Enantiomerically pure β-phenylalanine analogues from α–β-phenylalanine mixtures in a single reactive extraction step

An efficient and selective method for the extraction of α-amino acids in preference over their β-isomers using PdCl2(PPh3)2 was discovered, which enables the separation of product mixtures obtained in the enantioselective enzymatic formation of β-amino acids.

Non-Markovian Persistence and Nonequilibrium Critical Dynamics

The persistence exponent \theta for the global order parameter, M(t), of a system quenched from the disordered phase to its critical point describes the probability, p(t) \sim t^{-\theta}, that M(t) does not change sign in the time interval t following the quench. We calculate \theta to O(\epsilon^2) for model A of critical dynamics (and to order \epsilon for model C) and show that at this order M(t) is a non-Markov process. Consequently, \theta is a new exponent. The calculation is performed by expanding around a Markov process, using a simplified version of the perturbation theory recently introduced by Majumdar and Sire [Phys. Rev. Lett. _77_, 1420 (1996); cond-mat/9604151].Comment: 4 pages, Revtex, no figures, requires multicol.st

Cluster Approximation for the Contact Process

The one-dimensional contact process is analyzed by a cluster approximation. In this approach, the hierarchy of rate equations for the densities of finite length empty intervals are truncated under the assumption that adjacent intervals are not correlated. This assumption yields a first order phase transition from an active state to the adsorbing state. Despite the apparent failure of this approximation in describing the critical behavior, our approach provides an accurate description of the steady state properties for a significant range of desorption rates. Moreover, the resulting critical exponents are closer to the simulation values in comparison with site mean-field theory.Comment: 9 pages, Latex format, 2 postscript figure

Precision timing of PSR J1012+5307 and strong-field GR tests

We report on the high precision timing analysis of the pulsar-white dwarf binary PSR J1012+5307. Using 15 years of multi-telescope data from the European Pulsar Timing Array (EPTA) network, a significant measurement of the variation of the orbital period is obtained. Using this ideal strong-field gravity laboratory we derive theory independent limits for both the dipole radiation and the variation of the gravitational constant.Comment: 3 pages, Proceedings of the 12th Marcel Grossmann Meeting on General Relativity (MG 12

First order phase transition with a logarithmic singularity in a model with absorbing states

Recently, Lipowski [cond-mat/0002378] investigated a stochastic lattice model which exhibits a discontinuous transition from an active phase into infinitely many absorbing states. Since the transition is accompanied by an apparent power-law singularity, it was conjectured that the model may combine features of first- and second-order phase transitions. In the present work it is shown that this singularity emerges as an artifact of the definition of the model in terms of products. Instead of a power law, we find a logarithmic singularity at the transition. Moreover, we generalize the model in such a way that the second-order phase transition becomes accessible. As expected, this transition belongs to the universality class of directed percolation.Comment: revtex, 4 pages, 5 eps figure

Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample

BACKGROUND: Inflammatory mediators can serve as biomarkers for the monitoring of the disease progression or prognosis in many conditions. In the present study we introduce an adaptation of a membrane-based technique in which the level of up to 40 cytokines and chemokines can be determined in both human and rodent blood in a semi-quantitative way. The planar assay was modified using the LI-COR (R) detection system (fluorescence based) rather than chemiluminescence and semi-quantitative outcomes were achieved by normalizing the outcomes using the automated exposure settings of the Odyssey readout device. The results were compared to the gold standard assay, namely ELISA. RESULTS: The improved planar assay allowed the detection of a considerably higher number of analytes (n = 30 and n = 5 for fluorescent and chemiluminescent detection, respectively). The improved planar method showed high sensitivity up to 17 pg/ml and a linear correlation of the normalized fluorescence intensity with the results from the ELISA (r = 0.91). CONCLUSIONS: The results show that the membrane-based technique is a semi-quantitative assay that correlates satisfactorily to the gold standard when enhanced by the use of fluorescence and subsequent semi-quantitative analysis. This promising technique can be used to investigate inflammatory profiles in multiple conditions, particularly in studies with constraints in sample sizes and/or budget

Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans

Methylaspartate ammonia lyase (MAL; EC 4.3.1.2) catalyzes the reversible addition of ammonia to mesaconate to give (2S,3S)-3-methylaspartate and (2S,3R)-3-methylaspartate as products. MAL is of considerable biocatalytic interest because of its potential use for the asymmetric synthesis of substituted aspartic acids, which are important building blocks for synthetic enzymes, peptides, chemicals, and pharmaceuticals. Here, we have cloned the gene encoding MAL from the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. The enzyme (named Ch-MAL) was overproduced in Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography. Ch-MAL is a dimer in solution, consisting of two identical subunits (∼49 kDa each), and requires Mg(2+) and K(+) ions for maximum activity. The optimum pH and temperature for the deamination of (2S,3S)-3-methylaspartic acid are 9.0 and 70°C (k(cat) = 78 s(−1) and K(m) = 16 mM). Heat inactivation assays showed that Ch-MAL is stable at 50°C for >4 h, which is the highest thermal stability observed among known MALs. Ch-MAL accepts fumarate, mesaconate, ethylfumarate, and propylfumarate as substrates in the ammonia addition reaction. The enzyme also processes methylamine, ethylamine, hydrazine, hydroxylamine, and methoxylamine as nucleophiles that can replace ammonia in the addition to mesaconate, resulting in the corresponding N-substituted methylaspartic acids with excellent diastereomeric excess (>98% de). This newly identified thermostable MAL appears to be a potentially attractive biocatalyst for the stereoselective synthesis of aspartic acid derivatives on large (industrial) scale. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-011-3615-6) contains supplementary material, which is available to authorized users

Towards Classification of Phase Transitions in Reaction--Diffusion Models

Equilibrium phase transitions are associated with rearrangements of minima of a (Lagrangian) potential. Treatment of non-equilibrium systems requires doubling of degrees of freedom, which may be often interpreted as a transition from the ``coordinate'' to the ``phase'' space representation. As a result, one has to deal with the Hamiltonian formulation of the field theory instead of the Lagrangian one. We suggest a classification scheme of phase transitions in reaction-diffusion models based on the topology of the phase portraits of corresponding Hamiltonians. In models with an absorbing state such a topology is fully determined by intersecting curves of zero ``energy''. We identify four families of topologically distinct classes of phase portraits stable upon RG transformations.Comment: 14 pages, 9 figure

Theory of Branching and Annihilating Random Walks

A systematic theory for the diffusion--limited reaction processes $A + A \to 0$ and $A \to (m+1) A$ is developed. Fluctuations are taken into account via the field--theoretic dynamical renormalization group. For $m$ even the mean field rate equation, which predicts only an active phase, remains qualitatively correct near $d_c = 2$ dimensions; but below $d_c' \approx 4/3$ a nontrivial transition to an inactive phase governed by power law behavior appears. For $m$ odd there is a dynamic phase transition for any $d \leq 2$ which is described by the directed percolation universality class.Comment: 4 pages, revtex, no figures; final version with slight changes, now accepted for publication in Phys. Rev. Let

Branching annihilating random walks with parity conservation on a square lattice

Using Monte Carlo simulations we have studied the transition from an "active" steady state to an absorbing "inactive" state for two versions of the branching annihilating random walks with parity conservation on a square lattice. In the first model the randomly walking particles annihilate when they meet and the branching process creates two additional particles; in the second case we distinguish particles and antiparticles created and annihilated in pairs. Quite distinct critical behavior is found in the two cases, raising the question of what determines universality in this kind of systems.Comment: 4 pages, 4 EPS figures include