Monitoring of transcriptional regulation in under protein production conditions-0

<p><b>Copyright information:</b></p><p>Taken from "Monitoring of transcriptional regulation in under protein production conditions"</p><p>http://www.biomedcentral.com/1471-2164/8/179</p><p>BMC Genomics 2007;8():179-179.</p><p>Published online 19 Jun 2007</p><p>PMCID:PMC1919374.</p><p></p>mparison to the wild type. Data from were taken from [21], where UPR was induced with DTT or tunicamycin. ScD60 (treatment with DTT after 60 min); ScD120 (treatment with DTT after 120 min); ScT60 (treatment with tunicamycin after 60 min), all compared to a non-treated culture. Cluster analysis was made using EPClust [], Euklidian distance with complete linkage. Subclusters are shown for the following: A: genes induced in both yeasts; B: upregulated in , down-regulated in ; C: down-regulated to unchanged in , upregulated in ; D: reduced in both yeasts. Subclusters of genes that are unchanged in both organisms are not displayed. The brightest colouring corresponds to the logregulation ≥ ± 2

Monitoring of transcriptional regulation in under protein production conditions-1

<p><b>Copyright information:</b></p><p>Taken from "Monitoring of transcriptional regulation in under protein production conditions"</p><p>http://www.biomedcentral.com/1471-2164/8/179</p><p>BMC Genomics 2007;8():179-179.</p><p>Published online 19 Jun 2007</p><p>PMCID:PMC1919374.</p><p></p>ndend up- or downregulatated genes are highlighted; B: Logratio of cultures producing 2F5 Fab under control of the GAP promoter compared to the wild type, in the same order as A. Red bars: change in transcript up > 2-fold; yellow bars: up > 1.5 fold; white bars: unchanged; blue bars: down > 1.5 fold

Monitoring of transcriptional regulation in under protein production conditions-2

<p><b>Copyright information:</b></p><p>Taken from "Monitoring of transcriptional regulation in under protein production conditions"</p><p>http://www.biomedcentral.com/1471-2164/8/179</p><p>BMC Genomics 2007;8():179-179.</p><p>Published online 19 Jun 2007</p><p>PMCID:PMC1919374.</p><p></p>ts control strain (containing only the Fab expression cassette). B: 2F5 Fab producing SMD1168 co-expressing compared to the control strain. Both diagrams are ordered from the lowest to the highest logratio. Colour legend as in figure 2

Monitoring of transcriptional regulation in under protein production conditions-4

<p><b>Copyright information:</b></p><p>Taken from "Monitoring of transcriptional regulation in under protein production conditions"</p><p>http://www.biomedcentral.com/1471-2164/8/179</p><p>BMC Genomics 2007;8():179-179.</p><p>Published online 19 Jun 2007</p><p>PMCID:PMC1919374.</p><p></p>steady state; Blue bars: 20 °C steady state. BiP: intracellular signals for the UPR marker BiP/Kar2p (detected with anti-Grp78/BiP specific IgG). HC: intracellular signals for Fab heavy chain (obtained with anti-h-Fab specific IgG); LC: intracellular signals for light chain (analyzed with anti-kappa light chain IgG)