Methodology for the phenotyping resistance to Plum pox virus in peach

Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques

Methodology for detection identification of fungi responsible for the cereal contamination with ochratoxin A and citrinin using PCR:Methodology for practice

The aim of this methodology was to describe a protocol for the rapid and reliable detection of microscopic fungi from the genusPenicillium and Aspergillus capable of producing mycotoxins ochratoxin A and citrinin in cereals, preferably prior to their production and accumulation, using polymerase chain reaction (PCR) and their use, e.g. for the evaluation of health and hygienic safety of plant products

Quantitative trait analysis of resistance to plum pox virus in the apricot F1 progeny "Harlayne" x "Vestar"

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699International audiencePlum pox virus (PPV) is a devastating stone fruit disease of major importance, and better understanding of the genetic control of resistance to this trait would be useful for more efficient development of resistant cultivars. Previous studies have reported a locus of major effect from PPV resistance on linkage group 1. The current study confirms these results by mapping plum pox virus resistance in a F1 progeny issued from a cross between "Harlayne", as a PPV-resistant parent, and "Vestar" as a susceptible parent. The hybrids were grafted simultaneously and subsequently inoculated with the PPV-M and D strains. The symptom scoring on leaves was performed nine times over two vegetative cycles. Marker-trait associations were analyzed using the Kruskal-Wallis (KW) non-parametric test, and the PPV resistance loci were mapped using composite interval mapping (CIM). We show that both analyses (KW and CIM) highlighted the upper part of linkage group 1 of the apricot "Harlayne" genitor