Antibody discovery efficiency using different pretreatments.

<p>Antibody discovery efficiency using different pretreatments.</p

Comparison of <i>in vitro</i> and <i>in vivo</i> expression profiles of different promoters.

<p>(A) Luciferase expression levels in HaCat keratinocyte cells shows that CAG promoter greatly outperforms SFFV, EF1α, and UbC promoters (n = 4). (B) Luciferase expression levels in HEK293 cells similar expression from CAG and EF1α promoters (n = 4). (C) Luciferase expression levels in Balb/c mice shows that CAG promoter greatly outperforms SFFV, EF1α, and UbC promoters (n = 6; two shots in three mice). (D) Expression profile of each construct reveals a rapid decline in expression each day after delivery and ultimately, reaching background levels by day 3. Overall, the CAG promoter yields the highest expression at day 1 and day 2 (****: p<0.01, independent t-test).</p

Effect of different skin pretreatment strategies on anti-huCLDN4 Ab response.

<p>(A) Sandpaper treatment results in a significant increase in MFI against HEK293 expressing huCLDN4 compared to other treatments and mock treated control (n = 5; p < 0.001, independent t-test). Samples were analyzed at a 1:100 dilution. (B) A comparison of FACS- and Mirrorball-based screen of hybridoma supernatants on HEK293 huCLDN4 and parental HEK293 cells demonstrates an 89% (51/57) overlap between the two methods.</p

Luciferase expression profiles after gene gun-based DNA delivery.

<p>Luciferase expression profile in Balb/c (A) and C57BL6 (B) mice for three different gas pressures (150, 300, and 400 psi) at days 1, 2, and 3 post-delivery (n = 5 mice).</p

Effect of different skin pretreatment strategies on luciferase expression.

<p>(A) 1% SDS treatment leads to an increase in luciferase expression one day after delivery compared to other treatments and mock treated control (**, ***: p<0.01, independent t-test) (n = 6; two shots in three mice).</p

Comparison of luciferase expression profiles in TNFα, IFNγ, and IFNα receptor knockout (KO) mice.

<p>(A-C) A rapid decline in luciferase expression for the CAG promoter is observed in all three KO strains as well as the wild-type C57BL6 strain (n = 5).</p