(A) SEM cartography of sample Pi4 Mg2 obtained with a magnification of 400 at 1 KeV (B) spatial distribution of Pi (purple maps), spatial distribution of Ca2+ (red maps) as obtained by EDX and SEM cartography (right pictures) obtained at 12 KeV for samples Pi4, Pi4 Mg2 and Pi4 Mg5.

<p>(A) SEM cartography of sample Pi4 Mg2 obtained with a magnification of 400 at 1 KeV (B) spatial distribution of Pi (purple maps), spatial distribution of Ca2+ (red maps) as obtained by EDX and SEM cartography (right pictures) obtained at 12 KeV for samples Pi4, Pi4 Mg2 and Pi4 Mg5.</p

Typical optical image and mapping (scale from blue to red with increasing concentration), and FTIR spectra of crystals generated in Pi 4 or PiCa samples without or in presence of a total concentration of 2 mM of Mg²⁺.

<p>Ia) Optical image, and corresponding Ib) typical FTIR spectra, Ic) Optical image and corresponding Id) typical FTIR spectra of sample PiCa. IIa) Optical image and corresponding IIb) typical FTIR spectra, IIc) Optical image and corresponding IId) typical FTIR spectra of sample PiCa Mg2.</p

µFTIR maps obtained with calcium phosphate apatite features were collected for samples: PiCa, PiCa Mg2, PiCa Mg5, Pi4, Pi4 Mg2, Pi4 Mg5.

<p>The scale bar corresponds to 500 µm while the amplitude is between 0 and 10. Due to extended acquisition times, some maps were divided in 2 areas. The map size is 2000 µm x 4500 µm.</p

Identification of the samples studied through µFTIR experiments.

<p>Samples nomenclature</p><p><b>Ct</b> = samples incubated in 1% FBS DMEM</p><p><b>Pi3</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM</p><p><b>Pi4</b> = samples incubated in 1% FBS DMEM with Pi set to 4 mM</p><p><b>Pi3 Mg2</b> = incubated in 1% FBS DMEM with Pi set to 3 mM and Mg²⁺ set to 2 mM</p><p><b>Pi4 Mg1.5</b> = incubated in 1% FBS DMEM with Pi set to 4 mM and Mg²⁺ set to 1.5 mM</p><p><b>Pi4 Mg2</b> = incubated in 1% FBS DMEM with Pi set to 4 mM and Mg²⁺ set to 2 mM</p><p><b>Pi4 Mg5</b> = incubated in 1% FBS DMEM with Pi set to 4 mM and Mg²⁺ set to 5 mM</p><p><b>PiCa</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM and Ca²⁺ set to 2.4 mM</p><p><b>PiCa Mg1.5</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM, Ca²⁺ set to 2.4 mM and Mg²⁺ set to 1.5 mM</p><p><b>PiCa Mg2</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM, Ca²⁺ set to 2.4 mM and Mg²⁺ set to 2 mM</p><p><b>PiCa Mg5</b> = samples incubated in 1% FBS DMEM with Pi set to 3 mM, Ca²⁺ set to 2.4 mM and Mg²⁺ set to 5 mM</p><p>It is of note that these experiments were conducted concomitantly with and without cells. The table is indicating the presence of apatite in experiments with cells. Data of passive Ca / Pi deposition on MirrIR slides are discussed in a proper section.</p><p>Identification of the samples studied through µFTIR experiments.</p

Urinary phosphorus levels in 5/6^th nephrectomized rats on a normal (NP) or high phosphorus (HP) diet treated with either vehicle (veh), 185 or 375 mg/kg/day CaMg.

<p>*: p<0.05 vs NP, same dose; #: p<0.05 vs vehicle, same diet; °: p<0.05 vs week −4, same diet and dose.</p

Calcium and magnesium content in the tibia of 5/6^th nephrectomized rats on a normal (NP) or high phosphorus (HP) diet treated with either vehicle (veh), 185 or 375 mg/kg/day CaMg.

<p>Calcium and magnesium content in the tibia of 5/6^th nephrectomized rats on a normal (NP) or high phosphorus (HP) diet treated with either vehicle (veh), 185 or 375 mg/kg/day CaMg.</p

Static and dynamic bone parameters of 5/6^th nephrectomized rats on a normal (NP) or high phosphorus (HP) diet treated with either vehicle (veh), 185 or 375 mg/kg/day CaMg.

<p>*: p<0.05 vs. NP, same dose.</p

Urine biochemistry over time of 5/6^th nephrectomized rats receiving normal phosphorus (NP) or high phosphorus (HP) diet, in combination with vehicle (Veh), 185 or 375 mg/kg CaMg.

<p>*: p<0.05 vs NP, same dose.</p><p>°: p<0.05 vs week −4, same diet and dose.</p><p>Urine biochemistry over time of 5/6^th nephrectomized rats receiving normal phosphorus (NP) or high phosphorus (HP) diet, in combination with vehicle (Veh), 185 or 375 mg/kg CaMg.</p

Aortic calcification in vehicle versus 185 mg/kg CaMg treated chronic renal failure (CRF) rats.

<p>*: p<0.05 vs. CRF + vehicle.</p

Serum biochemistry of vehicle versus 185 mg/kg CaMg treated adenine-induced CRF rats.

<p>*: p<0.05 vs. vehicle treated CRF rats, same time point.</p><p>°: p<0.05 vs. week 0, same treatment group.</p><p>Serum biochemistry of vehicle versus 185 mg/kg CaMg treated adenine-induced CRF rats.</p