Plasma aldosterone concentration.

<p>Plasma aldosterone concentration was determined in sham-operated and oophorectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. OVX, oophorectomized. Values are means ± SEM; # <i>P</i><0.05 vs. OVX. n = 7–13/group.</p

Cardiac ACE2 expression.

<p>(A) ACE2 mRNA expression determined by real-time PCR, (B) Western blot for ACE2 in left ventricles and the signal densities quantified using ImageJ, (C) Representative images showing ACE2 staining in the left ventricles, in sham-operated and oophorectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. Values are mean ± SEM; * <i>P</i><0.05 vs. sham, # <i>P</i><0.05 vs. OVX.</p

Cardiac ACE expression and activity.

<p>(A) Cardiac ACE mRNA level determined by real-time PCR, and (B) cardiac ACE activity in sham-operated and oophorectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. Values are mean ± SEM; n = 7–13/group.</p

Plasma and cardiac Ang 1–7 and cardiac mas receptor.

<p>Plasma Ang 1–7, cardiac Ang 1–7 and mas receptor expression were determined in left ventricles of sham-operated and oophorectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. (A) Plasma Ang 1–7 concentration, (B) Representative images showing Ang 1–7 staining in the left ventricles and the intensities quantified using ImageJ, (C) Mas receptor mRNA expression determined by real-time PCR, (D) Western blot for mas receptor in left ventricles. Values are mean ± SEM; * <i>P</i><0.05 vs. sham, # <i>P</i><0.05 vs. OVX.</p

Additional file 1: Table S1. of A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells

Surface protein expression of transduced and non-transduced MSCs originated from dental pulp (DPSC) (n = 3), amniotic fluid (AF-MSCs) (n = 2), and lipoaspirate (ADSC) (n = 2) were analysed by flow cytometry. Data shown represent the mean MFI ± SD obtained in cytometry analysis performed in duplicate. (TIF 177 kb

Cardiac chymase expression and mast cell number.

<p>Chymase expression and mast cell number were determined in the left ventricles of sham-operated and ovariectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. (A) Cardiac chymase mRNA level determined by real-time PCR. (B) Representative images of Western blot for chymase and the signal densities quantified using ImageJ. (C) Correlation analysis of cardiac Ang II staining intensity with cardiac chymase protein level. ○: sham, •: OVX-V, Δ: OVX-E2. (D) Representative images showing mast cell staining. (E) Quantification of cardiac mast cell number. Values are mean ± SEM; # P<0.05 vs. OVX.</p

Serum estradiol concentration.

<p>Serum estradiol concentration in sham-operated and oophorectomized female mRen2.Lewis rats treated with vehicle or estradiol for 4 weeks. OVX, oophorectomized. Values are means ± SEM; * P<0.05 vs. sham, # P<0.05 vs. OVX-V. n = 7–13/group.</p

Additional file 1: of Mesenchymal stromal cell therapy reduces lung inflammation and vascular remodeling and improves hemodynamics in experimental pulmonary arterial hypertension

Determination of pulmonary artery function and Transmission electron microscopy of pulmonary arteries. (DOCX 3690 kb