22.二酸化窒素の呼吸器に及ぼす影響に関する形態学的研究(第614回千葉医学会例会・第14回肺癌研究施設例会)

<p>The first two columns are the protein IDs of the JGI <i>P</i>. <i>ostreatus</i> genome database.</p

Distribution of GO-slim annotations of putative gene products predicted from switchgrass BAC-end sequences.

<p>A, Cellular locations −12 groups of gene ontology; B, Biological processes −11 groups of gene ontology; C, Molecular functions −15 groups of gene ontology terms.</p

Characteristics of the switchgrass BAC libraries.

<p>Characteristics of the switchgrass BAC libraries.</p

Analysis of Simple Sequence Repeats (SSRs) from BAC-end sequences.

<p><b>a</b>) Distribution of total number of repeat loci. The x-axis represents the length of SSRs and the y-axis indicates total number of motifs observed. <b>b</b>) Distribution of SSR trimers. X-axis represents the various trinucleotide SSRs and the y-axis represents number of SSRs.</p

Distribution of BAC end sequences that show significant homology to other BES.

<p>The x-axis represents the number of matches and y-axis contains total number of BAC-end sequences.</p

Micro-collinearity between switchgrass BAC clones and orthologous regions from <i>Brachypodium</i> (<i>Bd</i>), rice (<i>Os</i>), sorghum (<i>Sb</i>) and maize (<i>Zm</i>).

<p>Colored boxes along the physical location in the genome of each species represent genes and arrows in the colored boxes indicate the transcriptional orientation of each gene. Orthologous genes are given the same color and are connected by dotted lines. Grey bars represent genes from respective genomes lacking syntenic match in switchgrass. Dashed lines represent breaks in contiguity to allow larger genomic regions of the chromosomes to fit in the scale of the figures and the genes from these regions lacking syntenic match have not been plotted. The scale is shown at the bottom of each section. NCBI accession numbers for switchgrass BAC clones are given at top left of each section. Detailed information on accession numbers and gene names is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033892#pone.0033892.s008" target="_blank">File S7</a>.</p

Estimation of organellar DNA contamination and representation of low-copy genes.

<p>Switchgrass BAC libraries (Pv_ABa and Pv_ABb) were screened by high-density filter hybridizations to estimate chloroplast- or mitochondrial-specific DNA and representation of single/low copy genes. Representative filter hybridization data used to estimate chloroplast (A, D) and mitochondrial (B, E) contaminants, and the library coverage based on the presence of a single copy gene, <i>brittle culm 10</i> (C, F). Black arrows in C and F identify the signal from <i>BC10</i> probes.</p

Determination and distribution of switchgrass BAC clone insert sizes.

<p>DNA was digested from >180 randomly selected BAC clones from Pv_ABa and Pv_ABb libraries and analyzed by Pulsed Field Gel Electrophoresis. A, C), Representative gel pictures of <i>Not</i>I digested BAC DNA from Pv_ABa and Pv_ABb libraries, respectively. B, D) Estimated BAC insert sizes with their relative frequencies.</p

Molecular validation of polymorphic insertions in PC15 and PC9 strains.

<p>Primers I to VIII were designed to flank heterozygous TE insertions (present only in one of the two genomes for a given locus) and were used to amplify the target loci in both strains (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006108#pgen.1006108.s010" target="_blank">S1 Text</a>). Panel (A) shows TE insertions in PC9 strain, and panel (B) shows TE insertions in PC15.</p

Phylogenetic reconstruction of TIR_1-like Tc1-mariner transposases.

<p>Basidiomycete, ascomycete, animal, and bacterial Tc1-mariner transposases are shown in dark blue, light blue, orange and red, respectively. SH indices are included indicating branch support.</p