Environmental risk assessment studies on new plant protection products which have been elaborated from coniferous tree bark

ArticleNowadays there are still various chemical pesticides being applied in the course of ensuring plant protection. Since 2010, we have been working on the development of new, environmentally-friendly plant protection products which will provide an effective tool against pathogenic fungi and bacteria which cause disease in crop plants. The specific aim of this study was to evaluate a risk assessment for new plant protection products that have been elaborated on the basis of coniferous tree bark. Various products were tested which are extracted during the processing of wood bark from pine (Pinus sylvestris L.) and spruce (Picea abies (L.) Karst.). Ethanol extracts were formulated and applied during these experiments. Two formulations, which showed anti-fungal activity in vitro and in field trials on fruit crops (involving strawberries and raspberries) were selected for the risk assessment studies. The impact was studied of formulation treatment on crop plants and soil biological activity, and the accumulation of residues of active substances in crop plants and soil. The application of new formulations did not show any negative effect on the chlorophyll content and the chlorophyll fluorescence of plant leaves. The results showed that pine and spruce bark extract formulations contain active compounds (coumaric acid, quercetin, epicatechin, and ferulic acid) within the range of 5.1–5.9 mg kg-1 and 11.1– 443.9 mg kg-1 respectively. The amount of active substances which were determined in most cases was higher in the spruce bark extract formulation when compared to the pine bark extract formulation. Our results confirmed the presence of active compounds – epicatechin, quercetin, and coumaric acid – in strawberry fruits which remained untreated and in those that were treated with spruce ethanol extract formulation. Untreated raspberry fruits contained all four active substances within the range of 81–5,300 µg kg-1. We observed a significant increase of coumaric acid and quercetin in raspberries after their having been treated with spruce bark extract formulation in a 2% concentration, P < 0.05, and did not find any negative impact for spruce bark extract formulations when used on soil microbial biomass

The microbiome of pest insects:It is not just bacteria

Insects are associated with multiple microbes that have been reported to influence various aspects of their biology. Most studies in insects, including pest species, focus on the bacterial communities of the microbiome even though the microbiome consists of members of many more kingdoms, which can also have large influence on the life history of insects. In this review, we present some key examples of how the different members of the microbiome, such as bacteria, fungi, viruses, archaea, and protozoa, affect the fitness and behavior of pest insects. Moreover, we argue that interactions within and among microbial groups are abundant and of great importance, necessitating the use of a community approach to study microbial-host interactions. We propose that the restricted focus on bacteria very likely hampers our understanding of the functioning and impact of the microbiome on the biology of pest insects. We close our review by highlighting a few open questions that can provide an in-depth understanding of how other components of the microbiome, in addition to bacteria, might influence host performance, thus contributing to pest insect ecology

The Importance and Potential Value of a Regional Midsize Arthropod Collection: An Example of IBULC

During the last three centuries, people involved in natural history sciences have produced thousands of natural history collections worldwide. Specimens have been used for scientific proof of various discoveries and for gaining new knowledge in many disciplines of science not only in the area of biodiversity but also in taxonomy, species biology and ecology, parasitology, evolution, organisms’ responses to climate change, nature conservation and many other biological sub-disciplines. Despite never-ending financial struggles, the world’s largest collections as well as many regional midsize collections contain billions of specimens. This provides an enormous database for present and future studies. This article presents a critical description of the material of the Institute of Biology, University of Latvia collection (IBULC) containing roughly 59,000 identified arthropod individuals of about 3,700 species, including twenty-eight primary types as a part of around ninety type specimens, to illustrate the importance of midsize regional natural history collections in detail.Some Rights Reserved. Public Licensed Material: Available under the terms and conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Public License (CC BY-NC-ND 4.0). The use of this material is permitted for non-commercial use provided the creator(s) and publisher receive attribution. No derivatives of this version are permitted. Official terms of this public license apply as indicated here: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcod

Effects of fertilization on Picea abies stands situated on drained peat soils

ArticleNorway spruce used for afforestation of drained peat soils frequently has low productivity and decay in a long - term, which could be related to soil chemical composition and nutrient status. The research aim was to elucidate the effect of PSM on new Norway spruce plantings (1st experiment) and 20 - year - old spruce stands (2nd experiment) on drained peat soils by evaluating: (1) nutrient accumulation in soil - plant system, (2) soil microbiological activity, (3) health status of spruce individuals, (4) growth int ensity and productivity of spruce, (5) changes in composition of vascular plant and moss species. The study was conducted at two forest ( Myrtillosa turf.mel .) sites in Latvia, each consisting of two plots: control and treated with PMS (100 g m - 2 in Septemb er 2007, 50 g m - 2 in April 2008). During 2008 – 2016, regular analysis of soil, spruce needles, soil microbiology, assessment of tree crown vitality, stand productivity, inventory of vascular plant and moss species were done. The results showed that the fert ilization with PMS resulted in a significantly improved K, Ca, Zn, and N status of trees, crown vitality and up to three times increased tree growth parameters at both experiments during the study period. The average count of bacteria and fungi in soil of fertilized plots, accompanied by a remarkable variability in the study years, was significantly higher only for the 1 st experiment. Fungi : bacteria ratio for the fertilized and control plots differed significantly only for the 1 st experiment. Significant i ncrease of cover with nitrophilic plant ( Urtica dioica, Antriscus sylvestris, Rubus idaeus ) and moss ( Plagiomnium cuspidatum , P. ellipticum ) species at both fertilized sites were stated