5 research outputs found
Mitochondrial biogenesis and COX activity are impaired in frail old.
<p>(A) PGC-1Ξ±, CS, COX subunits- I, II, and IV, and phospho-GSK3Ξ² (Ser<sup>9</sup>) protein content, (B) citrate synthase and (C) mitochondrial complex IV activity (nmol.min<sup>β1</sup>.mg of protein<sup>β1</sup>) in the <i>vastus lateralis</i> of young, AO, and SO subjects (Nβ=β10/group; β β=β β). (D) Mitochondrial complex IV activity (nmol.min<sup>β1</sup>.mg of protein<sup>β1</sup>) positively correlates (rβ=β0.77) with maximal isometric torque (N.m). Asterisk denotes significant changes <i>vs.</i> young, dagger denotes significant changes <i>vs.</i> AO, and double dagger denotes significant changes <i>vs.</i> both young and AO (Pβ€0.05).</p
A sedentary lifestyle exacerbates functional decline and systemic inflammation in the elderly.
<p>(A) Maximal isometric torque (N.m) in young, AO, and SO subjects. Each point represents an individual who underwent strength testing as described in the methods. (B) Walk test and timed stair-climb test in AO and SO subjects as described in the methods. (C) CRP levels (ng.mg of protein<sup>β1</sup>) in the <i>vastus lateralis</i> of young, AO, and SO subjects (Nβ=β10/group; β β=β β). Asterisk denotes significant changes <i>vs.</i> young, and dagger denotes significant changes <i>vs.</i> AO (Pβ€0.05).</p
Reduced Mn-SOD activity is due to tyrosine nitration in frail old.
<p>(A) Mn-SOD was immunoprecipitated (IP) followed by immunoblotting (IB) for Mn-SOD nitrotyrosine content and (B) nNOS protein content in the <i>vastus lateralis</i> of young, AO, and SO subjects (Nβ=β10/group; β β=β β). Asterisk denotes significant changes <i>vs.</i> young, and dagger denotes significant changes <i>vs.</i> AO (Pβ€0.05).</p
Study subjects' characteristics.
<p>Values are means Β± SD. <sup>β‘</sup>β β=β β; <sup>*</sup> significantly different from young and AO (P<0.05); <sup>β </sup>significantly different from young (P<0.05).</p
Mitochondrial Mn-SOD activity is reduced in frail old.
<p>(A) Mn-SOD protein content and (B) total- and Mn- SOD activity (U.mg of protein<sup>β1</sup>) in the <i>vastus lateralis</i> of young, AO, and SO subjects (Nβ=β10/group; β β=β β). Asterisk denotes significant changes <i>vs.</i> young, and dagger denotes significant changes <i>vs.</i> AO (Pβ€0.05).</p