Detection of Histone Modifications in Plant Leaves

Chromatin structure is important for the regulation of gene expression in eukaryotes. In this process, chromatin remodeling, DNA methylation, and covalent modifications on the amino-terminal tails of histones H3 and H4 play essential roles1-2. H3 and H4 histone modifications include methylation of lysine and arginine, acetylation of lysine, and phosphorylation of serine residues1-2. These modifications are associated either with gene activation, repression, or a primed state of gene that supports more rapid and robust activation of expression after perception of appropriate signals (microbe-associated molecular patterns, light, hormones, etc.)3-7

Mean platelet volume and its prognostic value in acute coronary syndrome complicated by cardiogenic shock

Background: Elevated mean platelet volume (MPV) has been recently discussed as a predictorof death in patients with acute coronary syndrome (ACS), but the cut-off point of MPV inrelation to poor prognosis has not been estimated so far. The aim of this study was to evaluate MPV and its prognostic value in ACS complicated by cardiogenic shock (CS). Such an analysisin patients with the most serious and fatal complication of ACS has not been performed inpreviously published research.Methods: Fifty three patients with ACS complicated by CS (age 68.9 ± 11.4, 49% women, 92% STEMI, 55% fatal CS) and 53 age- and gender-matched patients with uncomplicated ACS as a control group (age 69.1 ± 10.6, 49% women, 92% STEMI, 0% fatal) were includedin our prospective study from 2010 to 2012. All the patients underwent successful primary percutaneous coronary intervention. MPV was determined on admission (MPV1) and in consecutive two days of hospitalization (MPV2, MPV3). The blood sample was analyzed immediately after collection in EDTA tubes using an automatic blood counter.Results: MPV1 was similar in both groups (8.91 ± 1.11 fl vs. 8.57 ± 0.74 fl, NS). Furthermore, there were no statistically significant differences in MPV value in fatal and nonfatal CS(8.90 ± 1.18 fl vs. 8.93 ± 1.05 fl, NS).Conclusions: The above results suggest that MPV cannot be considered a predictor of poorin-hospital outcome in patients with ACS complicated by cardiogenic shock

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mechanismen der Genregulation bei der 'geprimten' Stressantwort in Arabidopsis thaliana

During evolution, plants developed various defense mechanisms against pathogens. One of these mechanisms is called systemic acquired resistance (SAR). SAR confers protection to a broad spectrum of pathogen and also to abiotic stress. SAR is associated with the expression of genes encoding pathogenesis–related proteins and with a process called “defense priming”. “Defense priming” enables the plant to respond to pathogen infection or abiotic stresses in a faster and stronger manner. It is assumed that defense priming is an important part of plant disease resistance and stress tolerance. One type of reaction that gets induced in a faster and more robust manner in primed plants is the induction of defense genes. Gene induction can be regulated in different ways. It is known that, amongst others, transcription factors and histones are important for gene regulation. Histones can be modified thereby controlling the accessibility of regulatory proteins to DNA and chromatin. Amongst the regulatory proteins are transcription factors. These proteins bind to regulatory DNA sequences or to chromatin and subsequent recruit proteins that are necessary for gene induction. In this work histone modifications on the promoters of “primed” genes were investigated. The results show that priming and SAR are associated with an increase of certain histone modifications on defense genes. The investigated genes belong to two different groups. On genes of the one group, – which encode so called WRKY transcription factors – an increase of normally transcription-associated histone modifications was observed. This observation suggests that the WRKY transcription factor genes become prepared (primed) for gene induction. The other group of genes encodes enzymes of the phenylpropanoid pathway and also shows changes in histone modifications. But, according to current knowledge no task can be assigned to these changes. Further experiments determined the role of heat shock transcription factor B1 (HSFB1), a transcription factor that is necessary for defense priming. It could be demonstrated that a mutation of the HSFB1 gene neither affects perception of a pathogen nor the systemic transmission of the SAR inducing signal(s). Although it was not possible to exactly determine the role of HSFB1 in priming, I could show that HSFB1 is a priming specific transcription factor