Distinct Macrophage Fates after <i>in vitro</i> Infection with Different Species of <i>Leishmania</i>: Induction of Apoptosis by <i>Leishmania (Leishmania) amazonensis</i>, but Not by <i>Leishmania (Viannia) guyanensis</i>

<div><p><i>Leishmania</i> is an intracellular parasite in vertebrate hosts, including man. During infection, amastigotes replicate inside macrophages and are transmitted to healthy cells, leading to amplification of the infection. Although transfer of amastigotes from infected to healthy cells is a crucial step that may shape the outcome of the infection, it is not fully understood. Here we compare <i>L</i>. <i>amazonensis</i> and <i>L</i>. <i>guyanensis</i> infection in C57BL/6 and BALB/c mice and investigate the fate of macrophages when infected with these species of <i>Leishmania in vitro</i>. As previously shown, infection of mice results in distinct outcomes: <i>L</i>. <i>amazonensis</i> causes a chronic infection in both strains of mice (although milder in C57BL/6), whereas <i>L</i>. <i>guyanensis</i> does not cause them disease. <i>In vitro</i>, infection is persistent in <i>L</i>. <i>amazonensis</i>-infected macrophages whereas <i>L</i>. <i>guyanensis</i> growth is controlled by host cells from both strains of mice. We demonstrate that, <i>in vitro</i>, <i>L</i>. <i>amazonensis</i> induces apoptosis of both C57BL/6 and BALB/c macrophages, characterized by PS exposure, DNA cleavage into nucleosomal size fragments, and consequent hypodiploidy. None of these signs were seen in macrophages infected with <i>L</i>. <i>guyanensis</i>, which seem to die through necrosis, as indicated by increased PI-, but not Annexin V-, positive cells. <i>L</i>. <i>amazonensis</i>-induced macrophage apoptosis was associated to activation of caspases-3, -8 and -9 in both strains of mice. Considering these two species of <i>Leishmania</i> and strains of mice, macrophage apoptosis, induced at the initial moments of infection, correlates with chronic infection, regardless of its severity. We present evidence suggestive that macrophages phagocytize <i>L</i>. <i>amazonensis</i>-infected cells, which has not been verified so far. The ingestion of apoptotic infected macrophages by healthy macrophages could be a way of amastigote spreading, leading to the establishment of infection.</p></div

Loss of nuclear DNA by macrophages after <i>in vitro</i> infection with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i>.

<p>Peritoneal macrophages of BALB/c (A) or C57BL/6 (B) mice infected or not with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i>. After the indicated time points cells were lysed with hypotonic lysis buffer containing PI and nuclei were analyzed by flow cytometry. Bars represent mean ± SD of three independent experiments. A typical experiment is shown in histograms after macrophage nuclei gating and analysis by FlowJo. Gating strategy of cell nuclei is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141196#pone.0141196.s001" target="_blank">S1B and S1C Fig</a>.</p

Caspase-3, -8 and -9 activation in macrophages after <i>in vitro</i> infection with <i>L</i>. <i>amazonensis</i>.

<p>Peritoneal macrophages of BALB/c (left panel) or C57BL/6 mice (right panel) were infected or not (NI) with <i>L</i>. <i>amazonensis</i>. After the indicated time points cell lysates were analysed for caspase activation. Caspase-3 activation was analyzed by Western blot (A) and quantified by densitometry using β-actin and the band above pro-caspase-3 as a load control (B). Caspase-8 and caspase-9 activation was analyzed by CaspaseGlo8 (C) or CaspaseGlo9 (D), both detected by luminescence. For caspases 8 and 9, values represent relative light units (RLU) ± SE from two, three or four (depending on the time point) independent experiments, except for time 14h for caspase-9 in C57BL/6 macrophages a value obtained from one experiment. Statistically significant differences between the two groups indicated, at a <i>P</i> < 0.05, are represented by (*).</p

Exposure of PS by macrophages after <i>in vitro</i> infection with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i>.

<p>Peritoneal macrophages of BALB/c (A) or C57BL/6 (B) mice infected or not with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i> were labeled with AnnV-FITC and counterstained with PI. Analysis was carried out by flow cytometry and only initial moments of apoptosis (AnnV⁺/PI^-) were considered at the indicated time points. Bars represent mean ± SE of two or three (depending on the time point) independent experiments. Statistically significant differences between the two groups indicated, at a <i>P</i> < 0.05, are represented by (*). A typical experiment is shown in dot plots after macrophage gating and analysis by FlowJo (C). Gating strategy is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141196#pone.0141196.s001" target="_blank">S1A Fig</a>.</p

Lesion progression in mice infected with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i> and <i>in vitro</i> infection of macrophages.

<p>Groups of 5 BALB/c (open circles) or C57BL/6 (filled circles) mice were infected in one of the hind footpads with 1x10⁶ stationary phase promastigotes of <i>L</i>. <i>amazonensis</i> (A) or <i>L</i>. <i>guyanensis</i> (B). Lesion progression was assessed weekly for up to 20 weeks by measuring the hind footpads. Lesion size was expressed as the difference between infected and non-infected footpad. Each point represents mean ± SE obtained from 5 mice. Macrophages were infected with <i>L</i>. <i>amazonensis</i> (C, E, G) or <i>L</i>. <i>guyanensis</i> (D, F, H) at a 10:1 parasite:macrophage ratio and incubated for up to 4 days. Infected cells were counted and results are expressed as percentage of infected macrophages (C, D), number of parasite/cell (E, F) and infection index (G, H). Infection index was estimated as the mean percentage of infected cells x the mean number of parasites per infected cells. Bars represent mean ± SE of two to six (depending on the time point) independent experiments. Statistically significant differences between the two groups at a <i>P</i> < 0.05 are represented by (*). Statistically significant differences between values of the same group as compared to values at 24h at a <i>P</i> < 0.05 are represented by (#).</p

<i>In vitro</i> macrophage infection with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i>.

<p>Peritoneal macrophages of BALB/c (left panel) or C57BL/6 mice (right panel) were infected with <i>L</i>. <i>amazonensis</i> or <i>L</i>. <i>guyanensis</i>. After the indicated time points cells were stained with May-Grünwald, followed by Giemsa, method. Images were obtained using QCapture Pro 7 Imaging Software (QImaging) obtained from <a href="http://www.qimaging.com/support/softwarereleases/030107_qcappro.php" target="_blank">http://www.qimaging.com/support/softwarereleases/030107_qcappro.php</a>. Black arrows—amastigotes; green arrows—structures reminiscent of apoptotic bodies or condensed nuclei; yellow arrows—structures reminiscent of disintegrated cells; red arrows—structures reminiscent of apoptotic cells that seem to have been phagocytized by (or are attached in) other macrophages.</p