Validation of the enrichment of candidate miRNAs in exosomes taken up by granulosa cells.

<p>Purified exosomes from follicular fluid of follicles with growing (BCB-) or fully grown (BCB+) oocytes were subjected to total RNA extraction. Then the expression level miR-654-5p and miR-640 (enriched in exosomes derived from BCB- follicular fluid) and miR-526b* and miR-373 (enriched in follicular fluid derived from follicles with BCB+ oocyte) were investigated by real time PCR. Different superscript letters (a,b) denote a significant difference between groups, such that groups not sharing a similar letter are significantly different form each other (P<0.05). The data is presented as means ± s.d. of three biological replicates.</p

Exosome mediated delivery of miRNAs in bovine granulosa cells <i>in vitro</i>.

<p>Purified exosomes from follicular fluid of follicles from growing (Exo BCB-) or fully grown (Exo BCB+) oocytes were co-cultured with bovine granulosa cell. After 24 hrs of incubation at 37°C in a humidified incubator, cells were collected and subjected to total RNA extraction. The expression levels of candidate miRNAs were investigated by real time PCR. In both cases the level of endogenous miRNAs were significantly increase compared to untreated controls. Bars with different superscript letters (a,b) are significantly different (P<0.05) from each other. The data is presented as means ± SD of three biological replicates.</p