Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood-1

Atelets and bacteria. (B) Neutrophils and monocytes were identified by setting a polygonal gate in a forward scatter/sideward scatter dot plot. The negative control sample (C) was used to define a marker for rhodamine 123 (FL 1) where less than 5% of the cells would be positive. The percentage of neutrophils having produced hydrogen peroxide following lipid incubation was determined by counting the number of rhodamine positive cells above this marker position and by dividing it by the whole number of events observed (D).<p><b>Copyright information:</b></p><p>Taken from "Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood"</p><p>http://www.nutritionandmetabolism.com/content/5/1/19</p><p>Nutrition & Metabolism 2008;5():19-19.</p><p>Published online 14 Jul 2008</p><p>PMCID:PMC2483276.</p><p></p

Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood-0

Atelets and bacteria. (B) Neutrophils and monocytes were identified by setting a polygonal gate in a forward scatter/sideward scatter dot plot. The negative control sample (C) was used to define a marker for rhodamine 123 (FL 1) where less than 5% of the cells would be positive. The percentage of neutrophils having produced hydrogen peroxide following lipid incubation was determined by counting the number of rhodamine positive cells above this marker position and by dividing it by the whole number of events observed (D).<p><b>Copyright information:</b></p><p>Taken from "Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood"</p><p>http://www.nutritionandmetabolism.com/content/5/1/19</p><p>Nutrition & Metabolism 2008;5():19-19.</p><p>Published online 14 Jul 2008</p><p>PMCID:PMC2483276.</p><p></p