ASSESSMENT OF α- AMYLASE INHIBITORY ACTION OF SOME EDIBLE PLANT SOURCES

Diabetes mellitus is one of the major causes for various manifestations of diseases and clinical complications. Plants are good sources of medicinal compounds and some are traditionally used to control Diabetes mellitus. In this study, the anti-diabetic potential of some edible plants was assessed by α-amylase inhibition assay. Among the studied plants, the extracts of Amaranthus dubius and Alternanthera sessilis were found to possess better inhibition potential against α-amylase enzyme. Acarbose and metformin used as standards for reference showed 67% and 48% inhibition efficiency respectively at a concentration of 250 µg/mL and 500 µg/mL. The results of the study ascertain the use of plants in the control of Diabetes mellitus. The inhibitory action of α-amylase mediated through the synergistic action of the phytoconstituents in the plants, lowers the glycemic level, reducing the risks associated with a sudden increase in blood sugar. The results of the study demonstrate the successful use of in vitro models in screening the plant sources for anti-diabetic activity.Â

Synthesis, antibacterial activity and adsorption studies of Bentonite-iron nanoparticles in the removal of methyl blue dye

Green synthesis of bentonite-iron nanoparticles (Be-FeNPs) has been carried out using the leaf extract of Helicteres isora and its antibacterial and adsorption capacity for the removal of Methyl Blue (MB) dye have been also studied.T he prepared Be-FeNPs and iron nanoparticles (FeNPs) are characterized by UV-visible, FTIR spectroscopic techniques and SEM analysis. The antibacterial activity of Be-FeNPs show good zone of inhibition against E.coli and S.aureus. Further the prepared composite (Be-FeNPs) and bentonite are used as adsorbents for the removal of Methyl Blue (MB) dye in aqueous solution under various experiment parameters like initial concentration, contact time and pH. The optimum initial pH, adsorbent concentration and time were determined to be 8.1, 45°C, and 100 ppm, 80 min for MB adsorption on to Be-FeNPs. The experimental data for MB adsorption fits well to the Freundlich and Langmuir isotherm models. The kinetic equations like Natarajan-Khalaf, Bhattacharya-Venkobachar and Lagergren equations were found to be applicable. The surface morphology of the Be-FeNPs before and after adsorption of dye revealed that it can be used as an excellent adsorbent for dye removal from dilute industrial effluents

ANTIBACTERIAL ACTIVITY OF AQUEOUS ALCOHOLIC EXTRACT OF ABUTILON INDICUM AERIAL PARTS AGAINST E .FAECALIS – AN INVITRO STUDY

Objective: To evaluate the antibacterial activity of the aqueous alcoholic extracts of Abutilon indicum aerial parts against Enterococcus faecalis.Methods: Agar well diffusion assay, as well as microbroth dilution assays, were used for determination of antibacterial activity. The assay was done intriplicate, and chlorhexidine 0.2% was used as the control.Results: Different concentrations (200, 400, and 800 mg/mL) of the ethyl acetate extract of A. indicum aerial parts showed dose-dependantantibacterial activity. The maximum zone of inhibition was 30 mm at the maximum concentration used (800 mg/mL), and the minimum inhibitoryconcentration/minimum bactericidal concentration was found to be 200 mg/mL.Conclusion: This study suggests that the aqueous alcoholic extract of A. indicum aerial parts contains promising antibacterial substances which arehaving activity against E. faecalis. E. faecalis being one of the major threats for root canal failure during endodontic treatment; plants with antibacterialactivity against E. faecalis will be promising

Fabrication of Antimicrobial Perspiration Pads and Cotton Cloth Using Amaranthus dubius Mediated Silver Nanoparticles

Silver nanoparticles prepared through a simplistic method using the aqueous extract of Amaranthus dubius were fabricated on perspiration pads and cotton cloth samples to obtain antibacterial textile materials by two different fabrication methods. The antibacterial activity was investigated against the bacteria Corynebacterium which is commonly present in sweat. Silver nanoparticles that serve as antibacterial agents, against pathogenic bacteria, have gained increased applications in medical devices, textile fabrics, and food industry and hence the result of this study would be a welcomed option

Eco-friendly synthesis of Graphene using the aqueous extract of Amaranthus dubius

An eco-friendly process of reduction of graphene oxide using aqueous extract of Amaranthus dubius under refluxing method is herein reported. The colour change of the graphene oxide (GO) solution from brown to black was noted during the reduction of graphene oxide. UV-Visible spectrophotometer was used to monitor the formation of reduced graphene oxide (AKRGO). The crystallite size of nanographene was confirmed by XRD analysis and Scherrer’s formula. FTIR spectral analysis revealed the reduction of graphene oxide using aqueous extract of Amaranthus dubius. The morphology of the synthesized graphene was examined by SEM analysis

EVALUATION OF ANTHELMINTIC ACTIVITY OF MOMORDICA CHARANTIA, CUCURBITA PEPO L., AND SOLANUM TORVUM BASED FORMULATION AND ITS PHYTOCHEMICAL ANALYSIS USING FOURIER TRANSFORM INFRARED

Objective: In the present study, the phytochemical and anthelmintic effect of a formulation (MCS) prepared from the plants, namely Momordica charantia, Cucurbita pepo L., and Solanum torvum were investigated.Methods: Phytochemical constituents were analyzed using Fourier transform infrared (FTIR), and anthelmintic activity of methanolic and aqueous extract MCS formulation against earthworm Pheretima posthuma was evaluated.Result: As a result of FTIR analysis, MCS formulation showed the presence of coumarin, flavonoids, tannin, phenolic compound, saponin, quinone, and alkaloids. In investigating the anthelmintic action of formulation against adult Indian earthworms, the values of paralytic time and death time of formulation were less when compared to the positive control albendazole.Conclusion: The present work concludes that the MCS formulation acts as a more suitable herbal treatment against helminths infection

Biosynthesis of Silver Nanoparticles Using Kedrostis foetidissima (Jacq.) Cogn.

Nanosilver was synthesized using the aqueous solution of solvent extracts of leaf and stem of Kedrostis foetidissima. Three different methods of formation of silver nanoparticles such as reaction at (i) room temperature, (ii) higher temperature, and (iii) sonication were employed in the present study. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, X-ray diffractometer, Scherrer’s formula, scanning electron microscopy, and FTIR analysis

Biosynthesis of Cubic Gold nanoparticles

Green leafy vegetable (Amaranthus dubius) was used as reductant and capping agent for the synthesis of gold nanoparticles. The nanogold synthesis was carried out under various conditions to evaluate the rate of reduction of gold ions. The formation of gold nanoparticles was monitored by UV-visible spectroscopy. The shape of the nanogold was found to be spherical and the size was analyzed by XRD and SEM analysis. The FTIR measurements confirm that the protein molecule present in the extract aids the formation of gold nanoparticles. Hence green leafy vegetable mediated synthesis of gold nanoparticles may find applications in nutraceuticals

Synthesis, antibacterial activity and adsorption studies of Bentonite-iron nanoparticles in the removal of methyl blue dye

412-420Green synthesis of bentonite-iron nanoparticles (Be-FeNPs) has been carried out using the leaf extract of Helicteres isora and its antibacterial and adsorption capacity for the removal of Methyl Blue (MB) dye have been also studied.T he prepared Be-FeNPs and iron nanoparticles (FeNPs) are characterized by UV-visible, FTIR spectroscopic techniques and SEM analysis. The antibacterial activity of Be-FeNPs show good zone of inhibition against E.coli and S.aureus. Further the prepared composite (Be-FeNPs) and bentonite are used as adsorbents for the removal of Methyl Blue (MB) dye in aqueous solution under various experiment parameters like initial concentration, contact time and pH. The optimum initial pH, adsorbent concentration and time were determined to be 8.1, 45°C, and 100 ppm, 80 min for MB adsorption on to Be-FeNPs. The experimental data for MB adsorption fits well to the Freundlich and Langmuir isotherm models. The kinetic equations like Natarajan-Khalaf, Bhattacharya-Venkobachar and Lagergren equations were found to be applicable. The surface morphology of the Be-FeNPs before and after adsorption of dye revealed that it can be used as an excellent adsorbent for dye removal from dilute industrial effluents