les mammifères marins

Morbillivirus infections were described since 1988 in marine mammals including pinnipeds and cetaceans. Since, numerous outbreaks, responsible for thousands marine mammals death, as well as sporadic cases, have occurred in various populations from North Atlantic, Mediterranean Sea, Baikal Lake, Caspian Sea, and recently from northern Pacific. Clinical signs and macroscopic lesions are similar between species infected by morbillivirus and are close to those reported in dog with canine distemper. Under microscope, characteristic les ions are interstitial bronchopneumonia, lymphoid depletion and subacute encephalitis. Syncytia and inclusion bodies are frequently observed in lesions and epithelia. Lymphoid depletion promotes secondary infections by opportunistic pathogens, responsible in many cases of the animals death. Various parameters explain the high sensibility of certain population such as the species, the naive immunological status and vectors intervention for the disease

Hepcidin and iron species distribution inside the first-trimester human gestational sac

We have investigated factors affecting iron distribution in the first-trimester gestational sac, by the measurement of transferrin, non-transferrin-bound iron (NTBI) and pro-hepcidin (Hep) in maternal serum, coelomic fluid (CF) and amniotic fluid (AF) and by immunostaining for Hep in villous and secondary yolk sac biopsies. These samples were obtained from 15 first-trimester pregnancies at 8–11 weeks gestation. Transferrin concentrations were significantly lower in fetal (0.56 mg/ml) than maternal serum (1.71 mg/ml), with very low concentrations in CF and AF (0.09 mg/ml). In contrast, transferrin saturations were significantly higher in fetal (77%) than maternal serum (33%). NTBI was present in fetal serum, CF and AF, presumably as a consequence of low transferrin concentrations in these compartments. Pro-Hep was present at lower levels in fetal (140.0 ± 11.1) than maternal serum (206.2 ± 9.2) and at low concentrations in CF (19.4 ± 3.1) and AF (21.8 ± 5.2). Immunostaining with Hep antibody was found in the syncytiotrophoblast of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac at 10 weeks. The presence of Hep in syncytiotrophoblast cells of first-trimester placenta as well as in mesothelial and endodermal layers of the secondary yolk sac suggest a key regulatory role for this protein in iron transfer to the first-trimester fetus. The low transferrin concentrations and the presence of NTBI in CF and AF suggest that transferrin-independent iron transfer is important in early gestation

Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises <i>Phocoena phocoena</i> from the southern North Sea

Background: Harbour porpoises Phocoena phocoena from the southern North Sea are known to display high levels of Zn and Hg in their tissues linked to their nutritional status (emaciation). The question arises regarding a potential role of metallothioneins (MTs) with regard to these high metal levels. In the present study, metallothionein detection and associated Zn, Cd, Cu and Hg concentrations were investigated in the liver and kidney of 14 harbour porpoises collected along the Belgian coast. Results: Metallothioneins seemed to play a key role in essential metal homeostasis, as they were shown to bind 50% of the total hepatic Zn and 36% of the total hepatic Cu concentrations. Renal MTs also participated in Cd detoxification, as they were shown to bind 56% of the total renal Cd. Hg was mainly found in the insoluble fraction of both liver and kidney. Concomitant increases in total Zn concentration and Zn bound to MTs were observed in the liver, whereas Zn concentration bound to high molecular weight proteins remained constant. Cu, Zn and Cd were accumulated preferentially in the MT fraction and their content in this fraction increased with the amount in the hepatocytosol. Conclusion: MTs have a key role in Zn and Cu homeostasis in harbour porpoises. We demonstrated that increasing hepatic Zn concentration led to an increase in Zn linked to MTs, suggesting that these small proteins take over the Zn overload linked to the poor body condition of debilitated harbour porpoises

The diet of harbour porpoises bycaught or washed ashore in Belgium, and relationship with relevant data from the strandings database

The harbour porpoise Phocoena phocoena is currently the most abundant marine mammal in Belgian waters. Large-scale changes in the distribution of this top predator in the North Sea have occurred during the last decades, possibly caused by changes in food availability. An analysis of the strandings database 1970-2011, containing data on 737 harbour porpoises, revealed that throughout the year two peaks in strandings occurred: one in spring, for an important part caused by the strandings of animals incidentally bycaught in fishing gear, and a second one during summer. Most of the stranded animals were juveniles, with a higher percentage of males than females. As could be expected, bycaught animals were on average heavier than equally sized naturally died animals, and they had a thicker blubber layer. In naturally died animals the blubber layer was thicker in winter than in summer, illustrating the insulating function of the blubber layer besides its function as an energy storage.We investigated the stomach content of 64 harbour porpoises washed ashore or bycaught in Belgium between 1997 and 2011. Ten of the stomachs were empty. Fish contributed to most of the prey remains. In total we found the remains of 19 fish species belonging to 10 families. The numerically most important prey items in juveniles were gobies (Gobiidae). Reconstructing the original weight of the prey items revealed that gobies constituted the most important prey by weight, but that larger sandeels (Ammodytidae) and to a lesser extent gadoids (Gadidae) were also important. In adults the majority of prey items were gobies and sandeels, but the reconstructed weight of the stomach content revealed that sandeels and gadoids constituted by far the most important prey. Surprisingly, clupeids (Clupeidae) did not contribute much to the diet, although the return of the harbour porpoise is often linked to an increase in herring Clupea harengu stocks in the southern North Sea. Also no twaite shad Alosa fallax were found, although this diadromic fish is common again in the area. A small number of smelt Osmerus eperlanus was found in some of the recently stranded harbour porpoises. Smelt is a diadromic fish of which densities are increasing from very low levels. The fact that juvenile harbour porpoises had apparently fed on large quantities of small bottom fish may help to explain why they were more prone to bycatch than adults. The analysis revealed that a gradual shift occurs in the feeding habits of harbour porpoises while becoming adults: from small benthic fish towards larger fish taken from the water column