Regulation of 11beta-HSD1 and differentiation markers during differentiation of C2C12 cells and primary human myoblasts.

<p>Data were normalized to mRNA expression of 16s-RiboProtein. Mean ± SEM of at least three experiments are shown. Relative 11beta-HSD1 mRNA expression in C2C12 (<b>A</b>). Relative Myosin Heavy Chain-1 mRNA expression in C2C12 cells (<b>B</b>). Relative Myf5 mRNA expression in C2C12 cells (<b>C</b>). Relative 11beta-HSD1 mRNA expression in primary human myoblasts (<b>D</b>). Relative Myosin Heavy Chain-1 mRNA expression in primary human myoblasts (<b>E</b>). * p<0.05, ** p<0.01, *** p<0.0001.</p

Effects of cortisone, dexamethasone (dexa) and inhibition of 11beta-HSD1 by carbenoxolone (Cbx) on Atrogin-1 and MuRF-1 expression.

<p>Relative Atrogin-1 and MuRF-1 mRNA expression in C2C12 (<b>A and B</b>) and in primary human myotubes (<b>C and D</b>). Data are normalized to mRNA expression of 16s-RiboProtein. Mean ± SEM of at least three experiments are shown. * p<0.05, ** p<0.01, *** p<0.0001.</p

Effects of cortisone, dexamethasone (dexa) and inhibition of 11beta-HSD1 by carbenoxolone (Cbx) on protein degradation in C2C12 myotubes.

<p>Protein degradation was measured by determining the rate of release of TCA-soluble proteins radioactively labeled with [³H]-tyrosine into the media. Mean ± SEM of at least three experiments are shown. * p<0.05, ** p<0.01, *** p<0.0001.</p