Schematic representation of HIV Nef ELISA optimization.

<p>Key assay optimizations are highlighted by the red text. Three linear standard curves of serially diluted Nef in negative serum (1.25 ng/ml to 50 ng/ml), designated as S1, S2 and S3, are shown as assay readouts. rNef, recombinant Nef; RT, room temperature; HRP-Ab, horseradish peroxidase-conjugated antibody; TBM, 3,3',5,5'-tetramethylbenzidine; A, absorbance. For details, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191613#sec007" target="_blank">Materials and methods</a>.</p

Clinical characteristics of study participants.

<p>Clinical characteristics of study participants.</p

Additional file 2: Table S2. of N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration

Primer sequences used for qRT-PCR, RACE, siRNAs, and vector construction in this study. (XLSX 11 kb