1 research outputs found
An <i>in vitro</i> Autogene
Recent technological advances have allowed development
of increasingly complex systems for <i>in vitro</i> evolution.
Here, we describe an <i>in vitro</i> autogene composed of
a self-amplifying T7 RNA polymerase system. Functional autogene templates
in cell-free lysate produce T7 RNA polymerase, which amplifies the
autogene genetic information through a positive feedback architecture.
Compartmentalization of individual templates within a water-in-oil
emulsion links genotype and phenotype, allowing evolution