Collection, Recycling and Recovery of Concrete Waste in the Company HERKUL a. s.

Bakalářská práce charakterizuje stavební a demoliční odpad a problematiku spojenou s jeho recyklací. Popisuje proces získávání a shromažďování betonového odpadu v recyklačním zařízení společnosti HERKUL a. s. a dále proces recyklace betonového odpadu až po získání konečného výrobku neboli recyklátu a jeho využití ve stavebnictví. Hlavní část je zaměřena na vyčíslení ekonomického přínosu hodnocené recyklační plochy.The bachelor thesis characterizes construction and demolition waste and problems connected to recycling it. It describes the process of gaining and collecting concrete waste at the recycling facility of company HERKUL a. s. and process of recycling concrete waste all the way to the final product – the recyclete and it use in construction. The main part is focused on quantification of the economic benefit of the recycling area.546 - Katedra environmentálního inženýrstvívýborn

Horse flies (Diptera: Tabanidae) collected in Central African Republic, Gabon and Liberia with comments on their updated distribution

A zoogeographical review summarized the current distribution of 28 morphologically determined horse fly species recently collected in three Western Africa countries. In Malaise trap collections, the family Tabanidae was represented by three subfamilies, Pangoniinae, Chrysopsinae and Tabaninae (tribes Philolichini, Chrysopsini, Tabanini and Haematopotini), and six genera: Philoliche Wiedemann, 1828 (1 species), Chrysops Meigen, 1803 (3 specis), Ancala Enderlein, 1922 (2 species), Euancala Enderlein, 1922 (1 species), Tabanus Linnaeus, 1758 (13 species) and Haematopota Meigen, 1803 (8 species). Information contained in the principal publication The Catalogue of the Diptera of the Afrotropical Region 1980 is complemented by newly published studies on horse flies in Sub-Saharan Africa

Avian Louse Flies and Their Trypanosomes: New Vectors, New Lineages and Host–Parasite Associations

Louse flies (Hippoboscidae) are permanent ectoparasites of birds and mammals. They have a cosmopolitan distribution with more than 200 described species. The aim of this study was to reveal host–vector–parasite associations between louse flies, birds, and trypanosomes. A total of 567 louse fly specimens belonging to 7 species were collected from birds at several localities in Czechia, including the rare species Ornithophila metallica and Ornithoica turdi. There was a significant difference in the occurrence of Ornithomya avicularia and Ornithomya fringillina on bird hosts according to their migratory status, O. fringillina being found more frequently on long-distance migrants. Trypanosomes were found in four species, namely, Ornithomya avicularia, O. fringillina, O. biloba, and Ornithoica turdi; the later three species are identified in this paper as natural trypanosome vectors for the first time. The prevalence of trypanosomes ranged between 5 and 19%, the highest being in O. biloba and the lowest being in O. fringillina. Phylogenetic analysis of the SSU rRNA gene revealed that a vast majority of trypanosomes from hippoboscids belong to the avian T. corvi/culicavium group B. Four new lineages were revealed in group B, with louse flies being probable vectors for some of these trypanosome lineages. We also confirmed the transcontinental distribution of several trypanosome lineages. Our results show that hippoboscids of several genera are probable vectors of avian trypanosomes

Trypanosomes of the Trypanosoma theileri Group: Phylogeny and New Potential Vectors

Trypanosomes belonging to Trypanosoma theileri group are mammalian blood parasites with keds and horse fly vectors. Our aim is to study to vector specificity of T. theileri trypanosomes. During our bloodsucking Diptera survey, we found a surprisingly high prevalence of T. theileri trypanosomes in mosquitoes (154/4051). Using PCR and gut dissections, we detected trypanosomes of T. theileri group mainly in Aedes mosquitoes, with the highest prevalence in Ae. excrucians (22%), Ae. punctor (21%), and Ae. cantans/annulipes (10%). Moreover, T. theileri group were found in keds and blackflies, which were reported as potential vectors for the first time. The vectorial capacity was confirmed by experimental infections of Ae. aegypti using our isolates from mosquitoes; sand fly Phlebotomus perniciosus supported the development of trypanosomes as well. Infection rates were high in both vectors (47–91% in mosquitoes, 65% in sandflies). Furthermore, metacyclic stages of T. theileri trypanosomes were observed in the gut of infected vectors; these putative infectious forms were found in the urine of Ae. aegypti after a second bloodmeal. On the contrary, Culex pipiens quinquefasciatus was refractory to experimental infections. According to a phylogenetic analysis of the 18S rRNA gene, our trypanosomes belong into three lineages, TthI, ThII, and a lineage referred to as here a putative lineage TthIII. The TthI lineage is transmitted by Brachycera, while TthII and ThIII include trypanosomes from Nematocera. In conclusion, we show that T. theileri trypanosomes have a wide range of potential dipteran vectors, and mosquitoes and, possibly, sandflies serve as important vectors

Blood parasites (Trypanosoma, Leucocytozoon, Haemoproteus) in the Eurasian sparrowhawk (Accipiter nisus): diversity, incidence and persistence of infection at the individual level

Abstract Background A high prevalence of parasites may result from life-long persistence of infection or from high reinfection rates. We have studied blood parasites in a breeding population of the accipitrid raptor, Eurasian sparrowhawk (Accipiter nisus), to determine parasite diversity and turnover. Methods During this 7-year study, 210 adult Eurasian sparrowhawks breeding in the city of Prague were checked for parasites using several diagnostic methods. Results In both female and male raptors, parasites of the genus Leucocytozoon were the most prevalent (92% and 85%, respectively) followed in decreasing order of prevalence by those of genus Trypanosoma (74% and 68%, respectively) and genus Haemoproteus (46% and 16%, respectively). The prevalence of all parasites increased with age in both sexes, with the females at each respective age having the higher prevalence. There was a positive association between Haemoproteus and Leucocytozoon infections. Persistence at the individual level was higher than incidence for Trypanosoma and Haemoproteus. In the case of Leucocytozoon and Trypanosoma, most individuals probably become infected in their first year of life or even before dispersal from the nest. The detected parasites belonged to Trypanosoma avium sensu stricto, Leucocytozoon sp. (haplotypes ACNI1 and ACNI3) and Leucocytozoon mathisi (haplotype ACNI4) and two new lineages of the Haemoproteus elani complex (ACCNIS6 and ACCNIS7). Detailed analysis of parasite lineages in individuals that were repeatedly sampled revealed lineage turnover that would otherwise remain hidden. Phylogenetic analysis revealed that the detected Haemoproteus belongs to a phylogenetically distant group whose taxonomic position requires further analysis. Conclusions All three genera of blood parasites persist in infected individuals, thus enabling sustainability of vector transmission cycles. Prevalence increases with age; however, there is a high turnover of Leucocytozoon lineages. No clear evidence of parasite-induced mortality was found, and most of the individuals were infected early in life, particularly in the case of Leucocytozoon. Graphical abstrac