33 research outputs found
A Molecular Umbrella Approach to the Intracellular Delivery of siRNA
A series of diwalled and tetrawalled molecular umbrellas have been synthesized using cholic acid, spermidine, and lysine as starting material. Coupling of these molecular umbrellas to an octaarginine peptide afforded agents that were capable of promoting the transport of small interfering RNA (siRNA) to HeLa cells, as judged by the knockdown of enhanced green fluorescent protein (eGFP) expression. The efficiency of this knockdown was found to increase with an increasing number of facially amphiphilic walls present, and also when a cleavable disulfide linker was replaced with a non-cleavable, maleimido moiety. The knockdown efficiency that was observed for one tetrawalled molecular umbrella-octaargine conjugate was comparable to that observed with a commercially available transfection agent, Lipofectamine 2000, but showed less cytotoxicity
Evidence for Highly Cooperative Binding between Molecular UmbrellaâSpermine Conjugates and DNA
A Polymerized Calix[6]arene Monolayer Having Gas Permeation Selectivity that Exceeds Knudsen Diffusion
Minimizing Defects in Polymer-Based LangmuirâBlodgett Monolayers and Bilayers via Gluing
Polymeric surfactants were prepared by quaternization
of polyÂ(4-chloromethylstyrene)
with <i>N</i>,<i>N</i>-dimethyl-<i>N</i>-<i>n</i>-dodecylamine and <i>N</i>,<i>N</i>-dimethyl-<i>N</i>-<i>n</i>-octylamine to give <b>1</b> and <b>2</b>, respectively. Each of these polymers
formed stable monolayers at the air/water interface. Injection of
polyÂ(acrylic acid) (PAA) beneath the surface of these films led to
a substantial increase in their cohesiveness (i.e., âgluingâ),
as evidenced by a dramatic increase in their surface viscosity. Examination
of monolayers of <b>1</b> by atomic force microscopy, after
being transferred to silicon wafers that were surface-modified with <i>n</i>-octadecyltrichlorosilane, showed that the presence of
PAA leads to intact film. In contrast, transfer of unglued monolayers
resulted in poor coverage. Comparison of the barrier properties of
single glued and unglued LB bilayers formed in the presence and in
the absence of PAA have shown that PAA minimizes defect formation
within these ultrathin assemblies