Vicisitudes de un género ensospecha: el cuento argentino del siglo XX

<p>Hum REF is the human reference sequence containing the major alleles. Gene variation among a species is given by an X.</p

<i>FCN1</i>, <i>FCN2</i> and <i>FCN3</i> primers.

<p>Ch: chimpanzee, Go: gorilla, Or: orangutan, Rm: rhesus macaque, Cm: cynomolgus macaque, Ba: baboon, Ma: marmoset.</p><p>*containing a 5′ T7-sequence (5′-taatacgactcactataggg-′3).</p

Alignment of the ficolin-3 protein sequences.

<p>Hum REF is the human reference sequence containing the major alleles. Gene variation among a species is given by an X.</p

Oligomerization pattern of primate ficolin-2 and ficolin-3 was evaluated by SDS-PAGE subjected to western blot.

<p>Samples were analysed by SDS-PAGE on 3–8% Tris-actetate gels under non-reduced and reduced conditions subjected to western blot and detected with (A) mono- and polyclonal anti-ficolin-2 or (B) mono- and polyclonal anti-ficolin-3. Arrows show the multimers and monomers of the ficolins.</p

Illustration of the domain organisation of the human and marmoset <i>FCN1</i> genes (A).

<p>Two alleles of marmosets <i>FCN1</i> were identified. Exons are indicated with black vertical lines. Variations are indicated with blue boxes. Ex: exon, NS AA: non-synonymous amino acid, nt: nucleotide. Alignment of nucleotide/protein sequences of exon 2 of the two <i>FCN1</i> marmoset alleles (B). The marmoset allele 1 sequence has an insertion of 19 nucleotides between nt+1504–1505, which causes a reading frame shift, and subsequently gives rise to a premature stop codon in nt +1514. Alignment of nucleotide/protein sequences of exon 8 and exon 9 of the human and marmoset <i>FCN1</i> genes (C). In exon 8 a nucleotide is deleted in nt +6647, which makes the sequence go out of reading frame. In nt +6687 and +6693 a nucleotide is deleted making the sequence go back into reading frame. These deletions change 15 amino acids in the FBG domain before it goes into reading frame again. In exon 9 eight nucleotides are deleted in nt +7965–7972 and moreover 15 nucleotides are deleted in nt +7986–8000, which causes a reading frame shift and give rise to a premature stop codon, making the ficolin-1 polypeptide ten amino acid shorter in the FBG domain than the human polypeptide.</p

Variation in the <i>FCN2</i> gene.

<p> <b><i>SS:</i></b><i> NCBI submission number, </i><b><i>Nt pos</i></b><i>: nucleotide position, </i><b><i>H-ref</i></b><i>: human reference sequence, </i><b><i>P-ref</i></b><i>: primate reference sequence,</i></p><p> <b><i>Var</i></b><i>: variation detected, </i><b><i>AA change</i></b><i>: amino acid change. The non-synonymous variations are highlighted in bold.</i></p><p>(A) Genetic variations identified in the <i>FCN2</i> gene. (B) Disagreement with the reference sequence in the <i>FCN2</i> gene.</p

Alignment of the <i>FCN2</i> nucleotide sequences.

<p>Hum REF is the human reference sequence containing the major alleles. Gene variation among a species is given by R (A or G), Y (C or T), K(G or T), M (A or C), S (G or C) or W (A or T).</p

Alignment of the <i>FCN3</i> nucleotide sequences.

<p>Hum REF is the human reference sequence containing the major alleles. Gene variation among a species is given by R (A or G), Y (C or T), K(G or T), M (A or C), S (G or C) or W (A or T).</p

Variation in the <i>FCN3</i> gene.

<p> <b><i>SS:</i></b><i> NCBI submission number, </i><b><i>Nt pos</i></b><i>: nucleotide position, </i><b><i>H-ref</i></b><i>: human reference sequence, </i><b><i>P-ref</i></b><i>: primate reference sequence,</i></p><p> <b><i>Var</i></b><i>: variation detected, </i><b><i>AA change</i></b><i>: amino acid change. The non-synonymous variations are highlighted in bold.</i></p><p>(A) Genetic variations identified in the <i>FCN3</i> gene. (B) Disagreement with the reference sequence in the <i>FCN3</i> gene.</p

Phylogenetic relationship of the primate ficolins based on the proteins sequences.

<p>The neighbouring joining was bootstrapped 500 times. Based on several output files representative phylogenetic trees were constructed. Numbers on branches are bootstrap percentages supporting a given partitioning.</p