Comparison of BUSCO results of the <i>D</i>. <i>rosae</i> sequence and other fungi.

<p>Comparison of BUSCO results of the <i>D</i>. <i>rosae</i> sequence and other fungi.</p

Venn diagram of expressed genes during early stages of the infection process (0, 24, 72 hpi).

<p>Three independent inoculation experiments were performed and genes are considered to be expressed at a time point, if they show expression in at least two of the three experiments. MACE and RNA-Seq data for 72 hpi were generated from the same samples.</p

Comparison of the percentage of Blastp matches with different numbers of matches to the same reference sequence between <i>D</i>. <i>rosae</i> and <i>B</i>. <i>cinerea</i>.

<p>The portion of the <i>M</i>. <i>Brunnea</i> proteome that had no second match in a Blast (E-value cutoff e-100) of the proteome against itself was the reference for the Blastp search. The cutoff for the Blastp of the related species was set to e-100. The different colors refer to the number of Blast matches to the same <i>M</i>. <i>Brunnea</i> reference sequence.</p

PCR-RFLP results of six pairs of paralogs tested with six different <i>D</i>. <i>rosae</i> isolates.

<p>PCR-RFLP results of six pairs of paralogs tested with six different <i>D</i>. <i>rosae</i> isolates.</p

k-mer distribution based on the small insert Illumina library.

<p>The plot displays the number of k-mers (size 17) generated from the reads (y-axis) that occur with a given multiplicity (x-axis). The peak represents the mean k-mer depth. K-mers with extremely low frequency (<5) are not displayed and are considered to contain sequencing errors.</p

Sequencing statistics.

<p>Sequencing statistics.</p

PCR-RFLP analysis of pairs of BUSCO orthologs.

<p>A: Schematic display of the method. Specific primers were developed that distinguish between the two very similar paralogs. As second distinctive feature only one of the two PCR fragments contains an EcoRI or DraI restriction site. B: Gel image of a PCR-RFPL analysis of the paralogous sequences DR002900 and DR003069 with six different isolates. Ladder: 1 KB plus (Thermo Fisher, Waltham, USA).</p

Identity values of <i>D</i>. <i>rosae</i> paralogs.

<p>Histogram of identity scores generated by global alignment of all duplicated BUSCO orthologs in the <i>D</i>. <i>rosae</i> assembly. The gene alignments include intron sequences.</p

SSR content of the genome.

<p>SSR content of the genome.</p

Key data of the <i>D</i>. <i>rosae</i> assembly.

<p>Key data of the <i>D</i>. <i>rosae</i> assembly.</p