2,494 research outputs found
Mechanisms of RNA loading into exosomes
AbstractUpon fusion of multivesicular bodies (MVBs) with the plasma membrane, intraluminal vesicles (ILVs) are released into the extracellular space as exosomes. Since the lipid composition of the exosomal membrane resembles that of raft microdomains, the inward budding process involves the raft-like region of the MVB limiting membrane. Although published research suggests that cellular RNAs may be selectively sorted into exosomes, the molecular mechanisms remain elusive. In this review, we suggest that there is a continuous interaction of cellular RNAs with the outer (cytoplasmic) surface of MVBs and that the selection for incorporation of these RNAs into ILVs is based on their affinity to the raft-like region in the outer layer of the MVB membrane
The Ethics and Bundaries of Industry Environmental Campaigns
As scientific consensus points to an environmental crisis, many industry campaigns downplay the industry’s environmental impacts while publicizing adverse effects to the economy that may result from environmental regulations. This project identifies boundaries of responsible industry environmental in terms of the ethical criteria of visibility, veracity, and sensitivity
Asymptotics of large eigenvalues for a class of band matrices
We investigate the asymptotic behaviour of large eigenvalues for a class of
finite difference self-adjoint operators with compact resolvent in
Effect of carbon addition on functional and mechnical properties of the Ni3Al phase
Casting technology was applied and research was carried out on two alloys based on the Ni3Al phase with variable carbon content of 0,2 and 1,25 wt. % C, respectively. Resistance to abrasive wear, friction coefficient and Vickers microhardness were determined. Metallographic studies were conducted to examine the macrostructure and microstructure of the investigated alloys. An increase in resistance to abrasive wear and microhardness of alloy containing 1,25 wt. % C was observed. The coefficient of friction was determined, which for the alloy with increased carbon content was much lower than for the alloy containing 0,2 wt. % C. Structural changes were reported to have some effect on functional and mechanical properties of the examined alloys
Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
AbstractHuman immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection
Decay of proton-rich nuclei between 39Ti and 49Ni
Decay studies of very neutron-deficient nuclei ranging from 39Ti to 49Ni have
been performed during a projectile fragmentation experiment at the GANIL/LISE3
separator. For all nuclei studied in this work, 39,40Ti, 42,43Cr, 46Mn,
45,46,47Fe and 49Ni, half-lives and decay spectra have been measured. In a few
cases, gamma coincidence measurements helped to successfully identify the
initial and final states of transitions. In these cases, partial decay scheme
are proposed. For the most exotic isotopes, 39Ti, 42Cr, 45Fe and 49Ni, which
are candidates for two-proton radioactivity from the ground state, no clear
evidence of this process is seen in our spectra and we conclude rather on a
delayed particle decay.Comment: 12 pages, 15 figures, submitted for publication in Eur. Phys. J.
First observation of 55,56Zn
In an experiment at the SISSI/LISE3 facility of GANIL, the most proton-rich
zinc isotopes 55,56Zn have been observed for the first time. The experiment was
performed using a high-intensity 58Ni beam at 74.5 MeV/nucleon impinging on a
nickel target. The identification of 55,56Zn opens the way to 54Zn, a good
candidate for two-proton radioactivity according to theoretical predictions.Comment: 2 pages, 1 figure, accepted for publication in Eur. Phys. J.
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