Displaying the status of industrial devices and their supporting data in augmented reality

U průmyslových zařízení je diagnostika jednou z nejdůležitějších částí, díky její správné funkčnosti je možné zabránit ztrátám ve výrobě. Nový pracovníci si často musí projít několik školení, aby pochopili, jak funguje použitý typ diagnostického systému v továrně a uměli ho správně využít. Nejen s urychlením procesu školení pracovníka může pomoci aplikace popsaná v této práci. Práce má za cíl vytvořit funkční prototyp aplikace pro přenos dat z průmyslových zařízení do zobrazovacího zařízení HoloLens 2 a přijatá data zobrazit na konkrétních místech v prostoru, podle toho, kde se odesílající průmyslové zařízení nachází. Může jít jak o pevně umístěná, tak o pohybující se průmyslová zařízení, jejichž detekci zajišťuje software Vuforia. U zobrazování je možné vybrat typ zobrazení datových zpráv podle nastavených vzorů. Samotnou komunikaci zajišťuje protokol MQTT a zasílané zprávy jsou rozděleny na datové a kontrolní. Na závěr bylo provedeno několik zátěžových testů, které ověřili kvalitu přijímaných a odesílaných zpráv. Pro každý druh zařízení jsou jednotlivé programy napsány v jazyku C# a vývoj pro HoloLens 2 byl možný díky Unity Editoru a knihovně MRTK.For industrial devices, the diagnostic system is one of the most important parts of the industrial facility. If it works well, it can prevent loss in production. The new worker must often complete several training courses to understand how the diagnostic system works and how to use it. The system described in this thesis can help with the worker’s training. This thesis aims to create a functional prototype application for data transmission from industrial devices to the visualization device HoloLens 2. It shows the data on the specific places in the facility according to the location of the transmitted industrial devices. Industrial machines can be both fixed or mobile. Their detection ensures Vuforia software. For the visualization, the user can choose the type of data visualization according to offered patterns. The communication provides MQTT protocol, and the messages are divided into two groups control and data. In the evaluation part, several verifying tests that validated the quality of transmission were done. The individual programs are written for every kind of device in C#, and the HoloLens 2 development is possible thanks to Unity editor and the MRTK library

Intelligent Cell Spray System

Tato práce popisuje proces vývoje inteligentního rozprašovače kožních buněk používaného pro léčbu různých druhů popálenin. Hlavním úkolem zařízení je rovnoměrné rozdělení léčivé látky po popálenině. Práce diskutuje několik možností řešení takového problému, mezi které patří odhad rychlosti pohybu zařízení při nástřiku nebo určení absolutní polohy vůči tělu pacienta. Právě poslední možnost řešení se jeví jako nejlepší z ohledem na kvalitu postřiku, navíc nabízí další benefity které pomůžou vývoji léčivé látky. Je ovšem potřeba vyřešit i další problémy spojené s požadavky operace, například dodržení stejné vzdálenosti mezi tělem pacienta a aplikátorem, nebo možnost použít zařízení jednoduše bez předchozího školení. Během projektu byli postaveny dva prototypy, při jejich vývoji byli jednotlivé problémy diskutovány s chirurgy, kteří poskytli svůj pohled odborníka na danou problematiku, zároveň přesně definovali problémy, které by toto zařízení mělo vyřešit.This thesis describes the process of developing an intelligent skin cell sprayer used to treat various types of burns. The main task of the device is to distribute the therapeutic substance over the burn evenly. Several possible solutions are described for such a problem which include estimating the speed of movement of the device during application and determining the absolute position relative to the patient’s body. The second solution seems to be ideal in terms of spray quality and offers additional benefits that will help develop the therapeutic substance. However, other issues related to the operation requirements need to be addressed, such as maintaining a consistent distance between the patient’s body and the applicator. Or perhaps the ability to use the device easily and effectively without prior training. During the project, two prototypes were built. Individual problems were discussed with surgeons, who provided expert perspectives on the issue and precisely defined the problems that this device should solve

Early Expression of FcγRI (CD64) on Monocytes of Cardiac Surgical Patients and Higher Density of Monocyte Anti-Inflammatory Scavenger CD163 Receptor in “On-Pump” Patients

Objective. Activation of innate immunity cells is inseparably linked to cardiac surgical operation. The aim of this study was to assess the kinetics in the expression of receptor for Fc part of IgG, FcγRI (CD64), and scavenger receptor CD163 on peripheral blood cells of cardiac surgical patients and to examine the effect of cardiac bypass as a separable influence on the systemic acute inflammatory response. Methods. Forty patients, twenty in each group, were randomly assigned to CABG surgery performed either with “on-pump” or without “off-pump” cardiopulmonary bypass. Standardized quantitative flow cytometry method was used to determine the expression of surface markers. Results. The density of CD64 molecule on monocytes reached maximum on the 1st postoperative day (P<.001) whereas the peak for CD64 molecule expression on granulocytes was postponed to the 3rd postoperative day (P<.001). The expression of CD163 scavenger molecule on monocytes reached maximum on the 1st postoperative day (P<.001). The density of CD163 molecule on monocytes on the 1st postoperative day is significantly higher in “on-pump” patients in comparison with “off-pump” patients (P<.001). Conclusion. In cardiac surgical patients the expression of activation marker FcγR1 (CD64) on monocytes is increased earlier in comparison with granulocytes in both “on-pump” and “off-pump” patients. The expression of scavenger molecule CD163 on monocytes is significantly higher in “on-pump” patients

Lipopolysaccharide Binding Protein and sCD14 are Not Produced as Acute Phase Proteins in Cardiac Surgery

Objectives. The changes in the serum levels of lipopolysaccharide binding protein (LBP) and sCD14 during cardiac surgery were followed in this study. Design. Thirty-four patients, 17 in each group, were randomly assigned to coronary artery bypass grafting surgery performed either with (“on-pump”) or without (“off-pump”) cardiopulmonary bypass. LBP and sCD14 were evaluated by ELISA. Results. The serum levels of LBP were gradually increased from the 1st postoperative day and reached their maximum on the 3rd postoperative day in both “on-pump” and “off-pump” patients (30.33±9.96 μg/mL; 37.99±16.58 μg/mL), respectively. There were no significant differences between “on-pump” and “off-pump” patients regarding LBP. The significantly increased levels of sCD14 from the 1st up to the 7th postoperative day in both “on-pump” and “off-pump” patients were found with no significant differences between these groups. No correlations between LBP and sCD14 and IL-6, CRP and long pentraxin PTX3 levels were found. Conclusions. The levels of LBP and sCD14 are elevated in cardiac surgical patients being similar in both groups. These molecules are not produced as acute phase proteins in these patients

TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis

The aim of this study was to examine the role of TLR2 molecule in pleural space during thoracoscopic talc pleurodesis period in patients with malignant pleural effusion. We analyzed TLR2 molecule in soluble form as well as on membrane of granulocytes in pleural fluid. Pleural fluid examination was done at three intervals during pleurodesis procedure: 1st—before the thoracoscopic procedure, 2nd—2 hours after the terminating thoracoscopic procedure with talc insufflation, 3rd—24 hours after the thoracoscopic procedure. We reported significant increase of soluble TLR2 molecule in pleural fluid effusion during talc pleurodesis from preoperative value. This increase was approximately 8-fold in the interval of 24 hours. The changes on granulocyte population were quite different. The mean fluorescent intensity of membrane TLR2 molecule examined by flow cytometry on granulocyte population significantly decreased after talc exposure with comparison to prethoracoscopic density. To estimate the prognostic value of TLR2 expression in pleural fluid patients were retrospectively classified into either prognostically favourable or unfavourable groups. Our results proved that patients with favourable prognosis had more than 3-fold higher soluble TLR2 level in pleural fluid early, 2 hours after talc pleurodesis intervention

Oxidative Damage to Nucleic Acids and Benzo(a)pyrene-7,8-diol-9,10-epoxide-DNA Adducts and Chromosomal Aberration in Children with Psoriasis Repeatedly Exposed to Crude Coal Tar Ointment and UV Radiation

The paper presents a prospective cohort study. Observed group was formed of children with plaque psoriasis (n = 19) treated by Goeckerman therapy (GT). The study describes adverse (side) effects associated with application of GT (combined exposure of 3% crude coal tar ointment and UV radiation). After GT we found significantly increased markers of oxidative stress (8-hydroxy-2 -deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), significantly increased levels of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) DNA adducts (BPDE-DNA), and significantly increased levels of total number of chromosomal aberrations in peripheral lymphocytes. We found significant relationship between (1) time of UV exposure and total number of aberrated cells and (2) daily topical application of 3% crude coal tar ointment (% of body surface) and level of BPDE-DNA adducts. The findings indicated increased hazard of oxidative stress and genotoxic effects related to the treatment. However, it must be noted that the oxidized guanine species and BPDE-DNA adducts also reflect individual variations in metabolic enzyme activity (different extent of bioactivation of benzo[a]pyrene to BPDE) and overall efficiency of DNA/RNA repair system. The study confirmed good effectiveness of the GT (significantly decreased PASI score)

Oxidative Damage to Nucleic Acids and Benzo(a)pyrene-7,8-diol-9,10-epoxide-DNA Adducts and Chromosomal Aberration in Children with Psoriasis Repeatedly Exposed to Crude Coal Tar Ointment and UV Radiation

The paper presents a prospective cohort study. Observed group was formed of children with plaque psoriasis (n=19) treated by Goeckerman therapy (GT). The study describes adverse (side) effects associated with application of GT (combined exposure of 3% crude coal tar ointment and UV radiation). After GT we found significantly increased markers of oxidative stress (8-hydroxy-2′-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), significantly increased levels of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) DNA adducts (BPDE-DNA), and significantly increased levels of total number of chromosomal aberrations in peripheral lymphocytes. We found significant relationship between (1) time of UV exposure and total number of aberrated cells and (2) daily topical application of 3% crude coal tar ointment (% of body surface) and level of BPDE-DNA adducts. The findings indicated increased hazard of oxidative stress and genotoxic effects related to the treatment. However, it must be noted that the oxidized guanine species and BPDE-DNA adducts also reflect individual variations in metabolic enzyme activity (different extent of bioactivation of benzo[a]pyrene to BPDE) and overall efficiency of DNA/RNA repair system. The study confirmed good effectiveness of the GT (significantly decreased PASI score).</jats:p

Clinical Study Selected Inflammatory and Metabolic Markers in Psoriatic Patients Treated with Goeckerman Therapy

Psoriasis is associated with metabolic activity of adipose tissue which produces pro-and anti-inflammatory adipokines. Goeckerman therapy (GT) represents an effective treatment of psoriasis. This study evaluated variation of selected inflammatory and metabolic markers during GT and the relationships between the markers, severity of the disease (PASI score), body mass, and the basic characteristics of the therapy. The study was conducted on a group of patients ( = 32) and on a control group ( = 24). Before GT, we found significantly elevated levels of proinflammatory CRP ( &lt; 0.001) and leptin ( &lt; 0.05) in psoriatic patients (compared to the controls). The therapy significantly decreased the levels of CRP and adiponectin. We found positive correlations between CRP and total duration of GT ( &lt; 0.05) and CRP and the time of UV exposure ( &lt; 0.01) and negative correlations between adiponectin and the total duration of GT ( &lt; 0.05) and adiponectin and the application of CCT ointment ( &lt; 0.001). From our results, we can conclude that GT causes partial reduction of both proinflammatory and anti-inflammatory markers. However, the levels of proinflammatory CRP and leptin remained significantly higher in the patients than in the control group

Development of submicromolar 17β-HSD10 inhibitors and their in vitro and in vivo evaluation

Funding: This study was supported by the Ministry of Education, Youth, and Sports of the Czech Republic (project ESF no. CZ.02.1.01/0.0/0.0/18_069/0010054), by the University of Hradec Kralove (Faculty of Science, no. SV2103‐2022), by Charles University, Prague, Czech Republic (project Cooperatio, research area Neurosciences), by the project MH CZ-DRO VFN64165, and by MH CZ - DRO (UHHK, 00179906), by the Ministry of Defence of the Czech Republic (Faculty of Military Health Sciences Hradec Kralove) under the grant entitled the “Long-term organization development plan - Medical Aspects of Weapons of Mass Destruction”, and by the RS MacDonald Charitable Trust and Rosetrees Trust.17β-hydroxysteroid dehydrogenase type 10 (17β-HSD10) is a multifunctional mitochondrial enzyme and putative drug target for the treatment of various pathologies including Alzheimer's disease or some types of hormone-dependent cancer. In this study, a series of new benzothiazolylurea-based inhibitors were developed based on the structure-activity relationship (SAR) study of previously published compounds and predictions of their physico-chemical properties. This led to the identification of several submicromolar inhibitors (IC50 ∼0.3 μM), the most potent compounds within the benzothiazolylurea class known to date. The positive interaction with 17β-HSD10 was further confirmed by differential scanning fluorimetry and the best molecules were found to be cell penetrable. In addition, the best compounds weren't found to have additional effects for mitochondrial off-targets and cytotoxic or neurotoxic effects. The two most potent inhibitors 9 and 11 were selected for in vivo pharmacokinetic study after intravenous and peroral administration. Although the pharmacokinetic results were not fully conclusive, it seemed that compound 9 was bioavailable after peroral administration and could penetrate into the brain (brain-plasma ratio 0.56).Peer reviewe

The Influence of Locality on Phenolic Profile and Antioxidant Capacity of Bud Extracts

Gemmotherapy represents the most recent therapeutic technique that uses the properties of extracts from fresh meristematic plant tissues, mainly buds and sprouts, by macerating them in ethanol and glycerol. The harvesting time and the location can significantly affect the chemical composition of the buds. Therefore, this work aimed to point out the possible variability in the phenolic content and the antioxidant potential of extracts prepared from commonly grown trees in the Czech Republic. Extracts from buds collected during autumn and spring in three different localities were analysed using UHPLC-MS (ultra-high-pressure liquid chromatography) for the phenols profile. Five tests assays were used for the evaluation of the extract antioxidant potential. The sampling time positively affected the content of total phenols, flavonoids, and phenolic acids. The increased levels of total phenols and flavonoids in localities with high and medium pollution may be the result of the higher levels of NO and SO2, the main air pollutants. However, surprisingly, the content of phenolic acid showed the highest values in the area with the lowest pollution. The results of antioxidant tests did not completely correlate with the levels of phenolic metabolites, which may be due to the involvement of other active molecules (e.g., ascorbate, tocopherol, or proline) in the antioxidant machinery