Comparative Breeding and Behavioral Responses to Ethinylestradiol Exposure in Wild and Laboratory Maintained Zebrafish (<i>Danio rerio</i>) Populations

Genetic variation has a significant effect on behavior, fitness, and response to toxicants; however, this is rarely considered in ecotoxicological studies. We compared fitness-related behavioral traits, breeding activity, and the effects of exposure to the environmental estrogen ethinylestradiol (EE₂) on reproduction in a laboratory (Wild Indian Karyotype, WIK) strain and a wild-caught population (Bangladesh, BLD01) of <i>Danio rerio</i> (zebrafish). In WIK fish, males with higher observed heterozygocity were more active reproductively and more successful in securing parentage, but these relationships were not apparent in the BLD01 fish. The frequency of reproductive behaviors increased in WIK zebrafish for exposure to 0.4 ng/L EE₂, which was not apparent in the BLD01 zebrafish. The different strains showed the same threshold for hepatic vitellogenin gene (<i>vtg</i>) induction (2.2 ng EE₂/L), but results suggested an elevated response level in the BLD01. There were no effects on total egg production up to 2.2 ng EE₂/L in either population; however, there was reduced egg fertilization rate at 2.2 ng EE₂/L in the BLD01 fish. These results show consistency in the general responses to EE₂ between these two genetically divergent strains of zebrafish, but also illustrate differences in their breeding biology and response sensitivities. These findings highlight the need for due consideration of the source (and genetics) of populations used in ecological risk assessment for accurate comparisons among studies

Salmo trutta genotypes

Genotypes for 15 populations (641 individuals - full-sibs removed) at 23 microsatellite loci in Genalex forma

Kennedy et al. ESM.docx from <i>Symbiodinium</i> biogeography tracks environmental patterns rather than host genetics in a key Caribbean reef-builder, <i>Orbicella annularis</i>

Methodology and Discussion - Table 1(SADIE outputs, describing the indices for evaluating the spatial distribution of each observed <i>Symbiodinium</i>ITS2 type), Table 2(Cluster indices generated by SADIE analysis for symbionts at each site), Table 3(Summary of DISTLM population-scale outputs), Table 4 (Genotype data for the coral host, <i>Orbicella annularis</i>, by site.

Montastraea annularis microsatellite data

Genotype data for 6 microsatellite loci for 871 samples of Montastraea annularis collected from 26 sites across the wider Caribbean are provided. Montastraea samples were collected in the field between 2004 and 2008. Sample ID, region and site name are supplied for each individual. Column headings are detailed in the ReadMe file