Phenotypic flexibility and the evolution of organismal design

Evolutionary biologists often use phenotypic differences between species and between individuals to gain an understanding of organismal design. The focus of much recent attention has been on developmental plasticity – the environmentally induced variability during development within a single genotype. The phenotypic variation expressed by single reproductively mature organisms throughout their life, traditionally the subject of many physiological studies, has remained underexploited in evolutionary biology. Phenotypic flexibility, the reversible within-individual variation, is a function of environmental conditions varying predictably (e.g. with season), or of more stochastic fluctuations in the environment. Here, we provide a common framework to bring the different categories of phenotypic plasticity together, and emphasize perspectives on adaptation that reversible types of plasticity might provide. We argue that better recognition and use of the various levels of phenotypic variation will increase the scope for phenotypic experimentation, comparison and integration.

Development and evaluation of land use regression models for black carbon based on bicycle and pedestrian measurements in the urban environment

Land use regression (LUR) modelling is increasingly used in epidemiological studies to predict air pollution exposure. The use of stationary measurements at a limited number of locations to build a LUR model, however, can lead to an overestimation of its predictive abilities. We use opportunistic mobile monitoring to gather data at a high spatial resolution to build LUR models to predict annual average concentrations of black carbon (BC). The models explain a significant part of the variance in BC concentrations. However, the overall predictive performance remains low, due to input uncertainty and lack of predictive variables that can properly capture the complex characteristics of local concentrations. We stress the importance of using an appropriate cross-validation scheme to estimate the predictive performance of the model. By using independent data for the validation and excluding those data also during variable selection in the model building procedure, overly optimistic performance estimates are avoided. (C) 2017 Elsevier Ltd. All rights reserved

Spring migration of Ruffs Philomachus pugnax in Fryslân: estimates of staging duration using resighting data

Seasonal bird migration involves long flights, but most time is actually spent at intermediate staging areas. The duration of stay at these sites can be evaluated with mark–recapture methods that employ day-to-day local encounters of individually marked birds. Estimates of staging duration are based on two probabilities: the immigration probability, the complement of a bird’s seniority to an area, and the emigration probability, the complement of the staying probability. Estimating total staging duration from seniority and staying probabilities requires validation for resighting data and here we compare three data categories of Ruffs Philomachus pugnax passing through The Netherlands during northward migration: (1) newly colour-ringed, (2) previously colour-ringed and (3) radio-tagged Ruffs (recorded by automated receiving stations). Between 2004 and 2008, 4363 resighting histories and 95 telemetry recording histories were collected. As sample sizes for females were low, only data for males were analysed. Possible catching effects affecting estimates of staging duration were explored. Staying probability was estimated for all data. Seniority however, could not be estimated for newly marked Ruffs; the assumption of equal ‘capture’ probability for reverse-time models applied to estimate seniority is violated for seasonal resighting histories starting with a catching event. Therefore, estimates of total staging duration were based on resightings of previously colour-marked birds only. For radio-tagged birds a minimal staging duration (time between tagging and last recording) was calculated. Modelling indicated that newly colour-ringed birds had a higher staying probability than previously colour-ringed birds, but the difference translated to a prolonged staging duration in newly ringed birds of only 0.4–0.5 d, suggesting a very small catching effect. The minimal staging duration of radio-tagged birds validated estimates of staging duration for colour-ringed birds in 2007 but not in 2005. In 2005 a low resighting probability resulted in underestimates of staging duration. We conclude that (1) estimates of staying probability can be affected by catching although effects on staging duration might be small, and that (2) low resighting probabilities can lead to underestimates in staging duration. In our study previously ringed Ruffs resighted in 2006–08 yielded reliable estimates of staging duration as data had sufficiently high resighting probabilities. Average staging durations varied between 19 d in 2008 and 23 d in 2006.

Development of a land use regression model for black carbon using mobile monitoring data and its application to pollution-avoiding routing

Black carbon is often used as an indicator for combustion-related air pollution. In urban environments, on-road black carbon concentrations have a large spatial variability, suggesting that the personal exposure of a cyclist to black carbon can heavily depend on the route that is chosen to reach a destination. In this paper, we describe the development of a cyclist routing procedure that minimizes personal exposure to black carbon. Firstly, a land use regression model for predicting black carbon concentrations in an urban environment is developed using mobile monitoring data, collected by cyclists. The optimal model is selected and validated using a spatially stratified cross-validation scheme. The resulting model is integrated in a dedicated routing procedure that minimizes personal exposure to black carbon during cycling. The best model obtains a coefficient of multiple correlation of R = 0.520. Simulations with the black carbon exposure minimizing routing procedure indicate that the inhaled amount of black carbon is reduced by 1.58% on average as compared to the shortest-path route, with extreme cases where a reduction of up to 13.35% is obtained. Moreover, we observed that the average exposure to black carbon and the exposure to local peak concentrations on a route are competing objectives, and propose a parametrized cost function for the routing problem that allows for a gradual transition from routes that minimize average exposure to routes that minimize peak exposure

The effect of different reinforcements on the fracture toughness of materials for interim restorations

STATEMENT OF PROBLEM. Fracture of an interim fixed partial denture (FPD) may jeopardize the success of the interim prosthodontic treatment phase and cause patient discomfort. PURPOSE. The purpose of this study was to compare the fracture toughness of a polymethyl methacrylate (PMMA) resin and a bis-acryl composite (BAC) resin reinforced with stainless steel wire, glass, and polyethylene fiber. MATERIAL AND METHODS. Four groups (n=13) of each of the 2 materials were prepared for the single-edge 3-point-bending test. Three groups had the different reinforcements, and the group without reinforcement served as control. Using a universal testing machine, peak load to fracture was recorded and fracture toughness (KIC) was calculated in MNm-1.5. Median KIC values were compared by means of nonparametric ANOVA (Kruskal-Wallis test, α=.05). RESULTs. For the controls, the fracture toughness for PMMA resin (KIC=27.9) was significantly lower (P.10, for BAC resin and KIC=25.8, P>.10, for PMMA resin). There was no significant difference between the fracture toughness of the wire and glass fiber reinforcements for both interim materials (P>.10 in both instances). CONCLUSIONS. Of the 3 reinforcement methods evaluated, wire and glass fiber reinforced the PMMA and BAC resin materials best.Web of Scienc

Radio-telemetry observations of the first 650 km of the migration of Bar-tailed Godwits Limosa lapponica from the Wadden Sea to the Russian Arctic

In 1999 and 2000, 45 Bar-tailed Godwits Limosa lapponica were supplied with radio-transmitters during spring staging on the island Texel in the western Wadden Sea. With the use of Automatic Radio Tracking Stations (ARTS) on Texel and in south Sweden, and hand-held receivers on Texel, it was possible to follow the later part of the stopover period on Texel for 34 birds (76%) and the passage over south Sweden for 26 birds (58%). Thus, the method of automatic tracking of overflying migrating shorebirds works successfully where the migration corridor is narrow and predictable, as in the case with late spring shorebird migration from the Wadden Sea towards arctic Russia. The timing of departure from Texel and passage over south Sweden of radio-marked birds, with median dates of 30 May and 2 June respectively, were in agreement with published data on the spring migration of Siberian-breeding Bar-tailed Godwits L. l. taymyrensis. The individual variation in migration dates was larger than expected, with birds passing south Sweden between 25 May and 10 June, indicating that the time-window for departure might be broader than previously thought. There was no clear difference between males and females in timing of migration. The time difference between departure from Texel and passage over south Sweden (average 3.3 days) indicates that most Bar-tailed Godwits do not embark on the long flight towards Siberia directly from the western Wadden Sea, but are more likely to stop in the more easterly portion of the Wadden Sea before the final take-off. This pattern is similar to what has been found in other shorebirds and geese (e.g. Red Knots Calidris canutus and Dark-bellied Brent Geese Branta bernicla) migrating along the same route.