Effect of soil and water environment on typeability of PowerPlex Y (Promega) in selected tissue samples.

In cases of decomposed bodies Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability PowerPlex Y (Promega) loci in tissue material stored in water and soil environment. Tissue material was collected during autopsies of five persons aged 20-30 years with time of death determined within the limit of 14 hours. Heart muscle, liver and lung specimens were stored in pond water, sea water, sand and peat soil. DNA was extracted by organic method from tissue samples collected in 7-day intervals. Liver specimens were typeable in all PowerPlex Y loci within 100 days of storage in pond water with gradual decline at DYS392 in sea water. Heart muscle specimens stored in pond water exhibited allelic loss at DYS19, DYS385, DYS389II and DYS392, while all loci were typeable in sea water stored samples. For lung specimens allelic loss was noted throughout the profile. Storage of liver specimens in peat soil for more than 14 days resulted in allelic drop-out, and after 21 days no profiles were typeable. Heart muscle specimens were typeable in all PowerPlex Y systems after 35-day storage in sand, while allelic drop-out and subsequent lack of profiles were noted after 14 and 35 days respectively. Lung specimens stored in garden soil exhibited allelic drop-out and subsequent lack of profiles after 7 and 21 days, respectively. All PowerPlex Y loci were typeable in the latter material in sand up to day 35 with gradual decline of longer amplicons (DYS19, DYS385, DYS389II and DYS392)

Leczenie mózgowych malformacji tętniczo-żylnych metodą przezskórnej embolizacji

Background: In this paper we present our results of treating cerebral arteriovenous malformations implementing percutaneous embolization between 2001-2003. Material/Methods: From year 2001 to 2003, a group of 34 patients underwent endovascular embolization of cerebral arteriovenous malformations. This group consisted of 10 female and 24 male patients aged 15 to 78 (mean: 45.3). DSA examination was performed to assess the possibility of percutaneous embolization. Microcatheters 1.2 F, 1.5 F, or 1.8 F were used in accordance with the dimensions of the malformation. The tip of the catheter was placed close to the nidus of the malformation. Results: Cerebral arteriovenous malformations were localized mainly in the temple region (38%) or in the parietal region (27%). Primary embolization resulted in total occlusion in 41% of AVMs fed by up to three vessels, Repeated interventions led to occlusion of the additional hemangiomas in up to 70.5% of all lesions. In cases of AMVs fed by more than 4 vessels, the second embolization did not result in total occlusion of the hemangiomas. Curative results were obtained in only 12 patients (35%). Total or partial occlusion resulted in the partial reduction of the diameters of the malformations by 30 to 80% (mean: 74%). Conclusions: The best results of embolization were achieved in cases of small and medium malformations fed by 1-3 vessels (70.5% of cases). In our opinion, percutaneous embolization could be applied as an independent curative method in adult patients with small and medium arteriovenous malformations, grades I - III of the Spetzler-Martine scale