Growth inhibition assay of <i>C. albicans</i> exposed to SBTX (50–400 µg·mL⁻¹).

<p>Fungal growth was measured based on absorbance at 600 nm, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070425#s2" target="_blank">Materials and Methods</a>. The control corresponds to cells grown in the absence of SBTX. Each point represents the mean of three estimates. The standard deviation was less than 10%.</p

Selected <i>C. albicans</i> genes downregulated in response to SBTX exposure.

†<p>Gene names and gene ontology according to the <i>Candida albicans</i> genome database (CGD). ‡ Absolute values<1.50 indicate that genes were downregulated in <i>C. albicans</i> in the presence of SBTX compared with <i>C. albicans</i> cultured without SBTX.</p

Light micrographs of wild type and mutant <i>C. albicans</i> strains in the presence of SBTX.

<p>Cells were incubated in the absence of SBTX (A, C, E) or in the presence of SBTX (B, D, F) (200 µg·mL⁻¹). <i>C. albicans</i> wild type strains (A, B), the <i>C. albicans tup1Δ/tup1Δ</i> mutant (C, D) and the <i>C. albicans rim101Δ/rimΔ mutant</i> (E, F) are shown. Bars (A-F): 10 µm.</p

Transmission electron microscopy (TEM) of <i>C. albicans</i> in the presence of SBTX.

<p>Representative micrographs of single cells observed by TEM of <i>C. albicans</i> cultured in the absence (A) or presence (B, C) of SBTX (400 µg·mL⁻¹). Asterisks indicate condensation and shrinkage of a heavily granulated cytosol and increased vacuolisation in <i>C. albicans</i> treated with SBTX.</p

Selected <i>C. albicans</i> genes upregulated in response to SBTX exposure.

†<p>Gene names and gene ontology according to the <i>Candida albicans</i> genome database (CGD). ‡ Absolute values>1.50 indicate that genes were upregulated in <i>C. albicans</i> in the presence of SBTX compared with <i>C. albicans</i> cultured without SBTX.</p

Functional categories of differentially expressed genes upon treatment with SBTX.

<p>Pie charts showing particular categories of <i>C. albicans</i> genes that were differentially expressed upon treatment with SBTX at 200 µg·mL⁻¹ for 16 h (A) and 18 h (B). A total of 61 genes were clustered for 16 h and 51 genes were clustered for 18 h. The Candida Genome Database (CGD) gene ontology slim mapper was used to cluster these differentially expressed genes into categories (<a href="http://www.candidagenome.org/" target="_blank">http://www.candidagenome.org/</a>).</p