Eumycetoma on the foot caused by Madurella mycetomatis: amputation after significant worsening during pregnancy

Submitted by Janaína Nascimento ([email protected]) on 2019-03-26T12:48:51Z No. of bitstreams: 1 ve_Sampaio_Felipe_etal_INI_2015.pdf: 859446 bytes, checksum: 1f4612e074c315c29dadaa28e4b78ec3 (MD5)Approved for entry into archive by Janaína Nascimento ([email protected]) on 2019-03-27T14:43:47Z (GMT) No. of bitstreams: 1 ve_Sampaio_Felipe_etal_INI_2015.pdf: 859446 bytes, checksum: 1f4612e074c315c29dadaa28e4b78ec3 (MD5)Made available in DSpace on 2019-03-27T14:43:47Z (GMT). No. of bitstreams: 1 ve_Sampaio_Felipe_etal_INI_2015.pdf: 859446 bytes, checksum: 1f4612e074c315c29dadaa28e4b78ec3 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Rio de Janeiro State University. Orthopedic Ambulatory. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Laboratório de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil

Eumycetoma by Madurella mycetomatis with 30 years of evolution: therapeutic challenge

We report a case of eumycetoma by Madurella mycetomatis on the buttocks and thighs in an adult immunocompetent patient, diagnosed after 30 years of clinical development. He was treated over four years with fluconazol and itraconazol associated with five times surgical excisions of subcutaneous nodules. At the eighth year of follow-up, one nodule recurred on the right infragluteal region, which was excised surgically and has remained asymptomatic ever since

Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues

Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations.Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infected with tachyzoites (RH strain), the acute infection group (AIG). Tissues were subjected to DNA extraction followed by PCR amplification of T. gondii B1 gene. PCR recovered T. gondii DNA in thigh muscle, encephalon, heart, and lung samples. AIG presented PCR positivity in encephalon, lungs, hearts, and livers. Based on this results, we propose this molecular approach for toxoplasmosis research in past populations