23 research outputs found
Expression of Nectin-4 protein in luminal B (HER2 negative) breast cancer
Cilj istraživanja: Istražiti povezanost ekspresije Nectin-4 proteina u populaciji pacijenata s luminalnim B HER2 negativnim podtipom karcinoma dojke a u korelaciji sa prognostiÄkim parmetrima kao Å”to su intenzitet hormonskih receptora, Ki-67 proliferacijski indeks, dob, veliÄiina i histoloÅ”ki gradus tumora te zahvaÄenost pazuÅ”nih limfnih Ävorova. Materijal i metode: Ispitivana je skupina od 147 pacijenata operiranih u KBC Osijek sa unilateralnim karcinomom dojke bez dokazanih udaljenih metastaza, koji se prema najnovijoj St. Gallenskoj klasifikaciji svrstavaju u luminalnu B HER2 negativnu podskupinu. KoriÅ”tenjem arhivskih parafinskih kocaka uÄinjeno je imunohistokemijsko bojanje antitijelom na Nektin-4 protein, a rezultati su korelirani sa kliniÄkim podatcima uz Kaplan-Meier krivulje te Cox-ovu regresijsku analizu. Rezultati: Dokazana je statistiÄki znaÄajna povezanost ekspresije Nektina-4 sa veliÄinom tumora (p<0.05; Chi-Square Test) te negativna povezanost ukupnim preživljenjem, prežvljenjem bez bolesti, te preživljenjem bez pojave udaljenih metastaza (p<0,001; Kaplan-Meier). U Cox-ovoj univarijantnoj i multivarijantnoj analizi pokazao se kao statistiÄki znaÄajan nezavisni faktor za sveukupno preživljenje i preživljenje bez povrata bolesti (p<0,001). Nije uoÄena statistiÄki znaÄajna povezanost ekspresije Nektina-4 sa dobi, ekspresijom estrogenskih i progesteronskih receptora, zahvaÄenoÅ”Äu pazuÅ”nih limfnih Ävorova, diferencijacijom tumora, histoloÅ”kim tipom i Ki-67 proliferacijskim indeksom. ZakljuÄak: Nectin-4 je važan prognostiÄki pokazatelj te potencijalna meta za nove terapijske metode u lijeÄenju luminalnih B HER2 negativnih karcinoma dojke.Objectives: The aim of this study was to evaluate an expression of Nectin-4 in luminal B (HER2 negative) breast cancer, in correlation with other prognostic parameters as hormone positivity, Ki-67 proliferation index, age, tumor size, histological grade and axillary lymph node status. Patients and methods: In this retrospective study clinical and pathological parameters of 147 luminal B (HER2 negative) breast cancers have been examined from the patients who had undergone a surgery at the University Hospital Osijek. These patients were presenting with primary unilateral breast cancer with no evidence of distant metastasis. Nectin-4 protein expression was assessed by immunohistochemistry and the results were correlated with the clinical data using Kaplan-Meier curves and univariante and multivariate stepwise proportional-hazard analysis (Cox model). Results: Nectin-4 overexpression was significantly correlated with tumor size (p<0.05; Chi- Square Test), also Nectin-4 expression was negatively associated with overall survival, disease free survival and distant relapse free survival with the same significance (p<0,001; Kaplan-Meier). Cox analysis has shown statistically significant correlation between Nectin-4 and overall and disease free survival (p<0,001). Statistically significant correlation between Nectin-4 and age, ER, PR, age, lymph node metastasis, tumor differentiation, histological subtype and Ki-67 proliferation index was not found. Conclusion: Nectin-4 is relevant prognostic factor and a therapeutic target in luminal B (HER2 negative) breast cancer
Pojavnost intramiokardijalnih masnih stanica u stijenci desne pretklijetke i desne klijetke ā postmortalna humana analiza
Histologic and radiologic studies describe intramyocardial fat tissue as a normal finding or as part of cardiac pathology. The role of fat cells within the myocardium is not fully understood. The aim of this study was to assess fat tissue distribution in the myocardium of right atrium (RA) and right ventricle (RV) and age differences in subjects free from cardiac disease. The study included 10 males without cardiac disease divided into two groups according to age (below/above 50 years). Three cross sections were performed (RV free wall and apex and RA free wall) with histomorphological analysis on digital photographs. The shares of total myocardial fat (TMF), perivascular fat (PVF) and non-perivascular (nPVF) fat were calculated. Samples from the older group had larger amounts of fat in the epicardium and myocardium, without statistically significant difference (TMF p=0.847, PVF p=0.4 and nPVF p=0.4). The largest quantities of fat tissue were found in the RV apex samples (14.9%), followed by RV free wall (7.5%) and RA (4.5%), where total apical RV fat share was significantly larger than in RA sample (p=0.044). Intramyocardial fat cells were present within the non-diseased RA and RV in all samples, mostly in the apex. Further investigations on age difference, effect of visceral obesity and sex differences are needed.DosadaÅ”nja histoloÅ”ka i radioloÅ”ka istraživanja opisuju pojavnost intramiokardijalnog masnog tkiva kao dio normalnog nalaza ili kao dio patoloÅ”kog nalaza srca. Uloga intramiokardijalnih masnih stanica nije joÅ” razjaÅ”njena. Cilj ovoga istraživanja bio je utvrditi raspodjelu masnog tkiva u miokardu desne pretklijetke (DP) i desne klijetke (DK) ovisno o dobi i lokalizaciji kod ispitanika bez kardijalnog morbiditeta. PatohistoloÅ”ka postmortalna studija ukljuÄila je 10 muÅ”karaca bez kardijalnih bolesti podijeljenih u dvije skupine prema dobi (ispod/iznad 50 godina). Uzorci su uzeti u tri presjeka (projekcija slobodnog zida DK, slobodni zid vrha DK te stijenka DP) s histomorfoloÅ”kom analizom na digitalnim fotografijama. IzraÄunati su udjeli ukupnog masnog tkiva unutar srÄanog miÅ”iÄa (UMT), perivaskularnog masnog tkiva (PMT) i ne-perivaskularnog masnog tkiva (nPMT). U uzorcima starijih ispitanika prisutna je veÄa koliÄina masnog tkiva u epikardu i miokardu, bez statistiÄki znaÄajne razlike (UMT p=0,847, PMT p=0,4, nPMT p=0,4). NajveÄe koliÄine masnog tkiva pronaÄene su u uzorcima vrha DK (14,9%), nakon Äega slijedi stijenka slobodnog zida DK (7,5%) i DP (4,5%), gdje je ukupni udio masnog tkiva DK bio znatno veÄi nego kod uzorka DP (p=0,044). Intramiokardijalne masne stanice sastavni su dio histoloÅ”kog nalaza DP i DK u svim uzorcima bez prisutnih kardijalnih bolesti, dominantno u vrhu DK. Neophodna su daljnja istraživanja utjecaja dobi, spola i visceralne pretilosti na pojavnost intramiokardijalnih masnih stanica unutar stijenke desnog srca
Udio turmoske strome kao loÅ” prognostiÄki Äimbenik kod karcinoma debelog crijeva
High stroma proportion appears to be a very important prognostic factor in esophageal and breast cancer. Previous researches have shown that it might have a similar effect on colorectal cancer. The aim of this study was to determine whether tumor stroma proportion influenced patient survival. This retrospective study included 236 patients with colorectal cancer having undergone surgery in 2006 and 2007 at Osijek University Hospital Center. Location with the highest stroma proportion at the site of deepest tumor invasion was determined. Patients were divided into the groups with high stroma proportion (>50%) and low stroma proportion (ā¤50%). Stroma proportion showed a statistically significant correlation with tumor stage. Kaplan-Meier survival curves yielded a statistically significant difference in patient overall survival (Cox ph model p=0.016) and progressionfree survival (Cox ph model, p=0.0188) according to stroma proportion. Study results showed a statistically significantly shorter overall survival and progression-free survival in patients with high stroma proportion.Dokazano je da je visok udio strome znaÄajan prognostiÄki Äimbenik kod karcinoma jednjaka i karcinoma dojke. Prethodna istraživanja su pokazala da bi isti utjecaj stroma mogla imati i kod kolorektalnog karcinoma. Cilj istraživanja je bio utvrditi je li udio tumorske strome povezan s preživljenjem bolesnika s kolorektalnim karcinomom. Provedeno je retrospektivno istraživanje u 236 bolesnika koji su operirani na Klinici za kirurgiju KliniÄkoga bolniÄkog centra Osijek. OdreÄeno je podruÄje s najveÄim udjelom strome u podruÄju najdubljeg prodora tumora. Bolesnici su podijeljeni u dvije skupine: onu s visokim udjelom strome (>50%) i onu s niskim udjelom strome (ā¤50%). Udio tumorske strome bio je statistiÄki znaÄajno povezan sa stadijem tumora. Kaplan-Meierova analiza je pokazala statistiÄki znaÄajnu razliku u ukupnom preživljenju (Cox ph model p=0,016) i preživljenju do progresije (Cox ph model, p=0,0188) bolesnika u odnosu na udio strome. Rezultati su pokazali statistiÄki znaÄajno kraÄe ukupno preživljenje i preživljenje do progresije bolesti kod bolesnika s visokim udjelom strome
IMPACT OF METEOROLOGICAL FACTORS ON SUICIDE ATTEMPTS AND COMPLETED SUICIDES IN CROATIA, OSIJEK-BARANJA COUNTY
Backgraund: Significant changes in the values of temperature, pressure, humidity and cloud cover can participate in the human behaviour including the mood disorders (especially depression and bipolar disorders) which can trigger suicidal ideas or their realization. The aim of this study is to estimate the connection between the aforesaid weather changes and a committed suicide along with the suicide attempts.
Subjects and methods: We conducted a retrospective study in Osijek Baranja County in the period from 2000 to 2011. Meteorological, demographic and epidemiological analysis was performed on 569 suicides and 478 suicide attempts (total of 1047 events). We analyzed the values of the above-mentioned meteorological parameters at the exact time when the event had happened, as well as three days before the event and one day after the event, comparing it with the monthly mean values.
Results: The results showed that the changes of temperature and pressure were significantly related with the suicides i.e. suicide attempts in general, while by separating genders, a connection with all examined meteorological factors was found by woman whereas men expressed significance only in the matter of temperature changes.
Conclusions: Despite the fact that suicidality is caused by numerous factors, this study confirms that a sudden change of weather in the days preceding the suicide can be a trigger to suicidal behaviour. The study also suggests that in the matter of suicidal behaviour, female population is more susceptible to sudden weather changes
PrognostiÄki utjecaj niskog pozitiviteta estrogenih i progesteronskih receptora u luminalnim B (HER2 negativnim) karcinonima dojke
Luminal B (HER2 negative) subtype is the most diversiform type of breast cancers, with a high Ki-67 proliferation index (>20%) or/and low progesterone (PR; <20%) with various intensity and distribution of hormone receptors. Considerable difference has also been noticed in disease outcome, wherefore there is the need for a more detailed classification of this tumor subtype. The clinical and pathologic parameters of 147 luminal B (HER2 negative) breast cancers were examined. The expression of hormone receptors in correlation with other prognostic factors and disease outcome was analyzed by Kaplan-Meier curves and multivariate Cox regression analysis. The Kaplan-Mayer analysis showed that low positivity of estrogen (ER) and PR receptors in tumors was associated with a significantly worse disease outcome (overall survival (ER), p=0.020; disease free survival (ER), p=0.019; overall survival (PR), p=0.026; disease free survival (PR), p=0.038)), unlike Ki-67, which did not show a statistically significant connection (overall survival, p=0.343; disease free survival, p=0.322). The intensity of receptor staining and Ki-67 relative to other histopathologic prognostic factors showed a statistically significant correlation solely with histologic grade of tumor. By using the Cox regression model, PR proved to be an independent prognostic factor for overall survival (p=0.004) and disease free survival (p=0.029). The luminal B (HER2 negative) breast cancer with low expression of hormone receptors, independent of the Ki-67 proliferation index, and in correlation with a higher histologic grade, could be a unique subtype of cancer.Luminalni B (HER 2 negativni) karcinomi dojke najheterogenija su podskupina s velikim rasponom proliferacijskog indeksa Ki-67 kao i intenziteta te raspodjele hormonskih receptora unuatar tumorskih stanica. TakoÄer je zamjetna razliÄitost u kliniÄkom ishodu bolesti, zbog Äega se ukazuje potreba za detaljnijom subtipizacijom ove skupine tumora. Izdvojeno je 147 luminalnih B HER2 negativih karcinoma, a ispitani su kliniÄko-patoloÅ”ki parametri u korelaciji s intenzitetom hormonskih receptora (estrogen (ER) i progesteron (PR)) kao i Ki-67 u odnosu na sveukupno preživljenje te preživljenje bez povrata bolesti. Unutar ispitivanih kliniÄko patoloÅ”kih parametara slabiji intenzitet hormonskih receptora kao i viÅ”i Ki-67 bili su statistiÄki znaÄajno povezani samo s gradusom tumora. Kaplan-Meierovom analizom ustanovljena je statistiÄki znaÄajna povezanost slabog intenziteta ER i PR s preživljenjem (sveukupno preživljenje (ER), p=0,020; preživljenje bez povrata bolesti (ER), p=0,019; sveukupno preživljenje (PR), p=0,026; preživljenje bez povrata bolesti (PR), p=0,038)), za razliku od Ki-67 vrijednosti kojega nisu bile statistiÄki znaÄajno povezane s preživljenjem (sveukupno preživljenje, p=0,343; preživljenje bez povrata bolesti, p=0,322). Coxovom regresijskom analizom PR se pokazao kao statistiÄki znaÄajan neovisni parametar povezan sa sveukupnim preživljenjem (p=0,004) i preživljenjem bez povrata bolesti (p=0,029). Ovi rezultati pokazuju da bi podskupina luminalnih B HER2 negativnih karcinoma dojke s niskim intenzitetom hormonskih receptora, neovisno o proliferacijskom indeksu Ki-67, a u korelaciji s histoloÅ”kim gradusom tumora mogla biti zaseban podtip karcinoma dojke
Angiosarcoma of the Hypopharynx: Case Report
Angiosarcomas are malignant neoplasias of rapid growth that emerge from endothelial cells. They are rarely found in the area of the head and neck and account less than 0.1% of all head and neck malignancies. In some cases it has been linked to trauma, radiation and angiectasia but the etiology mainly remains unknown. Here we report a case of angiosarcoma of pyriform sinus manifested by dysphagia and dysphonia. The patient underwent endoscopic hypopharyngeal excision followed by radiation therapy with a good result. Our patient remains tumor free after two years. The purpose of this article is to add another case of primary angiosarcoma of a rare site, the hypopharynx
Neoadjuvant Chemotherapy Affects TFF3 Peptide Expression in Luminal B Subtype of Breast Cancer ā A Pilot Study
Aim: Trefoil factor family 3 (TFF3) peptide is normally expressed by epithelial cells in breast ducts, but it is also associated with different pathological conditions, including breast cancer. It is considered a marker of poor prognosis and associated with increased resistance to chemotherapy. Data on the effect of chemotherapy on TFF3 peptide expression are scarce. The aim of this pilot study was to assess suitability of research on this topic for large-scale studies.
Methods: Formalin-fixed, paraffin-embedded samples of core biopsies and of surgically removed tumors from patients with luminal B subtype of breast cancer were used for immunohistochemical analysis. Changes in TFF3 peptide and Ki-67 expression and microvessel density (MVD) values before and after chemotherapy were analyzed, as well as the association between TFF3 peptide expression and Ki-67 expression and MVD values.
Results: Significant reduction in TFF3 and Ki 67 expression was observed after chemotherapy, while MVD values did not differ significantly before and after chemotherapy. The association of TFF3 peptide expression and Ki-67 expression and TFF3 peptide expression and MVD values was not significant before or after chemotherapy.
Conclusion: The data obtained in this pilot study suggest that a large-scale study is justified, and it other breast cancer subtypes should be included.
(BijeliÄ N, AbramoviÄ M, Rajc J, RoÄak E, MaruÅ”iÄ Z, ToluÅ”iÄ Levak M, Pauzar B, Belovari T. Neoadjuvant Chemotherapy Affects TFF3 Peptide Expression in Luminal B Subtype of Breast Cancer ā A Pilot Study. SEEMEDJ 2020; 4(2); 20-27
Utjecaj neurotropnih virusa na preživljenje pacijenata operiranih od glioblastoma
Introduction: Glioblastoma represents the most aggressive tumor of the brain
with an estimated survival rate of twelve to fifteen months after the primary diagnosis. The role of
neurotropic viruses in pathogenesis of the tumor has remained unclear to date. During the last two
decades, many studies were conducted with the aim of confirming viral influence in the development
of glioblastoma.
Methods: We conducted a retrospective study in a time period of five years using formalin-fixed
paraffin-embedded tissues of glioblastoma. Immunohistochemistry was performed for three viruses:
CMV, EBV and HSV-1, using an automated staining system.
Results: Mean age of patients in our group was 66.7Ā±8.5 years. A slight male dominance was noted.
Negative immunohistochemistry results were obtained for CMV and EBV, which were excluded
from further investigation. Based on IRS score, we confirmed six HSV-1 samples which were rated as
IRS score 2. Five more samples of HSV-1 were rated as IRS score 1 and were excluded from the study.
Conclusion: According to our retrospective study and its results, we found no impact of neurotropic
viruses in the survival rate of glioblastoma. Further studies should be conducted including a wider
range of viral detection methods.Uvod: Glioblastom predstavlja najagresivniji tumor mozga s procijenjenom stopom preživljenja od dvanaest do petnaest
mjeseci nakon primarne dijagnoze. Uloga neurotropnih virusa u patogenezi tumora do danas je nejasna. Tijekom posljednja
dva desetljeÄa provedena su mnoga istraživanja s ciljem potvrde utjecaja virusa na razvoj glioblastoma.
Metode: Provedena je retrospektivna studija u vremenskom razdoblju od pet godina. KoriŔtena su arhivska tkiva
glioblastoma uklopljena u parafin. Imunohistokemija je raÄena za tri virusa: CMV, EBV i HSV-1 pomoÄu automatiziranog
sustava bojenja.
Rezultati: ProsjeÄna dob bolesnika u naÅ”oj skupini bila je 66,7 Ā± 8,5 godina. Zabilježena je blaža predominacija oboljelih
muÅ”karaca. Dobiveni su negativni imunohistokemijski rezultati za CMV i EBV koji su iskljuÄeni iz daljnjeg ispitivanja.
Prema IRS procjeni potvrdili smo Ŕest uzoraka HSV-1 koji su ocijenjeni kao IRS 2. JoŔ pet uzoraka HSV-1 ocijenjeno je kao
IRS 1 i izuzeti su iz studije.
ZakljuÄak: Prema naÅ”oj retrospektivnoj studiji i posljediÄnim rezultatima, nismo pronaÅ”li utjecaj neurotropnih virusa
na stopu preživljenja u oboljelih od glioblastoma, no potrebna su daljnja istraživanja koja ukljuÄuju Å”iri spektar metoda
dokazivanja virusa
Angiosarcoma of the Hypopharynx: Case Report
Angiosarcomas are malignant neoplasias of rapid growth that emerge from endothelial cells. They are rarely found in the area of the head and neck and account less than 0.1% of all head and neck malignancies. In some cases it has been linked to trauma, radiation and angiectasia but the etiology mainly remains unknown. Here we report a case of angiosarcoma of pyriform sinus manifested by dysphagia and dysphonia. The patient underwent endoscopic hypopharyngeal excision followed by radiation therapy with a good result. Our patient remains tumor free after two years. The purpose of this article is to add another case of primary angiosarcoma of a rare site, the hypopharynx