Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence

The development of an effective vaccine against human immunodeficiency virus is considered to be the most practicable means of controlling the advancing global AIDS epidemic. Studies with the domestic cat have demonstrated that vaccinal immunity to infection can be induced against feline immunodeficiency virus (FIV); however, protection is largely restricted to laboratory strains of FIV and does not extend to primary strains of the virus. We compared the pathogenicity of two prototypic vaccine challenge strains of FIV derived from molecular clones; the laboratory strain PET<sub>F14</sub> and the primary strain GL8<sub>414</sub>. PET<sub>F14</sub> established a low viral load and had no effect on CD4<sup>+</sup>- or CD8<sup>+</sup>- lymphocyte subsets. In contrast, GL8<sub>414</sub> established a high viral load and induced a significant reduction in the ratio of CD4<sup>+</sup> to CD8<sup>+</sup> lymphocytes by 15 weeks postinfection, suggesting that PET<sub>F14</sub> may be a low-virulence-challenge virus. However, during long-term monitoring of the PET<sub>F14</sub>-infected cats, we observed the emergence of variant viruses in two of three cats. Concomitant with the appearance of the variant viruses, designated 627<sub>W135</sub> and 628<sub>W135</sub>, we observed an expansion of CD8<sup>+</sup>-lymphocyte subpopulations expressing reduced CD8 ß-chain, a phenotype consistent with activation. The variant viruses both carried mutations that reduced the net charge of the V3 loop (K409Q and K409E), giving rise to a reduced ability of the Env proteins to both induce fusion and to establish productive infection in CXCR4-expressing cells. Further, following subsequent challenge of naïve cats with the mutant viruses, the viruses established higher viral loads and induced more marked alterations in CD8<sup>+</sup>-lymphocyte subpopulations than did the parent F14 strain of virus, suggesting that the E409K mutation in the PET<sub>F14</sub> strain contributes to the attenuation of the virus

HI observations of the nearest starburst galaxy NGC 253 with the SKA precursor KAT-7

We present HI observations of the Sculptor Group starburst spiral galaxy NGC 253, obtained with the Karoo Array Telescope (KAT-7). KAT-7 is a pathfinder for the SKA precursor MeerKAT, under construction. The short baselines and low system temperature of the telescope make it very sensitive to large scale, low surface brightness emission. The KAT-7 observations detected 33% more flux than previous VLA observations, mainly in the outer parts and in the halo for a total HI mass of $2.1 \pm 0.1$ $\times 10^{9}$ M$_{\odot}$. HI can be found at large distances perpendicular to the plane out to projected distances of ~9-10 kpc away from the nucleus and ~13-14 kpc at the edge of the disk. A novel technique, based on interactive profile fitting, was used to separate the main disk gas from the anomalous (halo) gas. The rotation curve (RC) derived for the HI disk confirms that it is declining in the outer parts, as seen in previous optical Fabry-Perot measurements. As for the anomalous component, its RC has a very shallow gradient in the inner parts and turns over at the same radius as the disk, kinematically lagging by ~100 km/sec. The kinematics of the observed extra planar gas is compatible with an outflow due to the central starburst and galactic fountains in the outer parts. However, the gas kinematics shows no evidence for inflow. Analysis of the near-IR WISE data, shows clearly that the star formation rate (SFR) is compatible with the starburst nature of NGC 253.Comment: 18 pages, 20 figures, 8 Tables. Accepted for publication to MNRA

Infrared luminosities of galaxies in the Local Volume

Near-infrared properties of 451 galaxies with distances D \leq 10 Mpc are considered basing on the all-sky two micron survey (2MASS). A luminosity function of the galaxies in the K-band is derived within [-25,-11] mag. The local (D < 8 Mpc) luminosity density is estimated to be 6.8*10^8 L_sun/Mpc^3 that exceeds (1.5+-0.1) times the global cosmic density in the K-band. Virial mass-to-K-luminosity ratios are determined for nearby groups and clusters. In the luminosity range of (5*10^{10} - 2*10^{13})L_sun, the groups and clusters follow the relation \lg(M/L_K) propto (0.27+-0.03) lg(L_K) with a scatter of \~0.1 comparable to errors of the observables. The mean ratio ~= (20-25) M_sun/L_sun for the galaxy systems turns out to be significantly lower than the global ratio, (80-90)M_sun/L_sun, expected in the standard cosmological model with the matter density of Omega_m =0.27. This discrepancy can be resolved if most of dark matter in the universe is not associated with galaxies and their systems.Comment: 15 pages, 7 figures. Astronomy Letters, submitte

A search for Low Surface Brightness galaxies in the near-infrared I. Selection of the sample

A sample of about 3,800 Low Surface Brightness (LSB) galaxies was selected using the all-sky near-infrared (J, H and K_s-band) 2MASS survey. The selected objects have a mean central surface brightness within a 5 arcsec radius around their centre fainter than 18 mag/sq.arcsec in the K_s band, making them the lowest surface brightness galaxies detected by 2MASS. A description is given of the relevant properties of the 2MASS survey and the LSB galaxy selection procedure, as well as of basic photometric properties of the selected objects. The latter properties are compared to those of other samples of galaxies, of both LSBs and `classical' high surface brightness (HSB) objects, which were selected in the optical. The 2MASS LSBs have a (B_T_c)-(K_T) colour which is on average 0.9 mag bluer than that of HSBs from the NGC. The 2MASS sample does not appear to contain a significant population of red objects.Comment: accepted for publication in Astronomy and Astrophysics on 24/2/2003; 62 page

Optical Spectroscopic Survey of High-latitude WISE-selected Sources

We report on the results of an optical spectroscopic survey at high Galactic latitude (|b| ≥ 30°) of a sample of WISE-selected targets, grouped by WISE W1 (λ_eff = 3.4 μm) flux, which we use to characterize the sources WISE detected. We observed 762 targets in 10 disjoint fields centered on ultraluminous infrared galaxy candidates using DEIMOS on Keck II. We find 0.30 ± 0.02 galaxies arcmin–2 with a median redshift of z = 0.33 ± 0.01 for the sample with W1 ≥ 120 μJy. The foreground stellar densities in our survey range from 0.23 ± 0.07 arcmin–2 to 1.1 ± 0.1 arcmin–2 for the same sample. We obtained spectra that produced science grade redshifts for ≥90% of our targets for sources with W1 flux ≥120 μJy that also had an i-band flux gsim 18 μJy. We used this for targeting very preliminary data reductions available to the team in 2010 August. Our results therefore present a conservative estimate of what is possible to achieve using WISE's Preliminary Data Release for the study of field galaxies

Gaps in the cloud cover? Comparing extinction measures in spiral disks

Dust in galaxies can be mapped by either the FIR/sub-mm emission, the optical or infrared reddening of starlight, or the extinction of a known background source. We compare two dust extinction measurements for a set of fifteen sections in thirteen nearby galaxies, to determine the scale of the dusty ISM responsible for disk opacity: one using stellar reddening and the other a known background source. In our earlier papers, we presented extinction measurements of 29 galaxies, based on calibrated counts of distant background objects identified though foreground disks in HST/WFPC2 images. For the 13 galaxies that overlap with the Spitzer Infrared Nearby Galaxies Survey (SINGS), we now compare these results with those obtained from an I-L color map. Our goal is to determine whether or not a detected distant galaxy indicates a gap in the dusty ISM, and hence to better understand the nature and geometry of the disk extinction. We find that distant galaxies are predominantly in low-extinction sections marked by the color maps, indicating that their number depends both on the cloud cover of {\it Spitzer}-resolved dust structures --mostly the spiral arms--and a diffuse, unresolved underlying disk. We note that our infrared color map (E[I-L]) underestimates the overall dust presence in these disks severely, because it implicitly assumes the presence of a dust screen in front of the stellar distribution.Comment: 22 pages, 2 figures, 3 tables, accepted for publication in A

Identification of novel subgroup a variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

&lt;b&gt;BACKGROUND:&lt;/b&gt; The development of anaemia in feline leukaemia virus (FeLV)-infected cats is associated with the emergence of a novel viral subgroup, FeLV-C. FeLV-C arises from the subgroup that is transmitted, FeLV-A, through alterations in the amino acid sequence of the receptor binding domain (RBD) of the envelope glycoprotein that result in a shift in the receptor usage and the cell tropism of the virus. The factors that influence the transition from subgroup A to subgroup C remain unclear, one possibility is that a selective pressure in the host drives the acquisition of mutations in the RBD, creating A/C intermediates with enhanced abilities to interact with the FeLV-C receptor, FLVCR. In order to understand further the emergence of FeLV-C in the infected cat, we examined primary isolates of FeLV-C for evidence of FeLV-A variants that bore mutations consistent with a gradual evolution from FeLV-A to FeLV-C.&lt;p&gt;&lt;/p&gt; &lt;b&gt;RESULTS:&lt;/b&gt; Within each isolate of FeLV-C, we identified variants that were ostensibly subgroup A by nucleic acid sequence comparisons, but which bore mutations in the RBD. One such mutation, N91D, was present in multiple isolates and when engineered into a molecular clone of the prototypic FeLV-A (Glasgow-1), enhanced replication was noted in feline cells. Expression of the N91D Env on murine leukaemia virus (MLV) pseudotypes enhanced viral entry mediated by the FeLV-A receptor THTR1 while soluble FeLV-A Env bearing the N91D mutation bound more efficiently to mouse or guinea pig cells bearing the FeLV-A and -C receptors. Long-term in vitro culture of variants bearing the N91D substitution in the presence of anti-FeLV gp70 antibodies did not result in the emergence of FeLV-C variants, suggesting that additional selective pressures in the infected cat may drive the subsequent evolution from subgroup A to subgroup C.&lt;p&gt;&lt;/p&gt; &lt;b&gt;CONCLUSIONS:&lt;/b&gt; Our data support a model in which variants of FeLV-A, bearing subtle differences in the RBD of Env, may be predisposed towards enhanced replication in vivo and subsequent conversion to FeLV-C. The selection pressures in vivo that drive the emergence of FeLV-C in a proportion of infected cats remain to be established