List of primers used in mapping studies.

<p>List of primers used in mapping studies.</p

Methylation differences between wheat varieties.

<p>DNA extracted from the embryos of mature seeds was digested with <i>Msp</i> I restriction enzyme and probed with 5S rDNA. PHS resistant varieties RL4137, Snowbird, AC Domain, AC Karma show reduced methylation as compared to a PHS susceptible variety AC Andrew. DNA methylation in CDC Teal, a variety with medium PHS reaction was also less, compared to AC Andrew but more than the PHS resistant varieties.</p

Comparing wheat homoeologs.

<p>DNA sequence comparison of wheat homoeologs of <i>AGO802</i> and <i>AGO804</i>. The three wheat <i>AGO802</i> homoeologs are almost 100% identical for the sequence and <i>AGO804</i> has sequence homology of about 98% among its three homoeologs.</p

Retroposon insertion in the <i>AGO802B</i> gene, in 10 wheat accession with varying reaction to pre-harvest sprouting.

<p>Retroposon insertion in the <i>AGO802B</i> gene, in 10 wheat accession with varying reaction to pre-harvest sprouting.</p

Phylogenetic comparison of ARGONAUTE4_9.

<p>Comparison of ARGONAUTE4_9 class of genes using a Phylogenetic tree from wheat, barley, rice, maize, sorghum and Arabidopsis.</p

Analysis of a retro-transposon insertion in the <i>AGO802B</i> transcripts.

<p>Polymorphism as observed on agarose gel in different wheat varieties with varying reaction to pre-harvest sprouting, due to insertion of a retro-transposon insertion in the <i>AGO802B</i> transcripts.</p

Expression analysis of <i>AGO802</i> and <i>AGO804</i>.

<p>Expression of <i>AGO802</i> and <i>AGO804</i> homoeologs in embryos during seed development in Chinese Spring cultivar. QRT-expression analysis was performed at six different stages consisting of ovaries at 5 DAP stages and embryos at 10, 15, 20, 25 and 30 DAP, shown on X-axis of graph as 1–6 respectively.</p

Amino acid sequence alignment of wheat CESA proteins.

<p>Drawn to scale with solid lines representing conserved amino acid sequences and the gaps representing the mismatches and deletions. Corresponding phases of intron evolution (0, 1, 2) for the CESA proteins are shown on the top of the solid lines. Primary and secondary cell wall CESAs are shown in blue and red color, respectively.</p

Unrooted phylogenetic tree of the CESAs from <i>Triticum aestivum</i> (TaCESA), <i>Arabidopsis thaliana</i> (AtCESA), <i>Beta vulgaris</i> (BvCESA), <i>Eucalyptus grandis</i> (EgCESA), <i>Glycine max</i> (GmCESA), <i>Gossypium hirsutum</i> (GhCESA), <i>Hordeum vulgare</i> (HvCESA), <i>Oryza sativa</i> (OsCESA), <i>Populus trichocarpa</i> (PtCESA), <i>Solanum tuberosum</i> (StCESA) and <i>Zea mays</i> (ZmCESA).

<p>The bar provides a scale for the branch length in the horizonal dimension. The line segment with the number '0.1' means that an equal length of the branch between the CESA proteins represents a change of 0.1 AA. Color codes for different species: Red—TaCESA, blue–AtCESA, purple—HvCESA, yellow—ZmCESA, green—OsCESA, and grey–BvCESA, EgCESA, GmCESA, GhCESA, PtCESA, StCESA.</p

Le Courrier

20 août 18421842/08/20 (N232)