Digital Manufacturing in SMEs based on the context of the Industry 4.0 framework-one approach

Serbia is rapidly working on the development and implementation of digital manufacturing models in SMEs, through the national Industry 4.0 Platform. The aim is to create a pilot intelligent workshop which would be used to develop and showcase examples of best practice for digital manufacturing. Currently, most SMEs use CAD, CAM, ERP models, which form the basis for the development of the concept of digital manufacturing through cloud computing, BDA, IIoT and smart supply-chains, as elements of Industry 4.0. This paper gives a practical example of an SME with all the above-mentioned elements of digital manufacturing

Digital Manufacturing in SMEs based on the context of the Industry 4.0 framework-one approach

Serbia is rapidly working on the development and implementation of digital manufacturing models in SMEs, through the national Industry 4.0 Platform. The aim is to create a pilot intelligent workshop which would be used to develop and showcase examples of best practice for digital manufacturing. Currently, most SMEs use CAD, CAM, ERP models, which form the basis for the development of the concept of digital manufacturing through cloud computing, BDA, IIoT and smart supply-chains, as elements of Industry 4.0. This paper gives a practical example of an SME with all the above-mentioned elements of digital manufacturing

MCL-1 promiscuity and the structural resilience of its binding partners

The allosteric protein MCL-1 and its natural inhibitors, the BH3-only proteins PUMA, BIM, and NOXA regulate apoptosis by interacting promiscuously within an entangled binding network. Little is known about the transient processes and dynamic conformational fluctuations that are the basis for the formation and stability of the MCL-1/BH3-only complex. In this study, we designed photoswitchable versions of MCL-1/PUMA and MCL-1/NOXA, and investigated the protein response after an ultrafast photo-perturbation with transient infrared spectroscopy. We observed partial α-helical unfolding in all cases, albeit on strongly varying timescales (1.6 ns for PUMA, 9.7 ns for the previously studied BIM, and 85 ns for NOXA). These differences are interpreted as a BH3-only-specific “structural resilience” to defy the perturbation while remaining in MCL-1’s binding pocket. Thus, the presented insights could help to better understand the differences between PUMA, BIM, and NOXA, the promiscuity of MCL-1, in general, and the role of the proteins in the apoptotic network

MCL-1 promiscuity and the structural resilience of its binding partners

MCL-1 and its natural inhibitors, the BH3-only proteins PUMA, BIM, and NOXA regulate apoptosis by interacting promiscuously within an entangled binding network. Little is known about the transient processes and dynamic conformational fluctuations that are the basis for the formation and stability of the MCL-1/BH3-only complex. In this study, we designed photoswitchable versions of MCL-1/PUMA and MCL-1/NOXA, and investigated the protein response after an ultrafast photo-perturbation with transient infrared spectroscopy. We observed partial $\alpha$-helical unfolding in all cases, albeit on strongly varying timescales (1.6~ns for PUMA, 9.7~ns for the previously studied BIM, and 85~ns for NOXA). These differences are interpreted as a BH3-only-specific "structural resilience" to defy the perturbation while remaining in MCL-1's binding pocket. Thus, the presented insights could help to better understand the differences between PUMA, BIM, and NOXA, the promiscuity of MCL-1 in general, and the role of the proteins in the apoptotic network

Factors affecting labelling yield of 111In-DTPA-BSA

Radiolabelling of antibodies depends on a number of factors including the chemical characteristics of the nuclide and the techniques employed for its incorporation into protein. For preliminary research we used model system and investigate the influence of different factors affecting labelling. Obtained results were successfully used for further radiolabelling of antibodies with different trivalent metals.Physical chemistry 2006 : 8th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-29 September 200

Assessment of synergistic effect on performing the co-pyrolysis process of coal and waste blends based on thermal analysis

The preliminary thermogravimetric studies of co-pyrolyzed low rank coals (lignites Kostolac and Kolubara) with waste materials (spent coffee ground and waste rubber granulate) in a form of blends have been performed. Thermal analysis measurements of blend samples were carried out in a nitrogen, atmosphere at three different heating rates of 10, 15, and 20 K per minute. The coal-waste blends were prepared in the percentage ratios of 90:10, 80:20, and 70:30. This work analyzed the synergy analysis for considered blends shown via descriptive parameters during co-pyrolysis process. According to the performed analysis, the presence of synergistic effect was identified, where strong interactions were also observed. For lignite-spent coffee ground blends, it was found that two factors which affect the synergy effect with coal are concentration of added biomass material and the heating rate. For lignite-tire rubber granulate blends, the blending ratio take on a decisive role for positive consequences of a synergistic effect (ratios below 30% of tire rubber granulate in coals are desirable). Also, in this work the influence of micro-scale condition parameters such as heating rate (as the experimental regulatory factor) was analyzed on the magnitude response of synergism during co-pyrolysis

Predictors of burnout among Belgrade veterinary students: A cross-sectional study

Background To the best of our knowledge, studies are lacking on burnout among veterinary students in Serbia, and this is the first study trying to address such a problem. Therefore, the aim of this cross-sectional study was to investigate the predictors of burnout among Belgrade veterinary students. Methods Maslach Burnout Inventory (MBI) and anonymous structured questionnaire addressed to personal data, health habits and stressful influence of educational process were applied among 496 respondents from a total of 1113 students from all grades in spring semester 2014 (response rate 44.6%). Results The prevalence of burnout was 43.3%. High scores on depersonalization and emotional exhaustion scales of MBI were found among 79.4% and 45.0% students, respectively; low personal accomplishment was reported by 50.5% students. Female students reported higher levels of emotional exhaustion compared to males (p = 0.012). A low score on personal achievement scale of MBI was least frequent among the freshmen and most frequent among sophomores (41.1% and 65.3%, respectively; p = 0.986). There were more students with burnout who considered their health as a worsened vs. pre-study period compared to students with no burnout, both concerning mental (61.4% vs. 38.6%) and physical health (61.1% vs. 38.9%; both p lt 0.001). There were more smokers among students who suffered from burnout compared to students who did not (52.0% vs. 48.0%; p = 0.013). A multiple regression revealed an independent dose-response effect of perceived stress at exams on the onset of burnout (moderate stress OR = 2.164 and high stress OR = 3.878). Also, students with the moderate and high stressful effect of communication with teaching staff, as well as, those with worse self-perceived physical and mental health had more than two times higher presence of burnout. Conclusions The prevalence of burnout among Belgrade veterinary students is relatively high. Primary prevention should be focused on the revealed predictors of burnout

Uticaj azota na prinos zelene biomase i suve materije Sorghum sp. vrsta po fazama rastenja

The paper investigated the production properties of three sorghum genotypes: NS-Džin (forage sorghum), Zora (Sudan grass) and Siloking (interspecies hybrid) in terms of different nitrogen rates used in side dressing in 2009 and 2010. The subject of study was green biomass and dry matter yield in the stages of intensive growth and tasseling. The results have shown that there have been significant fluctuations in production indicators between the genotypes. In the total average, the lowest yield was recorded for the Sudan grass (85.41 t ha-1). Significantly higher yields were recorded for the interspecies hybrid (90.22 t ha-1) and the forage sorghum (93.51 t ha-1). Although the effect of nitrogen rates depended on weather conditions, i.e. rainfall distribution, the optimal nitrogen rate in both years was 180 kg ha-1.U radu su proučavane produktivne osobine tri genotipa sirka i to: NS-Džin (krmni sirak), Zora (sudanska trava) i Siloking (interspecijes hibrid) u zavisnosti od upotrebljenih količina azota za dopunsku ishranu biljaka tokom 2009. i 2010. godine. Ispitivani su prinos zelene mase u fazama intezivnog porasta i metličenja. Između ispitivanih genotipova postoje značajna variranja u pokazateljima produktivnosti. U ukupnom proseku najmanji prinos dala je sudanska trava 85,41 t ha-1. Značajno viši prinos bio je kod interspecijes hibrida (90,22 t ha-1) i krmnog sirka (93,51 t ha-1). Iako je efekat upotrebljenog azota zavisio od vremenskih uslova, odnosno od rasporeda padavina, u obe godine optimalna količina azota bila je 180 kg ha-1