PLoS ONE

Lyme disease is a multisystemic disorder caused by B. burgdorferi sl. The molecular basis for specific organ involvement is poorly understood. The skin plays a central role in the development of Lyme disease as the entry site of B. burgdorferi in which specific clones are selected before dissemination. We compared the skin inflammatory response (antimicrobial peptides, cytokines and chemokines) elicited by spirochete populations recovered from patients presenting different clinical manifestations. Remarkably, these spirochete populations induced different inflammatory profiles in the skin of C3H/HeN mice. As spirochete population transmitted into the host skin is heterogeneous, we isolated one bacterial clone from a population recovered from a patient with neuroborreliosis and compared its virulence to the parental population. This clone elicited a strong cutaneous inflammatory response characterized by MCP-1, IL-6 and antimicrobial peptides induction. Mass spectrometry of this clone revealed 110 overexpressed proteins when compared with the parental population. We further focused on the expression of nine bacterial surface proteins. bb0347 coding for a protein that interacts with host fibronectin, allowing bacterial adhesion to vascular endothelium and extracellular matrix, was found to be induced in host skin with another gene bb0213 coding for a hypothetical protein. These findings demonstrate the heterogeneity of the B. burgdorferi ss population and the complexity of the interaction involved early in the skin

Microarray Analyses of Inflammation Response of Human Dermal Fibroblasts to Different Strains of Borrelia burgdorferi Sensu Stricto

In Lyme borreliosis, the skin is the key site of bacterial inoculation by the infected tick, and of cutaneous manifestations, erythema migrans and acrodermatitis chronica atrophicans. We explored the role of fibroblasts, the resident cells of the dermis, in the development of the disease. Using microarray experiments, we compared the inflammation of fibroblasts induced by three strains of Borrelia burgdorferi sensu stricto isolated from different environments and stages of Lyme disease: N40 (tick), Pbre (erythema migrans) and 1408 (acrodermatitis chronica atrophicans). The three strains exhibited a similar profile of inflammation with strong induction of chemokines (CXCL1 and IL-8) and IL-6 cytokine mainly involved in the chemoattraction of immune cells. Molecules such as TNF-alpha and NF-κB factors, metalloproteinases (MMP-1, -3 and -12) and superoxide dismutase (SOD2), also described in inflammatory and cellular events, were up-regulated. In addition, we showed that tick salivary gland extracts induce a cytotoxic effect on fibroblasts and that OspC, essential in the transmission of Borrelia to the vertebrate host, was not responsible for the secretion of inflammatory molecules by fibroblasts. Tick saliva components could facilitate the early transmission of the disease to the site of injury creating a feeding pit. Later in the development of the disease, Borrelia would intensively multiply in the skin and further disseminate to distant organs

Mise au point d'une technique de confirmation par PCR en temps réel pour la détection de borrelia burgdorferi sensu lato

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Détection de la résistance à la méticilline et identification d'espèces de staphylocoques sur des hémocultures par PCR en temps réel

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Réception de Monsieur Benoit Jaulhac le 03 Octobre 2019

Philippon Alain, Jaulhac Benoît. Réception de Monsieur Benoit Jaulhac le 03 Octobre 2019. In: Bulletin de l'Académie Vétérinaire de France tome 172, 2019. pp. 178-180

Menace d'accouchement prématuré, rupture prématurée des membranes et infections urinaires et vaginales (étude rétrospective en 2009 à l'hôpital de Hautepierre de Strasbourg)

STRASBOURG-Medecine (674822101) / SudocSudocFranceF

SEROLOGIE DE LA BORRELIOSE DE LYME PAR IMMUNO-EMPREINTE (INFLUENCE DE LA SOUCHE UTILISEE COMME ANTIGENE (DES BIOLOGIE MEDICALE))

STRASBOURG-Medecine (674822101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Détermination des espèces de borrelia burgdorferi responsables d'érythème migrant en Alsace par une technique de typage moléculaire par PCR en temps réel.

STRASBOURG-Medecine (674822101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Inflammation cutanée et borréliose de Lyme (étude in vitro des interactions entre les cellules résidentes de la peau et Borrelia)

Nous avons étudié le rôle de l'immunité innée de la peau lors de la transmission des Borrelia (agent infectieux de la borréliose de Lyme) par son vecteur, une tique dure du genre Ixodes. Nous avons montré que la salive de tique et la protéine salivaire Salp15 inhibent la réaction inflammatoire (production de chimiokines et de peptides antimicrobiens) des kératinocytes induite par Borrelia. Cet effet anti- alarmine de la salive de tique contribue probablement à créer un environnement cutané local favorable à la transmission de Borrelia. Nous avons montré que Borrelia induit également au niveau des fibroblastes cutanés la transcription de nombreux gènes proinflammatoires. Nous avons observé un effet toxique direct de la salive de tique sur les fibroblastes cutanés : cet effet dose-dépendant est de nature protéique mais non lié à la protéine Salp15. Ces résultats indiquent que les fibroblastes jouent un rôle important dans l inflammation cutanée induite par Borrelia.We studied the role of the skin innate immunity during the transmission of Borrelia (the infectious agent of Lyme borreliosis) by its vector, a hard tick belonging to the genus Ixodes. We showed that tick saliva and its protein Salp15 both inhibate Borrelia-induced inflammatory reaction of keratinocytes. The antialarmin effect of tick saliva ensure a favorable environment for Borrelia. We also showed that Borrelia induce a strong inflammatory response in dermal fibroblasts. We also demonstrate a dose-dependent lytic effect of tick salivary gland extracts on dermal fibroblasts and that this cytotoxic effect was of proteinaceous nature and not related to Salp15. These results indicate that dermal fibroblasts could be considered as central mediators in immune cell recruitment to the skin site of Borrelia invasion.STRASBOURG-Bib.electronique 063 (674829902) / SudocSudocFranceF