1 research outputs found
Adaptation in Caco‑2 Human Intestinal Cell Differentiation and Phenolic Transport with Chronic Exposure to Blackberry (<i>Rubus</i> sp.) Extract
As
evidence mounts for a health-protective role of dietary phenolics,
the importance of understanding factors influencing bioavailability
increases. Recent evidence has suggested chronic exposure to phenolics
may impact their absorption and metabolism. To explore alterations
occurring from chronic dietary exposure to phenolics, Caco-2 cell
monolayers were differentiated on Transwell inserts with 0–10
μM blackberry (<i>Rubus</i> sp.) total phenolics extracts
rich in anthocyanins, flavonols, and phenolic acids. Following differentiation,
apical to basolateral transport of phenolics was assessed from an
acute treatment of 100 μM blackberry phenolics from 0 to 4 h.
Additionally, differences in gene expression of transport and phase
II metabolizing systems including ABC transporters, organic anion
transporters (OATs), and uridine 5'-diphospho (UDP) glucuronosyltransferases
(UGTs) were probed. After 4 h, 1 μM pretreated monolayers showed
a significant (<i>P</i> < 0.05) decrease in the percentage
of cumulative transport including less epicatechin (42.1 ± 0.53),
kaempferol glucoside (23.5 ± 0.29), and dicaffeoylquinic acid
(31.9 ± 0.20) compared to control. Finally, significant (<i>P</i> < 0.05) alterations in mRNA expression of key phase
II metabolizing enzymes and transport proteins were observed with
treatment. Therefore, adaptation to blackberry extract exposure may
impact intestinal transport and metabolism of phenolics